Publications by authors named "Doblin Anak Sandai"
Background: A norovirus maintains its viability, infectivity and virulence by its ability to replicate. However, the biological mechanisms of the process remain to be explored. In this work, the NanoLuc™ Luciferase gene was used to develop a reporter-tagged replicon system to study norovirus replication.
View Article and Find Full Text PDFThe chemical nature of most of the mycotoxins makes them highly liposoluble compounds that can be absorbed from the site of exposure such as from the gastrointestinal and respiratory tract to the blood stream where it can be dissimilated throughout the body and reach different organs such as the liver and kidneys. Mycotoxins have a strong tendency and ability to penetrate the human and animal cells and reach the cellular genome where it causes a major mutagenic change in the nucleotide sequence which leads to strong and permanent defects in the genome. This defect will eventually be transcribed, translated and lead to the development of cancer.
View Article and Find Full Text PDFArticle Synopsis
- Traditional diagnosis of leptospirosis relied on immune-seroconversion tests, but new molecular techniques like PCR provide a more accurate detection of the bacteria.
- This study developed a multiplex PCR (mPCR) technique that targets specific genes in Leptospira, successfully testing multiple serovars for heightened detection.
- Results showed the mPCR assay is highly sensitive and can significantly improve early diagnosis of leptospirosis, with the LipL32 gene enhancing the test's effectiveness.