[Effects of low intensity laser (632.8 nm) and ultraviolet radiation on spectral properties and functional activity of human ceruloplasmin].

Photoinduced changes of human ceruloplasmin (hCp) under laser and ultraviolet (UV) radiation have been studied. A complex character of hCp spectral modifications points to a different degree of the protein molecule folding. After the influence of UV radiation (240-390 nm) in a dose range 906-4530 J/m2 the decrease of oxidase hCp activity was registered.

[Diagnostic possibilities of an immunoenzyme test-system for determining the spectrum of antibodies to structural and nonstructural hepatitis C virus antigens].

A new enzyme immunoassay EIA-HCV-Spectr test system constructed on the base of recombinant proteins and synthetic peptides allows separate detection of antibodies to E1/E2, core, HS3, NS4, and NS5 antigens of hepatitis C virus (HCV). The system is highly specific and more sensitive than the test systems used in screening studies, which allows its use as a final test for antiHCV antibodies. Antibodies to various HCV antigens were analyzed using this test system in patients with acute and chronic hepatitis C and asymptomatic donors with antiHCV.

[Laboratory diagnosis of intoxication in chronic dermatosis].

The content of medium molecular weight (MMW) substances in the blood and urine was studied in patients with chronic dermatoses (psoriasis and atopic dermatitis). Intoxication was detected in all these patients, which was confirmed by clinical symptoms and a high level of plasma MMW substances, indicating pronounced catabolic processes in this condition. The distribution of MMW substances in biological media and the absence of positive changes after treatment in patients with atopic dermatitis in comparison with psoriasis patients may be indicative of different mechanisms of biochemical homeostasis and different adaptation potential, which should be taken into consideration when prescribing correction.

[The comparative characteristics of the biological and immunomodulating activities of enzyme preparations of microbial origin].

A number of enzyme preparations of microbial origin such as immunomodulators and immunostimulators have been studied in vitro according to reactions of rosette technique of cell (ROC) active and general, adhesion, activation of a complement and also the spontaneous activity and phagocytosis of neutrophils have been determined. The enzyme preparations of microbial origin have been shown to be capable of causing immunomodulation and of influencing the specific and nonspecific immune response. Unlike the enzymes of animal origin the enzymes of microbial origin are effective in smaller concentrations.

[The significance of middle-molecular peptides in the blood in acute forms of ischemic heart disease].

Estimation and analysis of correlations permit disclosing principally new mechanisms in assessment of the homeostasis. The authors have derived curvilinear correlations between medium-molecular peptides, immune complexes, and lipids in coronary patients with the acute forms of the condition. Close relationships (high correlations) between homeostasis parameters were revealed, that were found more manifest in the patients with unstable angina pectoris.

[Various mechanisms of immune homeostasis in myocardial infarction].

Dynamic alterations and interrelationships of the patterns of humoral immunity (immunoglobulins and immune complexes) were studied in 77 patients with myocardium infarction and in 23 healthy donors. Stimulation of immunity homeostasis in the patients with acute form of myocardium infarction was manifested as an increase in content of immunoglobulins and immune complexes. Content of immunoglobulin G was distinctly decreased in epistenocardic pericarditis.

[Lysozyme as a modulator of immunological reactions].

Effect of lysozyme on some immunochemical reactions was studied, involving rosette-developing properties of T lymphocytes and lymphocyte adhesion. The rosette-forming activity of the cells was shown to increase after addition of lysozyme in vitro as well as after treatment with lysozyme in vivo. Participation of lysozyme in activation of complement via various pathways is considered.

[Glycoprotein components of the skin under normal conditions and in several types of pathology].

Content of sialic acids was increased 2.9-3.4-fold in soluble fraction of pathologically altered skin (parakeratotoic scales in psoriasis and allergo dermatoses) as compared with a skin of healthy persons.