Development of a DNAzyme-Driven Fluorescent Light-Up Aptasensor for Label-Free Detection of Multiple lncRNAs.

Long noncoding RNAs (lncRNAs) act as the dynamic regulatory molecules that control the expression of genes and affect numerous biological processes, and their dysregulation is associated with tumor progression. Herein, we develop a fluorescent light-up aptasensor to simultaneously measure multiple lncRNAs in living cells and breast tissue samples based on the DNAzyme-mediated cleavage reaction and transcription-driven synthesis of light-up aptamers. When target lncRNAs are present, they can be recognized by template probes to form the active DNAzyme structures, initiating the T4 PNK-catalyzed dephosphorylation-triggered extension reaction to generate double-strand DNAs with the T7 promoter sequences.

Elongation and Ligation-Mediated Differential Coding for Label-Free and Locus-Specific Analysis of 8-Oxo-7,8-dihydroguanine in DNA.

Oxidative DNA damage is closely associated with the occurrence of numerous human diseases and cancers. 8-Oxo-7,8-dihydroguanine (8-oxoG) is the most prevalent form of DNA damage, and it has become not only an oxidative stress biomarker but also a new epigenetic-like biomarker. However, few approaches are available for the locus-specific detection of 8-oxoG because of the low abundance of 8-oxoG damage in DNA and the limited sensitivity of existing assays.

SARM1 participates in axonal degeneration and mitochondrial dysfunction in prion disease.

Prion disease represents a group of fatal neurogenerative diseases in humans and animals that are associated with energy loss, axonal degeneration, and mitochondrial dysfunction. Axonal degeneration is an early hallmark of neurodegeneration and is triggered by SARM1. We found that depletion or dysfunctional mutation of SARM1 protected against NAD loss, axonal degeneration, and mitochondrial functional disorder induced by the neurotoxic peptide PrP.

Complete chloroplast genome of D.G. Zhang, Ying Liu & Q. E. Yang (Asteraceae), a narrow endemic species in Wuling Mountain Region, China.

The first complete chloroplast genome (cp) of D.G. Zhang, Ying Liu & Q.

A Novel Application of Educational Management Information System based on Micro Frontends.

With the launch of the Education Informatization 2.0 action plan by the Ministry of Education, a large number of college information systems have been born in China. Most of these systems are single page web applications (SPA) based on traditional MVC structures.

[Clinical significance of changes of lipid in elderly patients with hepatic schistosomiasis].

Objective: To explore the clinical significance of lipid levels including total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL-C), low density lipoprotein (LDL-C), and apolipoprotein (APOA I and APOB) of elderly patients with hepatic schistosomiasis.

Methods: A total of 280 hospitalized elderly patients with hepatic schistosomiasis (198 cases of chronic liver fibrosis and 82 cases of hepatocirrhosis) were chosen as study objects, and their clinical data were collected and analyzed retrospectively. Meanwhile, the lipid levels between the patients with liver fibrosis and hepatocirrhosis, and those among the patients with A, B, C degrees of Child-pugh grading of liver function were compared.

[Influencing factors in elderly patients with schistosomiasis liver disease combined with gallbladder diseases].

Objective: To understand the status of elderly patients with schistosomiasis liver diseases combined with gallbladder diseases, and explore the influencing factors.

Methods: A total of 280 elderly patients with schistosomiasis liver disease were divided into two groups, 198 cases of chronic liver fibrosis and 82 cases of liver cirrhosis, and the results of their gallbladder ultrasound and liver function examinations were analyzed statistically.

Results: Among the 280 cases, 157 patients were combined with gallbladder diseases (56.

Biological activities of skin secretions of the salamander Tylototriton verrucosus.

Water-soluble skin secretions of salamander Tylototriton verrucosus, first described by Anderson in 1871, were studied for their biological and enzymatic activities. They were found to be toxic to mice with an intraperitoneal LD50 of 11.5 mg/kg.