Publications by authors named "Dominik von La Roche"
While B cell development in the birds' primary B cell organ, the bursa Fabricius, is relatively well understood, very little is known about post bursal B cell differentiation into plasma and memory cells though these cells are essential for a protecting antibody response and so far, no specific markers for these cells were available. Since immunoglobulin class switch is one part of the B cell differentiation process, our objective was to conduct a first detailed investigation of class-switched chicken B cells. As only very few IgY and IgA expressing cells were detected in lymphoid organs of young chickens, we used CD40L and IL-10 to establish a prolonged culture system, which induces B cell proliferation, class switch to IgY and IgA and enhanced antibody secretion.
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- Flow cytometry is an important tool for monitoring immune responses in both humans and animals, including cats, but requires prompt processing of blood samples for accurate analysis.
- The SMART Tube system enables long-term storage of whole blood samples at -80°C, but had only been used for human samples until now.
- A new protocol for flow cytometry using SMART Tubes has been developed for feline blood samples, allowing analysis of various immune cells up to 2 years after collection, thereby enhancing veterinary research possibilities.
Marek's disease virus (MDV) is an oncogenic alphaherpesvirus that causes deadly lymphomas in chickens. In chickens, up to 50% of all peripheral T cells are gamma delta (γδ) T cells. Until now, their role in MDV pathogenesis and tumor formation remains poorly understood.
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- The study examines the nutritional physiology and feeding behavior of the African clawed frog (Xenopus laevis), which has been used in research for years but lacks standardized dietary practices.
- In the first trial, adult female frogs were compared on two different diets (Xenopus diet vs. fish feed) for four weeks, finding no significant impact on their body metrics or nutrient content, with body fat increasing based on body weight.
- The second trial utilized a digital tracking system to observe feeding habits and activity after meals with different diet types, revealing that frogs actively fanned food into their mouths, with feeding behavior influenced by the physical characteristics of the diets, highlighting the need for proper feeding regimens for lab welfare.
The bursa of Fabricius (BF) plays a central role in the development of B lymphocytes in birds. During embryonic development the BF primordium is colonized by myeloid and lymphoid prebursal stem cells to form the follicle buds, which ultimately develop into lymphoid follicles with a central medullary and an outer cortical region. Lympho-myeloid differentiation within the medulla is fundamental to normal B cell development.
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