Adding mixed probiotic to a low-crude-protein diet: Effects on production efficiency, nutrient retention, faecal gas discharge, faecal score and meat quality of finishing pigs.

This study examined the impact of mixed probiotic inclusion in a reduced crude protein (CP) diet on production performance, nutrient retention, gas emissions, faecal score and meat quality of finishing pigs. In total, 150 pigs (body weight [BW] of 49.9 ± 2.

CPGminer: An Interactive Dashboard to Explore the Genomic Features and Taxonomy of Complete Prokaryotic Genomes.

Prokaryotes, the earliest forms of life on Earth, play crucial roles in global biogeochemical processes in virtually all ecosystems. The ever-increasing amount of prokaryotic genome sequencing data provides a wealth of information to examine fundamental and applied questions through systematic genome comparison. Genomic features, such as genome size and GC content, and taxonomy-centric genomic features of complete prokaryotic genomes (CPGs) are crucial for various fields of microbial research and education, yet they are often overlooked.

Anti-Inflammatory Efficacy of Human-Derived on Periodontopathogen-Induced Inflammation.

is a beneficial bacterium in oral cavity, and some strains of this bacterium are known to be probiotics. The purpose of this study was to investigate the anti-inflammatory effect and mechanism of G7 lipoteichoic acid (LTA) on lipopolysaccharide (LPS) and LTA of periodontopathogens. The surface molecules of G7 was extracted, and single- or co-treated on human monocytic cells with LPS and LTA of periodontopathogens.

Effective microbial molecular diagnosis of periodontitis-related pathogen Porphyromonas gingivalis from salivary samples using rgpA gene.

Importance of accurate molecular diagnosis and quantification of particular disease-related pathogenic microorganisms is highlighted as an introductory step to prevent and care for diseases. In this study, we designed a primer/probe set for quantitative real-time polymerase chain reaction (qRT-PCR) targeting rgpA gene, known as the specific virulence factor of periodontitis-related pathogenic bacteria 'Porphyromonas gingivalis', and evaluated its diagnostic efficiency by detecting and quantifying relative bacterial load of P. gingivalis within saliva samples collected from clinical subjects.

Probiotic mixture (Bacillus subtilis and Bacillus licheniformis) a potential in-feed additive to improve broiler production efficiency, nutrient digestibility, caecal microflora, meat quality and to diminish hazardous odour emission.

This research aimed to assess the impact of probiotic supplementation in the broiler diet on growth performance, nutrient utilization, noxious gas emissions, excreta micromiota and meat quality. One thousand six hundred and twenty male Ross 380 broilers (one-day-old, body weight, 42 ± 0.5 g and 5-week trial) were arbitrarily chosen and assigned to three nutritive treatments (basal diet and basal diet included with 0.

Dietary Inclusion of Blood Plasma with Yeast () Supplementation Enhanced the Growth Performance, Nutrient Digestibility, Lactobacillus Count, and Reduced Gas Emissions in Weaning Pigs.

This experiment was performed to examine the hypothesis that blood plasma (BP) with yeast ( supplement in the diet of weaning pigs could provoke the growth performance, nutrient digestibility, fecal microbial, and reduce harmful gas excretion. A total of one hundred and eighty healthy piglets were taken and assigned (complete random blocks) to three dietary treatments as: Phase 1: Treatment (TRT) 1-6% BP; TRT 2-3% BP + 3% yeast; TRT 3-6% yeast. Phase 2: TRT 1-3%; BP.

Characteristics of Treponema denticola lipooligosaccharide in presence of hemin and quorum-sensing molecule.

Objectives: The study aimed to examine the diverse bioactivity of lipooligosaccharide extracted from T. denticola cultured in the presence of hemin and quorum-sensing inhibitor.

Design: T.

CYPminer: an automated cytochrome P450 identification, classification, and data analysis tool for genome data sets across kingdoms.

Background: Cytochrome P450 monooxygenases (termed CYPs or P450s) are hemoproteins ubiquitously found across all kingdoms, playing a central role in intracellular metabolism, especially in metabolism of drugs and xenobiotics. The explosive growth of genome sequencing brings a new set of challenges and issues for researchers, such as a systematic investigation of CYPs across all kingdoms in terms of identification, classification, and pan-CYPome analyses. Such investigation requires an automated tool that can handle an enormous amount of sequencing data in a timely manner.

Effect of dietary supplemental medium chain fatty acids instead of antibiotics on the growth performance, digestibility and blood profiles in growing pigs.

Antibiotics have great functions in farm animal. However, the harm of antibiotics can't be ignored. The effects of medium-chain fatty acids (MCFAs) supplementation to basal diet instead of antibiotics (CSP, Chlortetracycline, sulphonamide dimethazine and procaine penicillin, 1:1:1) on growth performance, nutrient digestibility and blood profile in growing pigs were studied.

Antimicrobial activity of NO-releasing compounds against periodontal pathogens.

This study describes the successful synthesis of nitric oxide (NO)-releasing compounds with biodegradable and injectable properties and demonstrates that the kinetics of NO release vary according to the type of NO donor. The antimicrobial activity of NO-releasing compounds against three common periodontal pathogens, i.e.

Inhibitory effect of Lactococcus lactis on the bioactivity of periodontopathogens.

Lactococcus lactis is a probiotic bacterium that produces various bacteriocins. Periodontopathogens induce inflammation and halitosis through the actions of lipopolysaccharide (LPS) and trypsin-like enzymes. The purpose of this study was to investigate the inhibitory effects of L.

Comparative functional pan-genome analyses to build connections between genomic dynamics and phenotypic evolution in polycyclic aromatic hydrocarbon metabolism in the genus Mycobacterium.

