Publications by authors named "Domenic Ebert"
Article Synopsis
- Variance in optical mesoscopic probes limits applications, especially as smaller probes show greater relative variance.
- Specific viral protein cages, like the murine polyoma virus, can assemble efficiently and accurately, minimizing statistical fluctuations due to quality control.
- An approach leveraging this assembly results in multichromophore particles that produce brighter, more consistent fluorescence than existing fluorescent nanosphere probes, validated by mass spectrometry and fluorescence microscopy.
BK polyomavirus (BKPyV) is an opportunistic pathogen that uses the b-series gangliosides GD1b and GT1b as entry receptors. Here, we characterize the impact of naturally occurring VP1 mutations on ganglioside binding, VP1 protein structure, and virus tropism. Infectious entry of single mutants E73Q and E73A and the triple mutant A72V-E73Q-E82Q (VQQ) remains sialic acid dependent, and all three variants acquire binding to a-series gangliosides, including GD1a.
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