Publications by authors named "Dong-Hyeon Yeom"
Article Synopsis
- Successful dog cloning hinges on having enough matured oocytes for embryo reconstruction, with accurate ovulation prediction being crucial for collecting these oocytes.
- This study compared two systems, radioimmunoassay (RIA) and enzyme-linked fluorescence assay (ELFA), for measuring progesterone levels in estrus bitches to determine the best method for ovulation prediction.
- Results showed that the ELFA system not only provided a higher rate of matured oocytes (65.19% vs. 55.47% for RIA) but also successfully led to the birth of cloned puppies, indicating its viability for this purpose.
The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.
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