Publications by authors named "Dong-ping Yao"
Rice is highly sensitive to cold stress during reproductive developmental stages, and little is known about the mechanisms of cold responses in rice anther. Using the HiSeq™ 2000 sequencing platform, the anther transcriptome of photo thermo sensitive genic male sterile lines (PTGMS) rice Y58S and P64S (Pei'ai64S) were analyzed at the fertility sensitive stage under cold stress. Approximately 243 million clean reads were obtained from four libraries and aligned against the oryza indica genome and 1497 and 5652 differentially expressed genes (DEGs) were identified in P64S and Y58S, respectively.
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- A new group of bocavirus has been discovered in piglet stool samples, showing a notable prevalence of 12.59%.
- Two nearly complete genomes of these viruses, named porcine bocavirus 1 and 2 (PBoV1 and PBoV2), have been sequenced, revealing high similarity to other members of the bocavirus genus.
- Phylogenetic analysis classified these new viruses alongside known bocaviruses and identified unique proteins, which may enhance the understanding of this viral genus.
[Sequence the complete sequence of bocavirus I with SISPA-PCR].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
February 2010
Objective: To sequence the complete sequence of bocavirus I with sequence independent single primer amplification (SISPA-PCR).
Methods: To exclude the co-effection samples, all clinical samples of diarrhea cases were screened with special primers of rotavirus, astrovirus, adenovirus, calicivirus and bocavirus I. The virus were enriched through ultracentrifugation.