Background: The bacterial genus Mycobacterium is of great interest in the medical and biotechnological fields. Despite a flood of genome sequencing and functional genomics data, significant gaps in knowledge between genome and phenome seriously hinder efforts toward the treatment of mycobacterial diseases and practical biotechnological applications. In this study, we propose the use of systematic, comparative functional pan-genomic analysis to build connections between genomic dynamics and phenotypic evolution in polycyclic aromatic hydrocarbon (PAH) metabolism in the genus Mycobacterium.

Effects of Streptococcus thermophilus on volatile sulfur compounds produced by Porphyromonas gingivalis.

Halitosis as oral malodour is an unpleasant odour caused by volatile sulfur compounds (VSCs). VSCs are produced primarily by anaerobic bacteria that abundantly produce proteinase as trypsin-like enzyme. General therapies, such as mouthwash and plaque control, do not provide a continuous effect on oral halitosis.

Evaluation of stability changes in magnesium-incorporated titanium implants in the early healing period.

The aim of the present study was to determine the bone response to magnesium (Mg) ion-incorporated titanium (Ti) implants during the early healing period. A total of 114 patients (69 men and 45 women aged between 29 and 71 years) participated in the study. Overall, one hundred thirty-three 10-mm-long fixtures were installed, composed of 65 Mg ion-incorporated fixtures in the test group (55 participants; 30 males and 25 females) and 68 resorbable blast media (RBM) surface fixtures in the control group (59 participants; 39 males and 20 females).

Antibacterial and neutralizing effect of human β-defensins on Enterococcus faecalis and Enterococcus faecalis lipoteichoic acid.

Introduction: Enterococcus faecalis is frequently found in the root canal of teeth, is a major microorganism of endodontic therapy failure, and is associated with chronic apical periodontitis. Human β-defensins (HBDs) are known to play critical roles in defending the host against infectious microbes and producing dental pulp in healthy and patients. The purpose of the present study was to investigate the bactericidal and neutralizing effects of HBDs on E.

Aerosol deposition of hydroxyapatite and 4-hexylresorcinol coatings on titanium alloys for dental implants.

Purpose: Aerosol deposition is a newly developed technique, and it can deliver the drug from a hydroxyapatite (HA)-coated surface. 4-Hexylresorcinol (4-HR) is a well-known antiseptic. The influence of the 4-HR component of HA coatings on titanium surfaces was studied in vitro and in vivo.

Polycyclic aromatic hydrocarbon metabolic network in Mycobacterium vanbaalenii PYR-1.

This study investigated a metabolic network (MN) from Mycobacterium vanbaalenii PYR-1 for polycyclic aromatic hydrocarbons (PAHs) from the perspective of structure, behavior, and evolution, in which multilayer omics data are integrated. Initially, we utilized a high-throughput proteomic analysis to assess the protein expression response of M. vanbaalenii PYR-1 to seven different aromatic compounds.

Restoration of peri-implant defects in immediate implant installations by Choukroun platelet-rich fibrin and silk fibroin powder combination graft.

Objective: The objective of this study was to determine the capability of silk fibroin powder as a biomaterial template for the restoration of peri-implant defects when mixed with Choukroun platelet-rich fibrin (PRF) in vivo.

Study Design: Ten New Zealand white rabbits were used for this study. Using a trephine bur (diameter 7.

Development of strain-specific PCR primers for the identification of Fusobacterium nucleatum subsp. fusiforme ATCC 51190(T) and subsp. vincentii ATCC 49256(T).

The objective of this study was to develop the strain-specific PCR primers for Fusobacterium nucleatum subsp. fusiforme ATCC 51190(T) and F. nucleatum subsp.

ClassRHO: a platform for classification of bacterial rieske non-heme iron ring-hydroxylating oxygenases.

We have developed an easy-to-use multiplatform classification tool, ClassRHO, which facilitates classification and comparison of bacterial Rieske non-heme iron aromatic ring-hydroxylating oxygenases (RHOs). Visualization and analysis can be generated on-the-fly by entering or uploading RHO query sequences. Pre-computed classifications were implemented for 42 standard RHO sequences.

A new classification system for bacterial Rieske non-heme iron aromatic ring-hydroxylating oxygenases.

Background: Rieske non-heme iron aromatic ring-hydroxylating oxygenases (RHOs) are multi-component enzyme systems that are remarkably diverse in bacteria isolated from diverse habitats. Since the first classification in 1990, there has been a need to devise a new classification scheme for these enzymes because many RHOs have been discovered, which do not belong to any group in the previous classification. Here, we present a scheme for classification of RHOs reflecting new sequence information and interactions between RHO enzyme components.

Effects of mushroom, Pleurotus eryngii, extracts on bone metabolism.

We performed the present study to investigate whether Pleurotus eryngii extracts (PEX) play a role in bone metabolism. PEX treatment showed increase in the alkaline phosphatase activity of the osteoblasts and in the osteocalcin mRNA expression from primary osteoblasts. PEX also increased the expression of the Runx2 gene, and the secretion of osteoprotegerin from the osteoblasts showed marked increases after treatment with PEX.

Suppressive effect of AIF, a water extract from three herbs, on collagen-induced arthritis in mice.

AIF has been formulated using three herbs known to have anti-inflammatory and anti-osteolytic effects. In this study, the potential therapeutic effects of AIF for rheumatoid arthritis were assessed in vitro and in vivo. The effects of AIF on the inflammation (TNF-alpha, IL-1, iNO), cartilage protection (MMP-13), and selective killing of activated T cells were examined, in vitro.