[Study on the metabolism of cartilage matrix by the chondrocytes in osteoarthritic condylar cartilage].

Objective: To study the characteristics of cellular metabolism of mandibular condylar chondrocytes in repairing state of osteoarthrosis and investigate its role in the pathogenesis of the disease.

Methods: Temporomandibular joint osteoarthrosis model of rabbits was created by the partial resection of joint disc and confirmed with histological diagnosis. The chondrocytes were harvested from osteoarthritic condylar cartilage in the repairing state and cultured in vitro under the monolayer culture condition.

[A novel cis-acting enhancer element between CKLF and CKLFSF1 genes].

Aim: To explore the roles of the CKLF gene and CKLFSF1 gene sequence (CCS) in transcriptional regulation.

Methods: The target gene fragment was amplified by PCR and then inserted into pGL3-basic and pGL3-SV40 containing luciferase reporter vector gene to construct pGL3-basic-CCS and pGL3-SV40-CCS. Using liposome-mediated method, four recombinant plasmids were respectively transfected into Hela cells.