Geographic Variation in Racial Disparities in Age-Adjusted Mortality Rates in Mississippi.

Background: Racial disparities in mortality rates have been well-documented in the last century. Intersectionality theory has helped to identify the root causes of these health disparities. Few studies have examined disparities using the latest data for the state of Mississippi.

Deficit irrigation differentially modulates rhizosphere microbial community and metabolites of two potato genotypes differing in drought tolerance.

Beneficial interactions between plant root exudates and the rhizosphere microbial community can alleviate the adverse effects of environmental stress on crop yields, but these interactions remain poorly understood in potato growing in drying soil. We investigated the responses of rhizosphere soil microorganisms and metabolites, and biochemical and physiological responses of two potato genotypes with contrasting drought tolerance (drought tolerant 'C93' and drought sensitive 'Favorita'), to two different irrigation treatments imposing contrasting soil water availability in the field. Deficit irrigation altered rhizosphere soil bacterial communities and metabolites of C93 more than Favorita.

European soybean to benefit people and the environment.

Europe imports large amounts of soybean that are predominantly used for livestock feed, mainly sourced from Brazil, USA and Argentina. In addition, the demand for GM-free soybean for human consumption is project to increase. Soybean has higher protein quality and digestibility than other legumes, along with high concentrations of isoflavones, phytosterols and minerals that enhance the nutritional value as a human food ingredient.

ABA is required for differential cell wall acidification associated with root hydrotropic bending in tomato.

Hydrotropism is an important adaptation of plant roots to the uneven distribution of water, with current research mainly focused on Arabidopsis thaliana. To examine hydrotropism in tomato (Solanum lycopersicum) primary roots, we used RNA sequencing to determine gene expression of root tips (apical 5 mm) on dry and wet sides of hydrostimulated roots grown on agar plates. Hydrostimulation enhances cell division and expansion on the dry side compared with the wet side of the root tip.

When push comes to shove - RNA polymerase and DNA-bound protein roadblocks.

In recent years, transcriptional roadblocking has emerged as a crucial regulatory mechanism in gene expression, whereby other DNA-bound obstacles can block the progression of transcribing RNA polymerase (RNAP), leading to RNAP pausing and ultimately dissociation from the DNA template. In this review, we discuss the mechanisms by which transcriptional roadblocks can impede RNAP progression, as well as how RNAP can overcome these obstacles to continue transcription. We examine different DNA-binding proteins involved in transcriptional roadblocking and their biophysical properties that determine their effectiveness in blocking RNAP progression.

Cultivar-dependent differences in tuber growth cause increased soil resistance in potato fields.

Since soil compaction of potato fields delays shoot emergence and decreases total yield, the causes and effects of this compaction need to be better understood. In a controlled environment trial with young (before tuber initiation) plants, roots of cv. Inca Bella (a group cultivar) were more sensitive to increased soil resistance (3.

Exogenous monoterpenes mitigate H2O2-induced lipid damage but do not attenuate photosynthetic decline during water deficit in tomato.

Although monoterpenes are suggested to mediate oxidative status, their role in abiotic stress responses is currently unclear. Here, a foliar spray of monoterpenes increased antioxidant capacity and decreased oxidative stress of Solanum lycopersicum under water deficit stress. The foliar content of monoterpenes increased with spray concentration indicating foliar uptake of exogenous monoterpenes.

Similar soil drying-induced stomatal closure in soybean genotypes varying in abscisic acid accumulation and stomatal sensitivity to abscisic acid.

Different soybean cultivars (Williams 82 , Union , Jindou 21 , Long Huang 1 , Long Huang 2 ) were exposed to drying soil, to investigate whether endogenous abscisic acid (ABA) concentrations and leaf water relations regulated stomatal behaviour. We measured ABA concentrations in xylem and tissue of the first and second trifoliate leaves respectively; stomatal conductance (gs ) and leaf water potential (Ψleaf ) in both leaves; and water content in soil. Cultivar variation in leaf area and g s caused different rates of soil drying, but g s and Ψ leaf declined similarly with soil drying in all cultivars.

