Basic Considerations for Data Pooling Strategy in Multi-Regional Clinical Trials (MRCTs).

The National Medical Products Administration of China has been implementing ICH E17, which describes the general principles for planning and designing of multi-regional clinical trials (MRCTs), yet there are several ambiguities in the execution and conduct remains in China or East Asia. In specific, pooling strategy, effect modifiers (EMs), statistical analysis, sample size allocation and their impact in alignment with global trial remains a challenge. In this paper, we explore on the criteria mentioned above under the context of China.

Encapsulated FeC boosted electrocatalytic performance for oxygen reduction reaction of N-doped carbon nanotube.

The shortcomings of precious metal based catalysts have limited the development of novel energies. So, developing low-cost and high performance transition metal based catalysts is one of the most feasible way to substitute the precious metal based catalysts. In all of the developed catalysts for oxygen reduction reactions (ORR), the iron-based nitrogen doped carbon nanotube (N-CNT) show great promise.

p62 Binding to Protein Kinase C Regulates HIV-1 gp120 V3 Loop Induced Microglial Inflammation.

The main pathogenic mechanism of HIV-associated neurocognitive disorders (HAND) is neuronal apoptosis induced by inflammatory mediators, in which microglial inflammation plays a crucial role. However, the exact pathogenic mechanism remains unclear. Previous studies have shown that the HIV-1 gp120 V3 loop can trigger inflammation in CHME-5 microglia.

Switch-Type Electrochemiluminescence Aptasensor for AFB1 Detection Based on CoS Quantum Dots Encapsulated in Co-LDH and a Ferrocene Quencher.

Among the various aflatoxin B1 (AFB1) assays, performing accurate detection is difficult because false positives and false negatives are frequent due to limited sensitivity, expensive equipment, or inadequate pretreatment during operation. Here, an "off-on" switch-type electrochemiluminescence (ECL) aptasensor armed with cobalt-sulfur quantum dots was encapsulated in hollow cobalt-layered double hydroxide nanocages as an enhanced luminescent probe (Co-LDH@QDs), and a ferrocene-modified aptamer (Fc-APT) was used as a luminescent quencher. In general, when Fc-APT was hybridized with complementary DNA modified with a DNA nanotetrahedron, electron transfer between ferrocene and Co-LDH@QDs was facilitated, leading to efficient quenching of the ECL intensity into an "off" state in the absence of AFB1.

Multi-perspective kinetic analysis of plastic blends under thermogravimetric pyrolysis conditions.

Plastic blends were co-pyrolyzed under non-isothermal conditions in a thermogravimetric (TG) analyzer. The co-pyrolysis characteristics and kinetic triplet, i.e.

Prognostic analysis of elective neck dissection in cT3/T4a/T4bN0 maxillary sinus squamous cell carcinoma.

Purpose: This study aimed to examine the effect of elective neck dissection (END) on the prognosis of patients with cT3/T4N0 maxillary sinus squamous cell carcinoma (MSSCC).

Methods: Data were obtained from the SEER database. Patients with cT3/T4a/T4bN0 MSSCC were included in the study and divided into two groups: those who received END treatment and those who did not.

Microfluidics-based automatic immunofluorescence staining for single-molecule localization microscopy.

Full automation of single-molecule localization microscopy (SMLM) is crucial for large-scale and high-throughput cellular imaging. It is well-known that SMLM typically consists of three major steps: immunofluorescence (IF) staining, optical imaging, and image processing. Currently, automation in optical imaging and image processing is almost complete; however, the automation of IF staining has been slow to advance, probably due to its complicated experimental operations.

Efficacy of neoadjuvant therapy and lymph node dissection in advanced gallbladder cancer without distant metastases: a SEER database analysis.

Purpose: To investigate the effectiveness of neoadjuvant therapy and lymph node dissection(LND) on overall survival (OS) in patients with stage III/IV gallbladder cancer without distant metastases.

