Spontaneous pneumorrhachis with pneumomediastinum, scrotal emphysema, and extensive subcutaneous emphysema in a patient with pneumonia: A case report and literature review.

Background: Spontaneous pneumorrhachis with pneumomediastinum, scrotal emphysema, and extensive subcutaneous emphysema caused by acute pneumonia are rarely observed in clinical practice.

Case Presentation: Herein, we report a case of a 12-year-old boy with spontaneous pneumorrhachis with pneumomediastinum, scrotal emphysema, and extensive subcutaneous emphysema caused by a severe cough due to mycoplasma pneumonia. This patient neither received invasive or noninvasive ventilator treatment nor surgical treatment before the onset of the disease.

Magnetic resonance spectroscopy on hepatocellular carcinoma after transcatheter arterial chemoembolization.

Background And Objective: Proton magnetic resonance spectroscopy (MRS) of the liver in vivo is in experimental phase. MRS observation on liver cancer after transcatheter arterial chemoembolization (TACE) has seldom been reported. This study was to investigate the value of MRS in assessing the metabolic changes of hepatocellular carcinoma (HCC) after TACE.

[Dicer efficiently converts large dsRNAs into siRNAs suitable for COX-2 gene].

Objective: To report the use of Dicer to cleave double-stranded RNA (dsRNAs) into small interference RNAs (D-siRNAs) that can target multiple sites within an mRNA, and to acquire an new method to cure inflammation of the airway and tumor.

Methods: Using RiboMAX Large Scale RNA Production Systems-SP6 and T7 kit were used to transcribe A549 cell COX-2 DNA into RNA (dsRNAs). We mixed dsRNAs with Dicer in the reaction buffer.

Transfection of human VEGF165 gene into bone marrow mesenchymal stem cells in rats.

Objective: To create a method for transfecting human vascular endothelial growth factor165 (hVEGF165) gene into bone marrow mesenchymal stem cells (MSCs) in rats.

Methods: MSCs of Wistar rats were isolated by density gradient centrifugation and purified based on their ability of adhesion to plastic. Detections of cell surface antigens, including CD34, CD45, CD44, and SH3, were performed using flow cytometry.

[Anticoagulation in chinese patients with carbomedics mechanical prosthetic heart valves].

Objective: To provide some references for defining the Chinese optimal intensity of anticoagulation after mechanical heart valve prostheses replacement.

Methods: For the 178 patients with carbomedics mechanical prosthetic heart valves, the means of INR were compared between the patients with complications and those without complications at the standard of INR1. 4 - 2.

A susceptibility locus at chromosome 3p21 linked to familial nasopharyngeal carcinoma.

Nasopharyngeal carcinoma (NPC) poses one of the serious health problems in southern Chinese, with an incidence rate ranging from 15 to 50/100,000. Chromosome translocation t(1;3) and frequent loss of heterogeneity on short arms of chromosome 3 and 9 have been reported to be associated with NPC, and a genome-wide scan identified an NPC susceptibility locus on chromosome 4p15.1-q12 recently.

[Expression of PTEN, p16, p21, and p53 proteins in lung cancer using tissue microarray and their clinical significance].

Background & Objective: The genesis of lung cancer was associated with mutation or abnormal expression of PTEN, p16, p21, and p53. Tissue microarray provides a high throughout tool for genes expression. But little is reported about expression of PTEN, p16, p21, and p53 in lung cancers with tissue microarray.

[Effect of ischemic preconditioning on myocardial bcl-2, bax, p53 gene expression during ischemia/reperfusion period in rabbits].

Objective: To investigate the effects of ischemic preconditioning on myocardial bcl-2, bax, p53 gene expression during ischemia/reperfusion period in rabbits.

Methods: Twenty four rabbits were randomly allocated to three groups (n = 8), pseudo-operation group(Group P), ischemia/reperfusion group (Group IR) and ischemic preconditioning group(Group IP). Group IR and Group IP were subjected to three hours of left anterior descending coronary artery occlusion followed for three hours of reperfusion.

[Fibrillin-1 gene mutation in Chinese patients with Marfan syndrome and its gene diagnosis by haplotype analysis].

Objective: To analyze fibrillin-1 (FBN(1)) gene mutation in Chinese patients with Marfan syndrome(MFS) and to make a gene diagnosis by haplotype analysis for MFS.

Methods: Nine MFS families were analysed with single strand conformation polymorphism(SSCP) and DNA sequencing. With the use of four primers designed in the flanking sequences of each short-sequence tandem-repeat region in FBN(1) gene, the haplotype-segregation analysis for MFS(B) was performed.

[Surgical treatment of infective endocarditis: a report of 41 cases].

Objective: To study the characteristics and surgical treatment of infective endocarditis.

Methods: In all patients, surgical treatment was performed including aortic valve replacement in 22, mitral valve replacement in 9, combined aortic and mitral valve replacements in 4, tricuspid valve reconstruction in 3, and pulmonary valve repair in 3. Meanwhile, complicated deformities such as ventricular septal defect (VSD), atrial septal defect (ASD), patient ductus arterisus (PDA), ruptared aneuryem of the aortic sinus and right centricular outflow tract obstruction were corrected.