Prolonged low temperature exposure de-sensitises ABA-induced stomatal closure in soybean, involving an ethylene-dependent process.

Chilling can decrease stomatal sensitivity to abscisic acid (ABA) in some legumes, although hormonal mechanisms involved are unclear. After evaluating leaf gas exchange of 16 European soybean genotypes at 14°C, 6 genotypes representing the range of response were selected. Further experiments combined low (L, 14°C) and high (H, 24°C) temperature exposure from sowing until the unifoliate leaf was visible and L or H temperature until full leaf expansion, to impose four temperature treatments: LL, LH, HL, and HH.

Identifying physiological and genetic determinants of faba bean transpiration response to evaporative demand.

Background And Aims: Limiting maximum transpiration rate (TR) under high vapour pressure deficit (VPD) works as a water conservation strategy. While some breeding programmes have incorporated this trait into some crops to boost yields in water-limited environments, its underlying physiological mechanisms and genetic regulation remain unknown for faba bean (Vicia faba). Thus, we aimed to identify genetic variation in the TR response to VPD in a population of faba bean recombinant inbred lines (RILs) derived from two parental lines with contrasting water use (Mélodie/2 and ILB 938/2).

Hydraulic flux-responsive hormone redistribution determines root branching.

Plant roots exhibit plasticity in their branching patterns to forage efficiently for heterogeneously distributed resources, such as soil water. The xerobranching response represses lateral root formation when roots lose contact with water. Here, we show that xerobranching is regulated by radial movement of the phloem-derived hormone abscisic acid, which disrupts intercellular communication between inner and outer cell layers through plasmodesmata.

The Effects of High CO and Strigolactones on Shoot Branching and Aphid-Plant Compatibility Control in Pea.

Elevated atmospheric CO concentrations (eCO) regulate plant architecture and susceptibility to insects. We explored the mechanisms underpinning these responses in wild type (WT) peas and mutants defective in either strigolactone (SL) synthesis or signaling. All genotypes had increased shoot height and branching, dry weights and carbohydrate levels under eCO, demonstrating that SLs are not required for shoot acclimation to eCO.

Altered properties and structures of root exudate polysaccharides in a root hairless mutant of barley.

Root exudates and rhizosheaths of attached soil are important features of growing roots. To elucidate factors involved in rhizosheath formation, wild-type (WT) barley (Hordeum vulgare L. cv.

Ethylene inhibits rice root elongation in compacted soil via ABA- and auxin-mediated mechanisms.

Soil compaction represents a major agronomic challenge, inhibiting root elongation and impacting crop yields. Roots use ethylene to sense soil compaction as the restricted air space causes this gaseous signal to accumulate around root tips. Ethylene inhibits root elongation and promotes radial expansion in compacted soil, but its mechanistic basis remains unclear.

Rhizosheath: An adaptive root trait to improve plant tolerance to phosphorus and water deficits?

Drought and nutrient limitations adversely affect crop yields, with below-ground traits enhancing crop production in these resource-poor environments. This review explores the interacting biological, chemical and physical factors that determine rhizosheath (soil adhering to the root system) development, and its influence on plant water uptake and phosphorus acquisition in dry soils. Identification of quantitative trait loci for rhizosheath development indicate it is genetically determined, but the microbial community also directly (polysaccharide exudation) and indirectly (altered root hair development) affect its extent.

Bi-directional, long-distance hormonal signalling between roots and shoots of soil water availability.

While the importance of plant water relations in determining crop response to soil water availability is difficult to over-emphasise, under many circumstances, plants maintain their leaf water status as the soil dries yet shoot gas exchange and growth is restricted. Such observations lead to development of a paradigm that root-to-shoot signals regulate shoot physiology, and a conceptual framework to test the importance of different signals such as plant hormones in these physiological processes. Nevertheless, shoot-to-root (hormonal) signalling also plays an important role in regulating root growth and function and may dominate when larger quantities of a hormone are produced in the shoots than the roots.

