Publications by authors named "Dominique Granato"
Article Synopsis
- Heat shock proteins, specifically GroEL or Hsp60 class, are vital for all organisms and have roles in cell attachment and immune modulation beyond their traditional intracellular function.
- Research focused on GroEL protein from the probiotic Lactobacillus johnsonii La1 showed it is present on the bacterial surface and can be detected in culture media.
- The study found that recombinant La1 GroEL can bind to mucins and intestinal cells in a pH-dependent manner and stimulates immune responses, suggesting its potential role in gut health by interacting with mucosal components and affecting pathogenic bacteria like H. pylori.
The aim of this work was to identify Lactobacillus johnsonii NCC533 (La1) surface molecules mediating attachment to intestinal epithelial cells and mucins. Incubation of Caco-2 intestinal epithelial cells with an L. johnsonii La1 cell wall extract led to the recognition of elongation factor Tu (EF-Tu) as a novel La1 adhesin-like factor.
View Article and Find Full Text PDFIntestinal epithelial cells (IECs) respond to lipopolysaccharide (LPS) from gram-negative bacteria in the presence of the soluble form of CD14 (sCD14), a major endotoxin receptor. Since sCD14 is also known to interact with gram-positive bacteria and their components, we looked at whether sCD14 could mediate their effects on human IECs. To this end, we examined the production of proinflammatory cytokines following exposure of the IECs to specific gram-positive bacteria or their lipoteichoic acids (LTAs) in the absence and presence of human milk as a source of sCD14.
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