Changes in the functional capacity of marrow stromal cells after autologous bone marrow transplantation.

Marrow stromal cells were evaluated several months after autologous BMT for their capacity to support both normal hemopoiesis and secrete the main growth factors involved in its control, G-CSF, GM-CSF, IL-3 and SCF. Stromal layers (SL) were obtained by long-term marrow cultures (LTMC) established from 15 patients (9 with hematologic malignancies and 6 with solid tumors) 3 months after autologous BMT and were compared to pre-graft patients. After irradiation, both post-graft and pre-graft SL were recharged with the same inoculum of normal marrow cells.

Influence of d-limonene on the transdermal penetration of felodipine.

Felodipine is a calcium antagonist, one of the dihydropyridines, with potential application in transdermal therapeutic systems (TTS). Earlier studies reported that the high lag time of this drug limited its potential development in a TTS. The present study analyzes the effect of d-limonene at concentrations of 0.

Morgagni's hernia resolved by laparoscopic surgery.

Morgagni's hernias represent between 2 and 4% of the whole of nontraumatic diaphragmatic hernias in the adult, and the treatment (even with minimal symptoms) is always with surgery. We present the case of a 50-year-old woman with an oppressive, postprandial discomfort in the right side of her thorax and with increased pain when supine. The x-ray examination indicated a large portion of transverse colon inside the thoracic cavity.

Structure of complex cell wall polysaccharides isolated from Trichoderma and Hypocrea species.

The structure of fungal polysaccharides isolated from the cell wall of Trichoderma reesei, T. koningii, and Hypocrea psychrophila, have been investigated by means of chemical analyses and 1D and 2D NMR spectroscopy. The polysaccharides have an irregular structure, idealized as follows: [formula: see text] The proportions of the different side chains vary from a species to another, being n above some three times larger in H.

Line-Mixing Effects in Q Branches of CO2.

A theoretical model based on the energy corrected sudden (ECS) approximation is used to account for line-mixing effects in infrared Q branches of parallel bands of CO2. Measurements have been made using a tunable difference frequency spectrometer system in a Pi-Pi transition: spectra of the 11(1)1II <-- 01(1)0I Q branch near 3580.3 cm-1 have been recorded at room temperature for CO2-He and CO2-N2 mixtures in the 0.

Influence of formulation on the in vitro transdermal penetration of flutrimazole.

Flutrimazole (1-[(2-fluorophenyl)(4-fluorophenyl)phenylmethyl]-1 H-imidazole, CAS 119006-77-8, UR-4056) is a new wide spectrum local imidazolic antifungal agent that has already been formulated as a dermal cream (FDC). A comparative study was carried out of the release of flutrimazole from two emulsions in which the drug has been incorporated differently: one dissolved in the oily phase (E24) and the other dispersed in the aqueous formulation phase (E25). Based on the E25 formulation, two more dermal creams were prepared, E27 with benzyl alcohol and E28 with diazolidinyl urea as preservative agents.

Structure-absorption relationships of a series of 6-fluoroquinolones.

The physicochemical constants and some structural parameters (topological, steric, and electronic) of eight third-generation monofluorate quinolones (six uncommercialized and two used clinically [ciprofloxacin and enrofloxacin]) were determined: pKa, intrinsic solubility (S0), chromatographic capacity factor, partition coefficient (P), valency molecular connectivity, molecular volume, molecular surface area, dipolar moment, and charges associated with each atom of the molecule. The apparent intestinal absorption rate constants (K(abs)) in rat (in vivo perfusion) and the MICs at which 90% of the isolates are inhibited (MIC90s) against 100 Escherichia coli strains were also determined. We sought to establish simple nonlinear and multiple linear correlations between K(abs), on the one hand, and lipophilic parameters and other physicochemical and structural parameters estimated.

Absolute Line Intensities in the 2nu3 Band of 16O12C32S.

The strengths of 100 lines in the 2nu3 band of 16O12C32S have been measured at high resolution in the spectral range 4069-4118 cm-1, using a tunable difference-frequency laser spectrometer. These intensities were obtained by fitting Voigt profiles to the measured shapes of the lines. The vibrational transition moment [(2.

Structural elucidation of acidic fungal polysaccharides isolated from the cell-wall of genera Cylindrocladium and Calonectria.

The structure of acidic fungal polysaccharides, isolated from the cell wall of Cylindrocladium and Calonectria species, has been investigated by chemical analysis, methylation and reductive cleavage analyses, and 1D and 2D 1H and 13C NMR spectroscopy. The polysaccharides have an idealized repeating unit: [formula: see text] linked to a small mannan core (< 5%), with n = 3 for Cylindrocladium penicilloides and C. quinqueseptatum and n = 5 for Calonectria theae, C.

Infrared Diode-Laser Molecular-Beam Spectrum of the nu2 Band of Chlorine Nitrate at 1293 cm-1.

The nu2 band of chlorine nitrate (ClONO2 ) near 1293 cm-1 has been measured in a molecular beam with a diode-laser spectrometer. The low rotational temperature of the molecular beam, approximately 23 K, simplifies the spectrum allowing essentially complete assignment of the 35 Cl and 37 Cl lines. An a /b hybrid band is observed with the a -type transition moment being approximately a factor of 2 larger than the b -type transition moment.

Diagnostic agreement between clinicians and the Diagnostic Interview for Children and Adolescents--DICA-R--in an outpatient sample.

The paper examines the diagnostic agreement between clinicians and the Diagnostic Interview for Children and Adolescents. One hundred and thirty-seven outpatients-children and adolescents, and their parents-were diagnosed independently following DSM-III-R criteria by clinicians and by the DICA-R. The diagnostic concordance between clinicians and DICA-R ranged from low to moderate in the majority of the categories.

