Visualization procedures for proteins and peptides on flat-bed monoliths and their effects on matrix-assisted laser-desorption/ionization time-of-flight mass spectrometric detection.

The present study concerns the application of visualization methods, i.e. coomassie-brilliant-blue-R staining (CBB-R), silver-nitrate staining, and fluorescamine labeling, and subsequent MALDI-MS analysis of intact proteins and peptides on the surface of flat-bed monoliths, intended for spatial two-dimensional chromatographic separations.

Pareto-optimality study into the comparison of the separation potential of comprehensive two-dimensional liquid chromatography in the column and spatial modes.

The expected performance of spatial ("flat-bed") two-dimensional liquid chromatography ((x)LC×(x)LC) has been calculated using the Pareto-optimality strategy. This approach allowed different objectives (total peak capacity, total analysis time, and total dilution) to be considered simultaneously and to establish optimal parameters (pressure drop, particle size, bed length, and initial spot size). The performance of spatial two-dimensional chromatographic systems was compared with that of conventional on-line, real-time two-dimensional column-liquid-chromatography systems ((t)LC×(t)LC).

Hydrodynamic chromatography of macromolecules using polymer monolithic columns.

The selectivity window of size-based separations of macromolecules was tailored by tuning the macropore size of polymer monolithic columns. Monolithic materials with pore sizes ranging between 75 nm and 1.2 μm were prepared in situ in large I.

Construction and initial evaluation of an apparatus for spatial comprehensive two-dimensional liquid-phase separations.

Spatial comprehensive two-dimensional chromatography is discussed as a potentially alternative to the conventional column-based approach. In "spatial" separations each analyte ends up in a specific location, rather than being eluted at a specific time. Ultimately, higher peak-capacity-production rates (peak capacity per unit time) may be attained by spatial two- and three-dimensional separations.