[Modulation of voltage-gated sodium channels by recombinant interferon-alpha2b in the human neuroblastoma cell line IMR-32].

Clonal human neuroblastoma cells imr-32 are a suitable model system for studies of neuronal excitability modulation. The ability interferon-alpha 2b "laferon" to modulate the mechanisms of electrical activity was studied in whole-cell patch-clamped undifferentiated human neuroblastoma cells IMR-32. It was shown that 1 h incubation of IMR-32 cells at 37 degrees C in medium with laferon (600 U/ml) exerted changes in voltage-dependent properties of Na(+)-channels.

[Surface charge of the cytoplasmic side of the cell membrane of neuroblastoma in murine line C1300].

Inside-out vesicles were obtained from the cell membranes of murine neuroblastoma. The surface charge of vesicles was studied by the microelectrophoresis method. At neutral pH they had the electrophoretic mobility 2.

[Relation between the surface potential of mouse neuroblastoma clone C1300 cells and the phase of the cell cycle].

The surface charge of neuroblastoma cells in different phases of the cell cycle was studied by the microelectrophoresis method. The surface charge increased by 50% on the average after addition of colchicine to the culture medium, by 20-30% after addition of dibutyryl-cAMP or removal of the serum from the medium and decreased by 30% after addition of DMSO. These changes correlated well with variations of the protein content per cell.

[Surface charge of mouse neuroblastoma cells and changes during growth and morphologic differentiation of the cell population].

Microelectrophoresis method was used to study the surface charge of murine neuroblastoma cells (clone C1300-N18TG2). It was shown that the surface charge of these cells was determined mainly by anionic groups of the membrane which were distributed with density 0.2 e/nm3 in the layer covering its outer surface.

[Study of the surface properties of spinal ganglion neurons in the rat by the microelectrophoresis technic].

Surface charges of isolated neurons of rat dorsal root ganglion were studied by the microelectrophoresis. It was shown that the surface potential of these neurons is produced by anion groups which form complexes with calcium ions and a binding constant ranging from 10 to 50 1/mole; they can be titrated by hydrogen ions according to pK = 3.8.

[Quantitative description of a slowly inactivating barium current through the somatic membrane of mollusk neurons].

The slow-inactivating barium current was recorded under voltage clamp conditions from identified neurons of the snail Helix pomatia. Experimental data were analyzed using the Hodgkin-Huxley equations. The dependence of the steady-state value of the inactivation variable on membrane potential was obtained.

[Effect of calcium ions on potential-dependent potassium channels in the membrane of the soma of giant mollusc neurons].

The delayed and fast outward currents in the somatic membrane of mollusc giant neurons were studied under voltage-clamp conditions in normal (10 mM) and low-Ca (I mM) saline. At pH 7.2 a tenfold decrease in calcium concentration in the peak conductance vs membrane potential curves for delayed and fast outward currents were shifted along the voltage axis by 10 mV.