Methods: Data from 101 patients who received neoadjuvant therapy followed by surgery combined with adjuvant chemotherapy, and 1412 patients who received direct surgical treatment followed by adjuvant chemotherapy, were collected from the SEER database from 2004 to 2020. Patients were divided into group A (neoadjuvant therapy) and group B (direct surgery) based on the treatment modality.

Shear wave elastography based analysis of changes in fascial and muscle stiffness in patients with chronic non-specific low back pain.

Background: The quantitative assessment of individual muscle and fascial stiffness in patients with low back pain remains a challenge. This study aimed to compare the stiffness of the thoracolumbar fascia (TLF), erector spinae (ES), and multifidus (MF) in patients with and without chronic non-specific low back pain (CNLBP) using shear wave elastography (SWE). It also sought to explore the relationship between muscle and fascial stiffness and the levels of pain and dysfunction in patients with CNLBP.

Bias control for optical single-sideband transmitter based on single IQ modulator.

The optical in-phase and quadrature modulator (IQM) is typically utilized as an optical single-sideband (SSB) transmitter for its simple structure and low cost. It requires the modulator biases to be slightly offset for generating the optical carrier. However, this poses significant challenges in the bias control circuits, as most off-the-shelf automatic bias control (ABC) modules are designed to stabilize the IQM at its carrier suppression point for coherent optical communication.

Compatible metasurface for ultra-wideband radar and switchable infrared stealth.

In response to the rapid advancements in radar detection technology and the widespread deployment of infrared sensors, single-function stealth materials are increasingly challenged to meet the sophisticated demands of concealment within complex electromagnetic environments. As a result, there is a pressing need for research into metamaterial structures that can simultaneously deliver ultra-wideband radar stealth and controllable infrared invisibility. Here, a novel metamaterial structure was proposed and realized, comprising vertically integrated infrared stealth and radar stealth layers, with the aim of accomplishing both ultra-wideband radar stealth and controlled infrared invisibility.

CRISPR-based molecule-regulatory expression platform for specific immunotherapy of cancer.

Introduction: Cancer is still a major challenge of human health. The abnormality of intracellular cancer-related signal pathways is an important mechanism for the occurrence of cancer.

Methods: We used a molecular-senor to act on the endogenous signal molecules within the cell to redirect the abnormal signal flows in the cell to treat cancer.

Novel phase transfer catalysis coupled with bifunctional oxidation for enhanced remediation of groundwater polluted with multiple NAPL: Performance and mechanisms.

Structural differences among non-aqueous phase liquids (NAPLs) result in varying oxidation rates, limiting mass transfer between NAPLs and oxidants and seriously impairing the effectiveness of remediation via traditional in-situ chemical oxidation. To tackle this challenge, a novel approach is proposed for remediating multi-NAPL-polluted groundwater that leverages phase transfer catalysis (PTC) to enhance heterogeneous mass transfer by transferring oxidants from groundwater to NAPLs. Meanwhile, "oxidation-in-situ activation" is achieved through bifunctional oxidation using permanganate and peroxymonosulfate (PP).

The association of aromatic amino acids with coronary artery disease and major adverse cardiovascular events in a Chinese population.

This study aimed to evaluate the relationship between aromatic amino acids (AAAs), - phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) - and coronary artery disease (CAD) in a prospective study involving 2970 participants undergoing coronary angiography at Beijing Hospital. Serum levels of Phe, Tyr and Trp were analysed. The cross-sectional data revealed that serum Tyr and Trp levels were significantly and inversely associated with CAD.

ADSCs encapsulated in Gelatin methacrylate substrate promotes the repair of peripheral nerve injury by SIRT6/PGC-1α pathway.

Peripheral nerve injury is a prevalent disease but the spontaneous recovery of nerve function is protracted and incomplete. Given the damaging of stem cells and fragile of intra-neural structures in the course of stem cell transplantation, our study tried to investigate whether encapsulating adipose derived mesenchymal stem cells (ADSCs) with GelMA could achieve better repair in peripheral nerve injury. PC-12 cells were cultured on the surface of GelMA encapsulating ADSCs and 3D co-culture system was constructed.

