A neoteric smartphone-assisted colorimetric and fluorometric dual-mode probe based on anthraquinone derived Schiff base for wide-range pH and Cu/S determination in bioimaging.

The fluctuation of ion content has an important effect on human health and ecological environment. Herein, a novel colorimetric and fluorescent dual mode probe (DMHB) has been designed via the imine bond bridging of anthraquinone derivative dye and salicyl hydrazide. The DMHB displays significance wide-range pH dependent spectral behavior (pKa 5.

Study of association between embryo growth arrest (EGA) and atmospheric fine particulate matter pollution (PM) and spatial metabolomics of villi derived from pregnant women.

Fine particulate matter (PM) is related to embryo growth arrest (EGA). In this study, we collected demographic information from EGA cases and early pregnancy controls in Taiyuan, China, between 2022 and 2023 and obtained villi and serum samples from these participants. We employed multilevel mixed-effects logistic regression models to estimate the odds ratios (ORs).

Chronic Real-Ambient PM Exposure Exacerbates Cardiovascular Risk via Amplifying Liver Injury in Mice Fed with a High-Fat and High-Cholesterol Diet.

Epidemiology has associated fine particulate matter (PM) exposure with an increased cardiovascular risk. However, the underlying mechanism, particularly from the liver perspective, remains unclear. Here, the influence of chronic PM exposure on cardiovascular risk in mice fed a high-fat and high-cholesterol diet (HFCD) was studied by using a real-world PM exposure system.

Simple strategy for dual-responsive ratio electrochemical-colorimetric detection of nitrite in food and environment.

A dual-responsive ratio electrochemical-colorimetric method for nitrite (NO) is established based on the combination of nanoenzyme (MnO) catalysis with diazotization reactions. The MnO can oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) into blue TMBox. The NO induces the diazotization reaction of TMBox, leading to a decrease of  the signal at 652 nm and the generation of a new signal from diazotized TMBox at 445 nm.

Fluorescence and colorimetric dual-mode sensing of copper ions and fingerprint visualization by benzimidazole derivatives.

In this paper, the molecular fluorescence probe H containing an imidazole structure was designed and synthesized by forming a ring between two amino groups and one aldehyde group. The synthesized probe H exhibits a Stokes shift of 144 nm with fluorescence emission at 555 nm and excitation at 411 nm. The fluorescence of probe H was quenched by the addition of Cu and accompanied a red-shift of ultraviolet-visible (UV-Vis) absorption spectrum.

Rational design of carbon dot nanozymes for ratiometric dual-signal and smartphone-assisted visual detection of nitrite in food matrices.

Developing reliable nitrite (NO) sensors is essential for food safety and reducing health risks from NO exposure. In this study, we strategically designed nitrogen-doped carbon dot (N-CD) nanozymes to establish an accessible dual-signal ratiometric sensing system for detecting NO in food matrices. This system utilizes the photoluminescence and enzyme-like properties of N-CD nanozymes combined with NO-triggered diazotization reactions of substrates such as o-phenylenediamine (OPD) or 3,3',5,5'-tetramethylbenzidine (TMB).

A turn-on anthraquinone-derived colorimetric and fluorometric dual-mode probe for highly selective Hg determination and bioimaging in living organisms.

Mercury ion (Hg) is considered a harmful neurotoxin, and real-time monitoring of Hg concentrations in environmental and biological samples is critical. Fluorescent probes are a rapidly emerging visualization tool owing to their simple design and good selectivity. Herein, a novel fluorescence (FL) probe 2-(4-((6-((quinolin-8-yloxy)methyl)pyridin-2-yl)methyl)piperazin-1-yl)anthracene-9,10-dione (QPPA) is designed using piperazine as a linker between the anthraquinone group, which serves as a fluorophore, and NO as the Hg ligand.

Observation of polarity changes in Sjogren's syndrome mice using a targeting lysosomes and ratiometric fluorescent probe.

Utilizing non-invasive, real-time dynamic imaging and high-resolution detection tools to track polarity changes in Sjögren's syndrome (SS) contributes to a better understanding of the disease progression. Herein, a ratiometric polarity-sensitive fluorescent probe (DIM) was designed and synthesized, DIM consisted of dicyanoisophorone as the fluorophore and morpholine moiety as lysosome targeting. DIM showed a ratiometric response to polarity and high selectivity (unaffected by viscosity, pH, ROS, RNS, etc.

Versatile plant genome engineering using anti-CRISPR-Cas12a systems.

CRISPR-Cas12a genome engineering systems have been widely used in plant research and crop breeding. To date, the performance and use of anti-CRISPR-Cas12a systems have not been fully established in plants. Here, we conduct in silico analysis to identify putative anti-CRISPR systems for Cas12a.

Long-term tracking of lysosomal dynamics with highly stable fluorescent probe.

Monitoring lysosomal dynamics in real-time, especially in vivo, poses significant challenges due to the complex and dynamic nature of cellular environments. It is extremely important to construct fluorescent probes with high stability for imaging lysosomes to minimize interference from other cellular components, in order to ensure prolonged imaging. A fluorescent probe (PDB) has been proposed for targeting lysosomes, which was less affected to changes in the cellular microenvironment (such as pH, viscosity and polarity).

Facile synthesis of long-wavelength emission carbon dots for hypochlorite sensing and intracellular pH imaging.

Hypochlorite (ClO), a typical reactive oxygen species, plays an irreplaceable roles in various biological processes. In this work, long-wavelength emission carbon dots (LW-CDs) were fabricated through one-step hydrothermal method by using l-cysteine (cys) and neutral red (NR) as precursors for monitoring of hypochlorite and intracellular pH. Characterizations of as-prepared LW-CDs showed that they had excellent water solubility, high optical stability and sensitive response behavior.

