[Does elitism of school influence the smoking-related health behaviour among grammar school students?].

The aim of the study was connected with smoking health behaviour estimation among public (SZP) and nonpublic (SZN) grammar school students. The analysis of 156 anonymous questionnaires was made. Questionnaires contained questions of parents' education, material situation of family, physical education, social relations with family and peers and positive or negative perception of smoking.

Muscle-invasive urothelial cell carcinoma of the human bladder: multidirectional differentiation and ability to metastasize.

Few published studies have addressed the correlation between multidirectional differentiation in muscle-invasive bladder cancer and its ability to metastasize. We demonstrated that histologic differentiation within a single tumor affects lymph node metastasis. We examined cystectomy specimens from 93 bladder tumors and 1085 lymph nodes.

Morphologic changes in the canine urinary tract after ureteral reconstruction with the tunica albuginea (experimental study).

Objectives: The problem of ureteral reconstruction has not been fully solved, despite dramatic developments in surgical methods. Many attempts to repair a ureteral damage with autologous or homologous organ grafts, or with organic substitutes have not really been satisfactory. Also, attempts to use alloplastic and heteroplastic prostheses made of synthetic materials have been equally disappointing.

Ultraviolet radiation (UV) induces reorganization of actin cytoskeleton in CHOAA8 cells.

Effect of UV radiation on actin cytoskeleton was studied in CHOAA8 cells by fluorescence and electron microscopy. UV irradiated cells showed impaired adherence, disruption of the actin filaments and stronger F-actin labeling in the center of the cell. Attached cells, especially enlarged ones showed rather weak labeling of stress fibers and bundles of F-actin in the cytoplasm, but some cells with intensive labeling of these structures were also observed.

Usefulness of histologic homogeneity estimation of muscle-invasive urinary bladder cancer in an individual prognosis: a mapping study.

Objectives: To examine the histologic homogeneity of muscle-invasive urothelial cell carcinoma of the bladder, with conventional and nonconventional (eg, squamous, glandular, or variants) differentiation, to assess its influence on prognosis.

Methods: With organ mapping, we investigated 38 cystectomy specimens. Each entire bladder was cut into 88 slices according to an identical topographic scheme.

Effect of doxorubicin on actin reorganization in Chinese hamster ovary cells.

Exposure of Chinese hamster ovary (CHO AA8) cells to doxorubicin in doses of 75 microM resulted in reorganization of F-actin filaments and characteristic feature of apoptosis. Even increase in size of CHOAA8 cells was observed. Attached cells become more flattered and elongated than control ones.

Electron-microscopic observation of adherence of Acinetobacter baumannii to red blood cells.

It is now recognized that Acinetobacter spp. play a significant role in the colonization and infection of patients admitted to hospitals. The virulence factors of A.

A comparison of three staining methods in estimation of structures on the cell wall surface of Acinetobacter junii by using electron microscope.

Two strains of Acinetobacter junii isolated from groin and nasal cavity were studied for the presence of structures on the cell wall surface. Three staining methods were used. In the first thin section were contrasted with uranyl acetate and lead citrate.

[Pneumocystis pneumonia in light of personal observations].

During a period of 10 months, 87 children with Pneumocystis carinii pneumonia accompanied transient cellular immunity disorders and with normal humoral immunity were observed. It is suggested that change the invasiveness of the parasite has changed. The necessity of taking into consideration a pneumocystis etiology in diagnosis of respiratory tract diseases among children is pointed out.

Analysis of the low molecular weight nuclear polypeptide by isoelectrofocusing of nuclear proteins first separated by gel electrophoresis in SDS.

Some of the nuclear proteins, especially those tightly bound with nucleic acids, show a tendency to aggregate and some of them are also hydrophobic. Thus, SDS-containing buffers are useful for their analysis. The method of isoelectrofocusing of proteins separated by polyacrylamide gel electrophoresis in the presence of SDS was used for the analysis of the nuclear proteins.

Ultrastructural evaluation of changes in the glomeruli occurring in association with renal carcinoma.

The studies aimed at the determination of ultrastructural changes occurring in the glomeruli in association with renal carcinoma. The material was taken from 7 patients with renal carcinoma. Electron-microscopic investigation showed changes in all 7 cases.

Relation between ultrastructural presence of CEA using peroxidase-antiperoxidase (PAP) method and tissue and serum levels of CEA in patients with breast cancer.

Ultrastructural studies were performed to detect the presence of carcinoembryonic antigen (CEA) in breast cancer by peroxidase-antiperoxidase (PAP) method. The evidenced presence of CEA was compared with the serum and tissue concentrations. A correlation between the presence of CEA at the ultrastructural level and tissue concentration was observed but not with serum levels.

Characterization of herpes simplex virus type 1 DNA during latent infection in mice.

Micrococcal nuclease digestion technique in combination with spot-blotting hybridization have been used for the characterization of latent Herpes simplex virus type 1 (HSV-1) DNA in mouse brainstems. Two kinds of samples were used i.e.

[Trial application of transfection tests in feasibility studies for transforming DNA in white blood cells of patients with chronic lymphatic leukemia].

Transfection technique with the use of high molecular DNA was applied for the investigations of peripheral blood white cells DNA transforming properties in patients with chronic lymphatic leukemia B. Using transfection tests in liquid medium (focus assay) and colony forming in soft agar, the differences in L(tk-) cells transformation were noted following an addition of neoplastic DNA. Medium collected over transformants has shown mitogen properties in micro-mitogen tests on cells BALB/c 3T3.

[Ultrastructural evaluation of carcinoembryonic antigen (CEA) using an immunocytochemical method with the peroxidase-antiperoxidase (PAP) complex technic in patients with laryngeal cancer].

Peroxidase-antiperoxidase complex method (PAP) was used to reveal the CEA antigen in the larynx carcinoma cells by means of electron microscope. Studies were carried out on the material taken from 8 patients with the larynx carcinoma. In all cases positive immunoperoxidase reaction has been observed.

[Ultrastructural study of carcinoembryonic antigen (CEA) using the peroxidase-antiperoxidase (PAP) complex technic in patients with breast cancer].

In the studied material taken from 70 patients with the mammary carcinoma was tried to discover the presence of the CEA antigen in the cells on the ultrastructural level. In studies was used peroxidase-antiperoxidase method (PAP). The product of the immunocytochemical reaction was found on the surface of cell membrane.

[Ultrastructural evaluation of mast cells in food allergy].

In this paper we described the fine structure changes observed in mast cells from patients with food allergy. We studied material from 7 patients with allergy for the eggs and flour. Biopsies were obtained from the gastric mucosa, duodenum and rectum after the allergic provocation.

[Ductal carcinoma of the breast. Electron microscopic study].

Electron microscopic study of the breast tumors were carried out on 67 patients. There were 7 intraductal carcinomas and 60 infiltrating ductal carcinomas of the breast. Observations were made on the morphological characteristics of neoplastic cells, myoepithelial cells and basal lamina.