Evaluation of canine adipose-derived multipotent stromal cell differentiation to ligamentoblasts on tensioned collagen type I templates in a custom bioreactor culture system.

Objective: To evaluate differentiation of canine adipose-derived multipotent stromal cells (ASCs) into ligamentoblasts on tensioned collagen type I (Col1) templates in a perfusion culture system.

Samples: Infrapatellar fat pad ASCs from healthy stifle joints of 6 female mixed-breed dogs.

Procedures: Third-passage ASCs (6 × 10 cells/template) were loaded onto suture-augmented Col1 templates under 15% static strain in perfusion bioreactors.