Multifunctional magnetic tags with photocatalytic and enzyme-mimicking properties for constructing a sensitive dual-readout ELISA.

ELISA has become the gold standard for detecting harmful substances due to its specific antibody recognition and sensitive enzyme-catalyzed reactions. In this study, multifunctional magnetic Prussian blue nanolabels (MPBNs) were synthesized using a simple gentle two-step method to achieve a dual-readout mode. The MPBNs provide a sensitive colorimetric signal by efficiently catalyzing the oxidation of TMB and exhibit prominent photocatalytic degradation activity towards Rhodamine B (RhB).

Mitochondria-Targetable Near-Infrared Fluorescent Probe for Visualization of Hydrogen Peroxide in Lung Injury, Liver Injury, and Tumor Models.

Hydrogen peroxide (HO) overexpressed in mitochondria has been regarded as a key biomarker in the pathological processes of various diseases. However, there is currently a lack of suitable mitochondria-targetable near-infrared (NIR) probes for the visualization of HO in multiple diseases, such as PM exposure-induced lung injury, hepatic ischemia-reperfusion injury (HIRI), nonalcoholic fatty liver (NAFL), hepatic fibrosis (HF), and malignant tumor tissues containing clinical cancer patient samples. Herein, we conceived a novel NIR fluorescent probe () by introducing pentafluorobenzenesulfonyl as a HO sensing unit into the NIR hemicyanine platform.

Ultrasensitive monitoring of PCB77 in environmental samples using a visible-driven photoelectrochemical sensing platform coupling with exonuclease I assisted in target recycling.

Due to the urgent need for detecting trace amounts of 3,3',4,4'-tetrachlorobiphenyl (PCB77) in the environment, we have developed an efficient and visible-driven photoelectrochemical (PEC) sensing platform based on carbon quantum dots (CQDs) modified titanium dioxide nanorods (TiO NRs), coupling with exonuclease I (Exo I) assisted in target recycling for significant signal amplification. CQDs/TiO NRs with high visible-light absorption ability and electron-hole separation efficiency is used as photoactive substrate for anchoring anti-PCB77 aptamer and its complementary DNA (cDNA). With the addition of PCB77, the specific interaction between PCB77 and its aptamer forces aptamer to separate from the electrode surface, resulting in an increase in photocurrent density.

Bovine Serum Albumin Template-Mediated Fabrication of Ruthenium Dioxide/Multiwalled Carbon Nanotubes: High-Performance Electrochemical Dopamine Biosensing in Human Serum.

The presence of abnormal dopamine (DA) levels may cause serious neurological disorders, therefore, the quantitative analysis of DA and its related research are of great significance for ensuring health. Herein, the bovine serum albumin (BSA) template method has been proposed for the preparation of catalytically high-performance ruthenium dioxide/multiwalled carbon nanotube (RuO/MWCNT) nanocomposites. The incorporation of MWCNTs has improved the active surface area and conductivity while effectively preventing the aggregation of RuO nanoparticles.

Visualization of polarity changes in endoplasmic reticulum (ER) autophagy and rheumatoid arthritis mice with near-infrared ER-targeted fluorescent probe.

The crucial cellular activities for maintaining normal cell functions heavily rely on the polarity of the endoplasmic reticulum (ER). Understanding how the polarity shifts, particularly in the context of ER autophagy (ER-phagy), holds significant promise for advancing knowledge of disorders associated with ER stress. Herein, a polarity-sensitive fluorescent probe CDI was easily synthesized from the condensation reaction of coumarin and dicyanoisophorone.

Bridging biological and food monitoring: A colorimetric and fluorescent dual-mode sensor based on N-doped carbon dots for detection of pH and histamine.

Rapid and sensitive monitoring of pH and histamine is crucial for bridging biological and food systems and identifying corresponding abnormal situations. Herein, N-doped carbon dots (CDs) are fabricated by a hydrothermal method employing dipicolinic acid and o-phenylenediamine as precursors. The CDs exhibit colorimetric and fluorescent dual-mode responses to track pH and histamine variations in living cells and food freshness, respectively.

Sensitive detections for three kinds of vitamin B in aqueous solution and on test paper by fluorescent dual-emission carbon dots.

Although a few of fluorescent probes based on carbon dots (CDs) for vitamin B (VB) determination have been emerged, none of them can realize the detection of different kinds of VB. In this paper, nitrogen, chlorine co-doped dual-emission CDs (N, Cl-CDs) with emissions at 404 nm and 595 nm have been easily synthesized. VB, VB and VB can all induce obvious fluorescence turn-off response toward the N, Cl-CDs.

A specific dual-locked fluorescence probe to visualize the dynamic changes of lipid droplets and hypochlorous acid in inflammation.

Inflammation is a key factor leading to the occurrence and development of many diseases, both lipid droplets (LDs) and hypochlorous acid (HClO/ClO) are regarded as the important biomarkers of inflammation. Therefore, it is of great significance to develop an efficient single chemical sensor that can simultaneously detect these two biomarkers. To achieve the goal, we developed a dual-locked fluorescence probe (TPA-DNP) by fusing two targets activated reporting system, its implementation was achieved by turning-on the fluorescence of TPA-DNP through LDs and HClO/ClO simultaneously.

Design of a dual-mode ratiometric fluorescent probe via MOF-on-MOF strategy for Al (III) and pH detection.

Background: The construction of ratiometric fluorescent MOF sensors with integrated self-calibration and dual-channel detection can efficiently overcome the deficiencies of single-signal sensing. In this regard, the rational design of structurally functionalized MOFs is paramount for enhancing their performance in ratiometric fluorescent sensors. Lately, the concept of MOF-on-MOF design has garnered notable interest as a potential strategy for regulating the structural parameters of MOFs by integrating two or more distinct MOF types.