Application of mesenchymal stromal cells in bone marrow transplantation for sensitized recipients.

Sensitized patients are at high risk for graft rejection during transplantation. It is of interest to investigate the effect of mesenchymal stromal cells (MSCs) in sensitized hematopoietic stem cell transplantation. MSCs were generated from bone marrow cells of BALB/c mice.

[Effects of mesenchymal stem cells on lymphocyte proliferation of sensitized mice in vitro and their action manner].

This study was purposed to investigate the effects of mesenchymal stem cells (MSC) on lymphocyte proliferation of sensitized mice in vitro and their action manner so as to more understand the possible mechanisms of bone marrow-derived MSC action on the engraftment of hematopoietic stem/progenitor cells in sensitized mice. Bone marrow-derived MSC were cultured by adherent culture method, the MSC or culture supernatant were used as immune cells or immunologic factor, the spleen lymphocytes of sensitized mice were used as effector cells. The phytohemagglutinin (PHA) was used to stimulate the lymphocyte proliferation, the MTT method was used to detect the mixed lymphocyte reaction.

[The induction and function of dendritic cells from human cord blood with IFN-alpha and GM-CSF in vitro].

Objective: To probe into the possibility of obtaining dendritic cells (DCs) from human cord blood with rhIFN-alpha and rhGM-CSF.

Methods: The plastic adherent cells (monocyte-rich cells) from cord blood were cultured with rhGM-CSF and rhIFN-alpha for 7 days. The morphological properties of DCs were observed by microscopy, the expression of CD83 and CD86 on DCs were determined by flow cytometry.