Regulating the balance of Th17/Treg cells in gut-lung axis contributed to the therapeutic effect of Houttuynia cordata polysaccharides on H1N1-induced acute lung injury.

Our previous study had demonstrated that oral administration of Houttuynia cordata polysaccharides (HCP) without in vitro antiviral activity ameliorated gut and lung injuries induced by influenza A virus (IAV) in mice. However, as macromolecules, HCP was hard to be absorbed in gastrointestinal tract and had no effect on lung injury when administrated intravenously. The action mechanism of HCP was thus proposed as regulating the gut mucosal-associated lymphoid tissue (GALT).

A new abietane diterpene and anti-complementary constituents from .

Anti-complementary activity-guided fractionation led to the isolation of a new abietane diterpene () and twenty-five known compounds () from the twigs and leaves of . All the compounds were isolated from for the first time. The structure of was assigned by spectroscopic data and X-ray crystallography analysis.

[Suppression to ameloblastoma xenografts of chicken embryo chorioallantoic membrane by tissue inhibitor of metalloproteinases-2].

Objective: To explore the invasiveness of xenografts on chicken embryo chorioallantoic membrane (CAM) after tissue inhibitor of metalloproteinase-2 (TIMP-2) gene transfection.

Methods: Fresh ameloblastoma tissues were minced into 1-2 mm3 and transplanted on the CAM. There were three groups named as control group (Empt), plasma transfection group (Lipo), and TIMP-2 gene transfection group (P).

Cephalometric analysis of craniofacial malformations in newborn mice with cleft palate induced by retinoic acid.

Objective: To determine changes on craniofacial growth morphometrically in newborn mice with cleft palate induced by retinoic acid. DESIGN, SETTING, PARTICIPANTS, INTERVENTIONS: Gestation day 10 or 12 pregnant female C57BL/6N mice were given a single dose of all-trans retinoic acid (atRA) by gastric intubations via oral gavage. Sixty newborn mice with cleft palate (CP), 52 without CP from the experimental group, and 30 without CP from the control group were collected, and lateral cephalograms were taken of all of the mice.

Chronological age estimation of third molar mineralization of Han in southern China.

In order to study the chronology of age of third molar mineralization of Han in southern China, Demirjian staging method was used to determine the stage of four third molars (18, 28, 38, 48) mineralization in 3,100 Han in southern China aged 4.1-26.9 years based on radiological evidence from digital orthopantomograms.

Inhibition of ameloblastoma invasion in vitro and in vivo by inhibitor of metalloproteinase-2 activity.

Background: Ameloblastoma is an odontogenic benign tumor characterized by local invasiveness and most of its local recurrences clinically result from local invasion. This study used matrix metalloproteinase-2 (MMP-2) inhibitor I (MMP-2I) to investigate the role played by MMP-2 activity in the local invasiveness of ameloblastoma.

Methods: The cells and xenografts of ameloblastoma were treated with MMP-2I and treatment group were compared with the control group.

[Establishment of subcutaneous xenotransplanted tumor model of human ameloblastoma in nude mice].

Objective: To establish an subcutaneous xenotransplanted tumor model of human ameloblastoma in nude mice.

Methods: Ameloblastoma cells were absorbed by primary culture, repeat attachment and pancreas proteolytic enzyme were both used to purify them. Then, the purified cells were implanted subcutaneously into the nude mice.

[The construction and expression of PcDNA3.1(+)/GFP-TIMP-2 in human ameloblastoma cell].

Objective: To construct the eukaryotic expression vector of TIMP-2 gene and to explore its expression in human ameloblastoma cell in vitro.

Methods: The aimed gene fragment was obtained by RT-PCR. And then, molecmicrolar cloning technology and enzyme digestion were used to connect the gene with the plasmid PcDNA3.