Publications by authors named "Dominik Humer"
Article Synopsis
- Researchers studied the role of PKD3, a member of the Protein kinases D family, in T cell responses, particularly how it differs from the better-understood PKD2.
- Using a mouse model that overexpresses PKD3 in T cells, they found an increase in central memory T cells, though this was not fully explained by lab tests and seemed tied to developmental changes during thymic development.
- Ultimately, they concluded that PKD3 plays a subtle role in shaping the development of central memory and CD8 T cells, but it doesn't significantly affect overall immune responses in specific tests.
Background: NR2F6 has been proposed as an alternative cancer immune checkpoint in the effector T cell compartment. However, a realistic assessment of the in vivo therapeutic potential of NR2F6 requires acute depletion.
Methods: Employing primary T cells isolated from Cas9-transgenic mice for electroporation of chemically synthesized sgRNA, we established a CRISPR/Cas9-mediated acute knockout protocol of Nr2f6 in primary mouse T cells.