Publications by authors named "Dominic Goj"
Mycobacterium marinum CAR (MmCAR) is one of the most widely used CARs as the key enzyme for the synthesis of aldehydes, alcohols and further products from the respective carboxylic acids. Herein, we describe the first functionally secreted 131 kDa CAR and its isolated A-domain using Komagataella phaffii and a methanol-free constitutive expression strategy. Precipitated and lyophilized MmCAR (500 µg) was isolated from the culture supernatant and showed no decrease in activity for piperonylic acid (80% conversion), even when stored for up to 3 weeks at 4°C.
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- Enzymes are typically stereospecific to chiral substrates, yet the FsqB enzyme can oxidize both enantiomers of N-methyl-dopa, prompting a study to uncover this unusual behavior.
- Primary experiments indicated that specific tyrosine and aspartate residues in the active site of FsqB may help bind N-methyl-dopa, positioning it favorably for oxidation.
- Through various experimental and molecular simulations, researchers determined that these residues, particularly Y121, significantly influence the enzyme's ability to recognize and react with different substrates, shedding light on FsqB's unique substrate specificity.
The subfamily of sarcosine oxidase is a set of enzymes within the larger family of amine oxidases. It is ubiquitously distributed among different kingdoms of life. The member enzymes catalyze the oxidization of an N-methyl amine bond of amino acids to yield unstable imine species that undergo subsequent spontaneous non-enzymatic reactions, forming an array of different products.
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