USP1 promotes pancreatic cancer progression and autophagy by deubiquitinating ATG14.

Pancreatic ductal adenocarcinoma (PDAC) is characterized by extremely poor prognosis, high mortality, and limited therapeutic strategy. Autophagy is hyperactivated in PDAC, and targeting autophagy is emerging as a promising therapeutic strategy. The dysfunction of deubiquitinase ubiquitin-specific peptidase 1 (USP1) results in tumorigenesis and chemotherapy resistance.

A bright cyan fluorescence calcium indicator for mitochondrial calcium with minimal interference from physiological pH fluctuations.

Genetically Encoded Calcium (Ca) indicators (GECIs) are indispensable tools for dissecting intracellular Ca signaling and monitoring cellular activities. Mitochondrion acts as a Ca sink and a central player for maintaining Ca homeostasis. Accurately monitoring Ca transients within the mitochondrial matrix that undergo constant pH fluctuations is challenging, as signals of most currently available GECIs suffer from artifacts induced by physiological pH variations.

Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags.

Rapid, direct and sensitive detection of foodborne bacteria in complex samples is still challenging. Here, we reported a universal surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) for highly sensitive detection of foodborne bacteria in food and environmental samples using wheat germ agglutinin (WGA)-modified FeO@Au (Au@MNP-WGA) nanotags. The Au@MNP-WGA tag with numerous intraparticle hotspots was integrated into the LFA system for the first time, which can not only greatly improve the detection sensitivity through the dual amplification effect of magnetic enrichment and SERS enhancement but also achieve the broad-spectrum capture of multiple bacteria.

7-Methoxyisoflavone ameliorates atopic dermatitis symptoms by regulating multiple signaling pathways and reducing chemokine production.

7-Met, a derivative of soybean isoflavone, is a natural flavonoid compound that has been reported to have multiple signaling pathways regulation effects. This study investigated the therapeutic effects of 7-Met on mice with atopic dermatitis induced by fluorescein isothiocyanate (FITC), or oxazolone (OXZ). 7-Met ameliorated FITC or OXZ-induced atopic dermatitis symptoms by decreasing ear thickness, spleen index, mast cell activation, neutrophil infiltration and serum IgE levels in female BALB/c mice.

Rapid SERS identification of methicillin-susceptible and methicillin-resistant aptamer recognition and deep learning.

Here, we report a label-free surface-enhanced Raman scattering (SERS) method for the rapid and accurate identification of methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) based on aptamer-guided AgNP enhancement and convolutional neural network (CNN) classification. Sixty clinical isolates of (), comprising 30 strains of MSSA and 30 strains of MRSA were used to build the CNN classification model. The developed method exhibited 100% identification accuracy for MSSA and MRSA, and is thus a promising tool for the rapid detection of drug-sensitive and drug-resistant bacterial strains.

Optimization and characterization of biosurfactant produced by indigenous isolated from a low permeability reservoir for application in MEOR.

Biosurfactants are expected to be a key factor for microbial enhanced oil recovery (MEOR). In this study, we described the novel biosurfactant-producing strain YZ-2 isolated from a low permeability oil reservoir. We purified and characterized the biosurfactants produced by this YZ-2 strain thin-layer chromatography and MALDI-TOF-MS, revealing them to be fengycins.

Surface Stability and Morphology of Calcium Phosphate Tuned by pH Values and Lactic Acid Additives: Theoretical and Experimental Study.

The ubiquitous mineralization of calcium phosphate (CaP) facilitates biological organisms to produce hierarchically structured minerals. The coordination number and strength of Ca ions with phosphate species, oxygen-containing additives, and solvent molecules played a crucial role in tuning nucleation processes and the surface stability of CaP under the simulated body fluid (SBF) or aqueous solutions upon the addition of oligomeric lactic acid (LAC, = 1, 8) and changing pH values. As revealed by ab initio molecular dynamics (AIMD), density functional theory (DFT), and molecular dynamics (MD) simulations as well as high-throughput experimentation (HTE), the binding of LAC molecules with Ca ions and phosphate species could stabilize both the pre-nucleation clusters and brushite (DCPD, CaHPO·2HO) surface through intermolecular electrostatic and hydrogen bonding interactions.

Evaluation of Schott in anti-cancerous properties with proximity extension assays.

Background: Schott (called as Xiangshayu in Chinese) is an excellent local cultivar of the genus polymorpha in Jiangsu Province, China.

Objective: In the present study, we have performed a comparative study before and after dietary consumption with Schott to evaluate its anti-cancerous properties.

Design: Forty-two healthy volunteers were recruited, and dietary consumption with 200 g of tap water cooked Schott daily was conducted for 1 month.

Self-Immolative Dye-Doped Polymeric Probe for Precisely Imaging Hydroxyl Radicals by Avoiding Leakage.

For sensing low abundance of biomarkers, utilizing nanocarriers to load dyes is an efficient method to amplify the detected signal. However, the non-specific leak of the internal dyes in this approach is accompanied by false positive signals, resulting in inaccurate signal acquirement. To address this issue, in this work, we reported a novel signal amplification strategy with dye as a scaffold to construct a self-immolative dye-doped polymeric probe (SDPP).

IL-21 Stimulates the expression and activation of cell cycle regulators and promotes cell proliferation in EBV-positive diffuse large B cell lymphoma.

The clinical features of EBV-positive diffuse large B cell lymphoma (DLBCL) indicate a poorer prognosis than EBV-negative DLBCL. Currently, there is no efficacious drug for EBV-positive DLBCL. The cytokine interleukin-21 (IL-21) has been reported to be pro-apoptotic in DLBCL cell lines and is being explored as a new therapeutic strategy for this type of lymphomas.

Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3).

Background: Porcine circovirus type 3 (PCV3) has been an emerging porcine virus spread around the world. The conserved DNA sequence of PCV3 enabled good performance in molecular biological assays.

Result: In this study, we developed a real-time fluorescence PCR assay for the detection of PCV3.

Tracking alterations of alkyl side chains of N species in heavy crude oil after anaerobic biodegradation with negative-ion electrospray ionization coupled with high-field Fourier transform ion cyclotron resonance mass spectrometry.

Rationale: Heteroatomic compounds are relatively abundant and believed to be bio-resistant in heavy crude oils. However, few studies have focused on the biodegradation of these heteroatomic compounds.

Methods: Heteroatoms, especially N species, in a blank crude oil and in three treated oils co-incubated with anaerobic sulfate-reducing bacteria, nitrate-reducing bacteria and fermentative consortia cultures were detected using negative-ion electrospray ionization coupled with high-field Fourier transform ion cyclotron resonance mass spectrometry.

Versatile Spectral and Lifetime Multiplexing Nanoplatform with Excitation Orthogonalized Upconversion Luminescence.

Optical encoding together with color multiplexing benefits on-site detection, and enriching the components with narrow emissions from lanthanide could greatly increase the coding density. Here, we show a typical example to combine emission color and lifetime that are simultaneously integrated in a single lanthanide nanoparticle. With the multicompartment core/shell structure, the nanoparticles can activate different emitting pathways under varied excitation.