Synergistic approach of GCW-ISTR for enhanced remediation of semi-volatile organic contaminants in groundwater: Modeling and experimental validation.

The poor remediation performance of groundwater circulation well (GCW) on semi-volatile organic contaminants (such as aniline) has severely limited its practical application. To address the challenges posed by the low volatility and solubility of these contaminants, an innovative integration of GCW with in-situ thermal remediation (ISTR) was proposed to create a thermal enhanced circulation well (GCW-ISTR) in this study. Laboratory experiments and model simulations were performed to evaluate the heat transfer and enhanced remediation effect by GCW-ISTR.

Density modification of DNAPL using self-demulsifying colloidal biliquid aphron: Kinetics, mechanisms and influencing factors.

Density-modification remediation of dense nonaqueous phase liquid (DNAPL) using colloidal biliquid aphron (CBLA) is an efficient means of enhancing flushing and avoiding the risk of downward migration of DNAPL. However, the use of demulsifier is currently necessary for CBLA to achieve density modification. This leads to issues such as low modification efficiency and the risk of secondary contamination.

OTULIN's influence on neuroinflammation and pain modulation in trigeminal neuralgia.

Introduction: Trigeminal neuralgia (TN), marked by chronic pain from neural damage, is closely associated with inflammation. The role of OTULIN, a key regulator in inflammation and autophagy, is not fully understood in TN. The regulatory mechanism of OTULIN, a key protein involved in modulating inflammatory responses and autophagy processes, remains incompletely elucidated, particularly in the context of TN and neuroinflammation.

Pancreatic head carcinoma derived from the dorsal pancreas is more likely to metastasize early than from the ventral pancreas through microvascular invasion.

The development of the pancreatic head originates from the fusion of the ventral and dorsal pancreatic primordia during embryonic development. Theoretically, the origin of pancreatic head cancer also exists from the ventral pancreas and the dorsal pancreas. Among 49 patients with pancreatic head cancer, pancreatic head cancer was divided into pancreatic head cancer originating from the ventral (PHCv) or dorsal pancreas (PHCd) through imaging and pathological classification.

Boost Infiltration and Activity of T Cells via Inhibiting Ecto-5'-nucleotidase (CD73) Immune Checkpoint to Enhance Glioblastoma Immunotherapy.

The currently available immune checkpoint therapy shows a disappointing therapeutic efficacy for glioblastoma multiforme (GBM), and it is of great importance to discover better immune checkpoints and develop innovative targeting strategies. The discovered metabolic immune checkpoint ecto-5-nucleotidase (CD73) in a tumor contributes to its immune evasion due to the dysregulation of extracellular adenosine (ADO), which significantly inhibits the function of antitumor T cells and increases the activity of immunosuppressive cells. Herein, we drastically inhibit the expression of CD73 to reduce the production of ADO by using versatile Au@CuSe nanoparticles (ACS NPs).

Molecular heterogeneity and treatment outcome of EGFR exon 20 insertion mutations in Chinese patients with advanced non-small cell lung cancer: insights from a large-scale real-world study.

Introduction: This retrospective study aimed to investigate treatment patterns and outcomes in patients with NSCLC harboring EGFR20ins in China. EGFR20ins mutations are associated with poor responses to EGFR-TKIs, and limited real-world data exist regarding the efficacy of various treatment modalities.

Methods: In this retrospective, single-center study, treatment outcomes, including PFS and ORR, were evaluated for different treatment regimens based on imaging assessments.

Demographic and zoological drivers of infectome diversity in companion cats with ascites.

Unlabelled: Cats () have become an integral part of many households. However, our understanding of the full spectrum of pathogens affecting cats (referred to as the infectome) is limited, mainly due to the inadequacy of commonly used diagnostic tools in capturing the complete diversity of potential pathogens and the prevalence of pathogen co-infections. In this study, we employed a meta-transcriptomic approach to simultaneously characterize the infectome contributing to different disease syndromes and to investigate spatial, demographic, and ecological factors influencing pathogen diversity and community composition in a cohort of 27 hospitalized cats and seven stray cats.