Sustainable livestock management under anthropogenic pressure: Bridging traditional herding and contemporary conservation in Eurasia's oldest protected area.

In some regions of the world, long traditions of herding practices have coevolved with the natural ecosystems, sustaining livelihoods and biodiversity. However, in emerging economies, the populations that have long relied on livestock as their main income are now driving a dramatic increase in livestock numbers on the landscape. This study investigates the impacts of livestock (cattle and horses) and human activities on wild ungulates, Siberian roe deer (Capreolus pygargus), wapiti (Cervus canadensis), and wild boar (Sus scrofa), in Bogd Khan Mountain, Mongolia.

Development of 3D printed mesofluidic devices to elute and concentrate DNA.

To understand structural variation for personal genomics, an extensive ensemble of large DNA molecules will be required to span large structural variations. Nanocoding, a whole-genome analysis platform, can analyze large DNA molecules for the construction of physical restriction maps of entire genomes. However, handling of large DNA is difficult and a system is needed to concentrate large DNA molecules, while keeping the molecules intact.

Selective analysis of secondary amino acids in gelatin using pulsed electrochemical detection.

A method was developed for selective analysis of the secondary amino acids proline and 4-hydroxyproline from gelatin hydrolysates using anion-exchange high-performance liquid chromatography followed by integrated pulsed amperometric detection (HPLC-IPAD). An extraction scheme was implemented prior to HPLC-IPAD analysis to isolate the secondary amino acids by the removal of primary amino acids through derivatization with o-phthalaldehyde followed by solid-phase extraction with C18 packed columns. The use of the IPAD technique eliminated the need for a second derivatization step to detect secondary amino acids.

Idiopathic retroperitoneal haemorrhage.

Idiopathic retroperitoneal haemorrhage is a rare condition, affecting apparently histologically normal retroperitoneal blood vessels. It is important to search for the source of bleeding, as failure to identify the cause is associated with poor prognosis.

Analysis of fatty acids as their anthrylmethyl esters by high-performance liquid chromatography with fluorescence detection.

A reversed-phase high-performance liquid chromatographic method for the analysis of free fatty acids in plasma has been developed which allows the resolution of the major plasma fatty acids in man. A mixture of thirteen fatty acid anthrylmethyl esters is resolved on a Spherisorb 3-micron C8 column at a flow-rate of 1 ml/min by gradient elution. The solvent system consists of acetonitrile-water (93:7) for 12 min, followed by 5 min at acetonitrile-water (86:14) and 23 min at 100% acetonitrile.