ATO/ATRA/anthracycline-chemotherapy sequential consolidation achieves long-term efficacy in primary acute promyelocytic leukemia.

The combination of all-trans retinoic acid (ATRA) and arsenic trioxide (As2O3, ATO) has been effective in obtaining high clinical complete remission (CR) rates in acute promyelocytic leukemia (APL), but the long-term efficacy and safety among newly diagnosed APL patients are unclear. In this retrospective study, total 45 newly diagnosed APL patients received ATRA/chemotherapy combination regimen to induce remission. Among them, 43 patients (95.

Acute pleural and pericardial effusion induced by chemotherapy in treating chronic myelocytic leukemia.

Background: Report of a rare and serious complication of chemotherapy with daunorubicin and cytarabine (DA regimen).

Methods: We report a special case of a patient diagnosed chronic myeloid leukemia (CML) with accelerated phase, who simultaneously suffered from acute pleural and pericardial effusion while receiving chemotherapeutic treatment with DA regimen.

Results: Following treatment with DA regimen, the patient had the symptoms of chest distress and shortness of breath, followed by respiratory failure and pericardial tamponade.

[Construction of genetically modified dendritic cell vaccine expressing bcr/abl fusion gene and inducing specific cytotoxic T lymphocytes to kill K562 cells in vitro].

Background And Objective: Specific immunological effect mediated by T lymphocytes plays an important role in treating chronic myelocytic leukemia (CML). Dendritic cells (DCs)-based immunotherapy has become popular in treating tumors. This study was to construct DC vaccines by transducing with replication-defective recombinant adenoviruses expressing bcr/abl fusion gene of CML, observe the lethal effects of specific cytotoxic T lymphocytes (CTLs) triggered by genetically modified DC vaccines expressing bcr/abl fusion gene against K562 cells in vitro.

Temporal thermal response of Type II-IR fiber Bragg gratings.

We use the phase mask method to investigate both experimentally and theoretically the temporal thermal response of Type II-IR fiber Bragg gratings inscribed by a femtosecond laser. A fast testing system is developed to measure the thermal response time by means of periodic CO2 laser irradiation, which creates a rapid temperature change environment. The temporal thermal response is found to be independent of the heat power and the heat direction, although the grating produced destroys the axial symmetry of the fiber.

Asymmetric transverse-load characteristics and polarization dependence of long-period fiber gratings written by a focused CO(2) laser.

Asymmetric transverse-load characteristics and the polarization dependence of long-period fiber gratings (LPFGs) written by high-frequency CO(2) laser pulses are investigated in detail. It is demonstrated that the resonant wavelength is dependent on the direction of the applied force and on the polarization state of the input light; however, the coupling strength is independent of these parameters. When a transverse load is applied along different orientations of the LPFG, the resonant wavelength may be shifted toward the longer wavelength, the shorter wavelength, or hardly shifted, whereas the absolute value of peak transmission attenuation is linearly decreased with an increase of the applied transverse load, with almost no sensitivity to the load direction.

CO2 laser-grooved long period fiber grating temperature sensor system based on intensity modulation.

A long period fiber grating (LPFG) temperature sensor system based on intensity modulation is developed. The LPFG employed is fabricated by the use of a focused CO2 laser beam to carve periodic grooves on the fiber. The temperature measurement resolution of up to 0.

Measuring optical fiber length by use of a short-pulse optical fiber ring laser in a self-injection seeding scheme.

A method for measuring the length of an optical fiber by use of an optical fiber ring laser pulse source is proposed and demonstrated. The key element of the optical fiber ring laser is a gain-switched Fabry-Perot laser diode operated in a self-injection seeding scheme. This method is especially suitable for measuring a medium or long fiber, and a resolution of 0.

Generation of continuously wavelength-tunable optical short pulses by use of two self-seeded Fabry-Perot laser diodes and an optical switch.

Generation of wavelength-tunable optical short pulses by use of two self-seeded Fabry-Perot laser diodes in a parallel configuration is described. The system supports continuous wavelength tuning in a relatively wide range of 42 nm. The side-mode suppression ratio achieved is 35 dB across the entire wavelength tuning range.

[Expression of MUC1 gene and MDR1 gene in non-M3 subtype acute leukemia and their correlations to clinical treatment efficacy].

Background & Objective: Mucin 1 (MUC1) gene is expressed in various tumors, and overexpressed in acute leukemia. This study was to evaluate the expression of MUC1 gene and multidrug-resistance protein-1 (MDR1) gene in non-M3 subtype acute leukemia and their correlations to clinical treatment efficacy.

Methods: The expression of MUC1 and MDR1 genes were measured in 34 patients with non-M3 subtype acute leukemia by reverse transcription-polymerase chain reaction (RT-PCR); their correlations to clinical treatment efficacy were observed.

[Research on TALF expression in Escherichia coli].

The expression of cDNA encoding Tachyleus auti-lipoposaccharide (LPS) factor, which is of interest for use as a potential inhibitor of the common core subunit of Gram-negative bacterial endotoxin. First, the TALF gene was inserted into expression vectors pGEX-4T-2, pET22b and pET28a to construct recombinant expression plasmids. The recombinant plasmids were transformed to E.

Expression of an antimicrobial peptide identified in the male reproductive system of rats.

Bin1b is a beta-defensins-like molecule originally isolated from the rat epididymis. Owing to its bactericidal activity, Bin1b may have therapeutic properties suitable for the treatment of sexually transmitted diseases. The amino terminus of the mature Bin1b peptide contains a conserved myristoylated Gly residue.

Tunable dual-wavelength optical short-pulse generation by use of a fiber Bragg grating and a tunable optical filter in a self-seeding scheme.

A simple self-seeding scheme is developed to generate tunable dual-wavelength optical short pulses in a flexible manner and with an increased wavelength-tuning range. The wavelength selection and tuning are achieved by simultaneous use of a fiber Bragg grating and a tunable optical filter. The side-mode suppression ratio of the output pulses is better than 30 dB over a wavelength-tuning range of 33.

Cloning of anti-lPS factor cDNA from Tachypleus tridentatus, expression in Bombyx mori larvae and its biological activity in vitro.

In this article we report the cloning and expression of a cDNA encoding Tachypleus anti-lipopolysaccharide (LPS) factor, which is of interest for use as a potential inhibitor of the common core subunit of Gram-negative bacterial endotoxins. First, two degenerate primers were designed based on the sequence homology of anti-LPS factors purified from different species of horseshoe crab. The total RNA was extracted from amebocytes of Tachypleus tridentatus.

[Cloning and expression of Tachypleus tridentatus factor C].

Factor C is an endotoxin-sensitive, intracellular serine protease zymogen which initiates the coagulation cascade system in the horseshoe crab hemolymph. The special lipopolysaccharide (LPS) binding activation of FC makes it a potential drug for anti-LPS treatment and has a high commercial value. Based on the sequence of reported FC from Japan horseshoe crab, two pairs of primers were designed.