Plant responses to heterogeneous salinity: agronomic relevance and research priorities.

Background: Soil salinity, in both natural and managed environments, is highly heterogeneous, and understanding how plants respond to this spatiotemporal heterogeneity is increasingly important for sustainable agriculture in the era of global climate change. While the vast majority of research on crop response to salinity utilizes homogeneous saline conditions, a much smaller, but important, effort has been made in the past decade to understand plant molecular and physiological responses to heterogeneous salinity mainly by using split-root studies. These studies have begun to unravel how plants compensate for water/nutrient deprivation and limit salt stress by optimizing root-foraging in the most favourable parts of the soil.

Ultraviolet radiation causes leaf warming due to partial stomatal closure.

Variation in solar ultraviolet radiation induces a wide-range of plant responses from the cellular to whole-plant scale. We demonstrate here for the first time that partial stomatal closure caused by ultraviolet radiation exposure results in significant increases in leaf temperature. Significant leaf warming in response to ultraviolet radiation was consistent in tomato (Solanum lycopersicum L.

Land Management Legacy Affects Abundance and Function of the Gene in Wheat Root Associated Pseudomonads.

Land management practices can vastly influence belowground plant traits due to chemical, physical, and biological alteration of soil properties. Beneficial spp. are agriculturally relevant bacteria with a plethora of plant growth promoting (PGP) qualities, including the potential to alter plant physiology by modulating plant produced ethylene the action of the bacterial enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase ().

Analysis of Infection Time Courses Shows CII Levels Determine the Frequency of Lysogeny in Phage 186.

Engineered phage with properties optimised for the treatment of bacterial infections hold great promise, but require careful characterisation by a number of approaches. Phage-bacteria infection time courses, where populations of bacteriophage and bacteria are mixed and followed over many infection cycles, can be used to deduce properties of phage infection at the individual cell level. Here, we apply this approach to analysis of infection of by the temperate bacteriophage 186 and explore which properties of the infection process can be reliably inferred.

Abscisic Acid Mediates Drought-Enhanced Rhizosheath Formation in Tomato.

The rhizosheath, commonly defined as soil adhering to the root surface, may confer drought tolerance in various crop species by enhancing access to water and nutrients under drying stress conditions. Since the role of phytohormones in establishing this trait remains largely unexplored, we investigated the role of ABA in rhizosheath formation of wild-type (WT) and ABA-deficient () tomatoes. Both genotypes had similar rhizosheath weight, root length, and root ABA concentration in well-watered soil.

The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in .

We describe a new set of tools for inserting DNA into the bacterial chromosome. The system uses site-specific recombination reactions carried out by bacteriophage integrases to integrate plasmids at up to eight phage attachment sites in . MG1655.

ABA regulation of root growth during soil drying and recovery can involve auxin response.

Abscisic acid (ABA) plays an important role in plant adaptation to water deficits, but its role in regulating root growth (primary root elongation and lateral root number) during different drought-phases remains unclear. Here, we exposed wild-type (WT) and ABA-deficient (not) tomato plants to three continuous drought-phases (moderate drying: day 0-21; severe drying: day 22-47 and re-watering: day 48-51). It was found that WT increased primary root growth during moderate drying; maintained more lateral roots, and greater primary root and total root length under severe drying; and produced more roots after re-watering.

Overproduction of ABA in rootstocks alleviates salinity stress in tomato shoots.

To determine whether root-supplied ABA alleviates saline stress, tomato (Solanum lycopersicum L. cv. Sugar Drop) was grafted onto two independent lines (NCED OE) overexpressing the SlNCED1 gene (9-cis-epoxycarotenoid dioxygenase) and wild type rootstocks.