High-throughput antibody screening with high-quality factor nanophotonics and bioprinting.

Empirical investigation of the quintillion-scale, functionally diverse antibody repertoires that can be generated synthetically or naturally is critical for identifying potential biotherapeutic leads, yet remains burdensome. We present high-throughput nanophotonics- and bioprinter-enabled screening (HT-NaBS), a multiplexed assay for large-scale, sample-efficient, and rapid characterization of antibody libraries. Our platform is built upon independently addressable pixelated nanoantennas exhibiting wavelength-scale mode volumes, high-quality factors (high-Q) exceeding 5000, and pattern densities exceeding one million sensors per square centimeter.

Very-large-scale-integrated high quality factor nanoantenna pixels.

Metasurfaces precisely control the amplitude, polarization and phase of light, with applications spanning imaging, sensing, modulation and computing. Three crucial performance metrics of metasurfaces and their constituent resonators are the quality factor (Q factor), mode volume (V) and ability to control far-field radiation. Often, resonators face a trade-off between these parameters: a reduction in V leads to an equivalent reduction in Q, albeit with more control over radiation.

Rapid genetic screening with high quality factor metasurfaces.

Genetic analysis methods are foundational to advancing personalized medicine, accelerating disease diagnostics, and monitoring the health of organisms and ecosystems. Current nucleic acid technologies such as polymerase chain reaction (PCR) and next-generation sequencing (NGS) rely on sample amplification and can suffer from inhibition. Here, we introduce a label-free genetic screening platform based on high quality (high-Q) factor silicon nanoantennas functionalized with nucleic acid fragments.

[Primary structure of RNA 3 of barley stripe mosaic virus and its variability].

The complete nucleotide sequence was determined for three variants of the third genomic component of BSMV strain Argentina mild. The common variant, RNA 3 (2797 nucleotide), contains two open reading frames (ORFs) coding for two proteins with Mr of 74,229 (putative BSMV RNA polymerase) and Mr of 16,994. The second ORF is expressed from a subgenomic RNA.

[Translation of the genomic and subgenomic RNAs of cucumber virus 3 in a cell free system from wheat embryos and in Xenopus laevis oocytes].

The purified preparation of cucumber virus 3 (CV3) (one of the member of tobamovirus group) contains, besides full-length (2.0 X 10(6)) genomic RNA, a short (0.24 X 10(6)) subgenomic RNA coding for coat protein in Xenopus laevis oocytes as well as in the cell-free protein synthesizing system from wheat embryos.