A comparative study of the transdermal penetration of a series of nonsteroidal antiinflammatory drugs.

The transdermal absorption of a series of nonsteroidal antiinflammatory drugs (NSAIDs): indomethacin, ketoprofen, diclofenac, piroxicam, tenoxicam, ketorolac, and aceclofenac) was studied in vitro with human skin. The purpose of the study was to determine the permeation parameters (permeability rate constant, Kp; lag time, TL, and flux, J) as measures of the intrinsic transdermal permeabilities of these drugs to predict their potential for formulation in a transdermal therapeutic system (TTS). A linear correlation was established between the intrinsic log Kp values and the intrinsic partition coefficients (r = 0.

The mechanisms involved in the impairment of hematopoiesis after autologous bone marrow transplantation.

Hematopoiesis after autologous bone marrow transplantation (BMT) is characterized by a prolonged and severe deficiency of marrow progenitors for several years, especially of erythroid and megakaryocyte progenitors, while the peripheral blood cells and marrow cellularity have reached relatively normal values within a few weeks. These anomalies are comparable to those reported for allogeneic BMT, despite the absence of any allo-immune reaction or post-graft immunosuppressive therapy. Post-graft hematopoietic impairment is the consequence of quantitative and qualitative changes involving both stem cell and stromal compartments which are expressed by an impaired capacity of stem cell self-renewal and commitment towards erythroid and megakaryocytic lineages.

Manifestation and epidemiology of contagious bovine pleuropneumonia in Africa.

Contagious bovine pleuropneumonia (CBPP) is one of the major threats to cattle health and production in Africa. This article reviews the clinical manifestations, lesions and epidemiology of the disease. The clinical manifestations and lesions are typical and are no different in Africa from those seen in other countries.

[Equilibrium and auditory disorders in patients affected by HIV-1].

It is thought that about 10% of the patients with advanced human immunodeficiency virus (HIV) infection have otoneurological symptoms, although up to 41% of these patients have general otorhinolaryngological symptoms. The otological changes may be divided into those of balance and those of hearing, to improve classification, although these symptoms are often to be found together in the same patient. We studied 30 seropositive patients with stage II and stage III disease, carrying out studies of oculomotricity, electronystagmography and audiometry (both conventional and high frequency).

An antigenic water-soluble glucogalactomannan extracted from cell walls of Paecilomyces fumosoroseus and Paecilomyces farinosus.

The water-soluble fraction (F1S) obtained after solubilizing in alkali the cell walls of four strains of Paecilomyces fumosoroseus and two of Paecilomyces farinosus amounted to 8.3-14.5% of the dry cell wall material.

Obesity, diet and restrained eating in a Mediterranean population.

Objective: To evaluate links between obesity, gender and restrained eating in a representative sample of adolescents in Catalonia, Spain.

Design: Several surveys were conducted in which measurements were taken of a sample of adolescents living in the city of Barcelona.

Subjects: 610 subjects (57% girls and 43% boys) with ages ranging between 15-17, from 22 schools in Barcelona.

Granulocyte-macrophage colony-stimulating factor accelerates hematopoietic recovery after autologous bone marrow or peripheral blood progenitor cell transplantation and high-dose chemotherapy for lymphoma.

The use of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) as an adjunct to autologous bone marrow transplantation (ABMT) or peripheral blood progenitor cell (PBPC) transplantation was evaluated in 59 lymphoma patients. Patients were divided into three groups. In group I (n = 21) patients received rhGM-CSF (5 micrograms/kg daily) at the time of PBPC collection and during the recovery phase post-infusion.

Palmar dislocation of the metacarpophalangeal joint of the thumb: a case report.

A case of palmar dislocation of the metacarpophalangeal joint of the thumb is presented. The interposition of the dorsal capsule and the extensor tendons made open reduction necessary.

[Right ventricular myxoma. A rare case of pulmonary stenosis].

We discuss a case of a fourteen year old girl in whom, clinical signs of right ventricular outflow obstruction were discovered following a syncopal attack. A right ventricular tumor was observed by echocardiography. Histology confirmed the diagnosis of myxoma.

Distal tibial triplane fractures: long-term follow-up.

The cases of 35 patients in whom a triplane fracture had been treated at the Ramón y Cajal Hospital were reviewed. The diagnosis of biplane and triplane fracture can now be made by standard radiograph. However, plain radiographs alone did not accurately demonstrate the configuration of the fracture, and computed tomography must be performed.

Long-term cultures to evaluate engraftment potential of CD34+ cells from peripheral blood after mobilization by chemotherapy with and without GM-CSF.

In this study we used a long-term culture system to evaluate engraftment potential of human peripheral blood (PB) cells mobilized by chemotherapy (CT) associated or not with granulocyte-macrophage colony-stimulating factor (GM-CSF). In six patients who underwent blood cell transplantation, PB CD34+ cells were cultured after mobilization and were compared to CD34+ cells in steady state from PB and bone marrow (BM). Qualitative differences were shown between PBC samples obtained after CT with and without GM-CSF.

Electrocardiographic diagnosis of the responsible coronary artery in acute inferior myocardial infarction through right chest leads V3R-V8R. A prospective study.

In order to identify the electrocardiographic changes that occur in right-chest leads V3R-V8R for the most significant diagnosis of the responsible coronary artery of acute myocardial infarction, the authors performed a prospective study on 66 patients in whom coronary arteriography was done between the first and twelfth weeks after suffering the infarction. Electrocardiograms were done within the first six hours after the onset of symptoms. Lesions of the right coronary artery were found in 46 patients--27 at a proximal level and 19 at a distal one--and in 20 patients the circumflex coronary artery was injured.