Phytochemical -methyl-L-cysteine sulfoxide from Brassicaceae: a key to health or a poison for bees?

Intensive agricultural practices impact the health and nutrition of pollinators like honey bees (). Rapeseed ( L.) is widely cultivated, providing diverse nutrients and phytochemicals, including -methyl-L-cysteine sulfoxide (SMCSO).

Characterization of secretome during sublethal infection of larvae.

The fungal pathogen can induce prolonged colonization of the lungs of susceptible patients, resulting in conditions such as allergic bronchopulmonary aspergillosis and chronic pulmonary aspergillosis. Analysis of the secretome released during sub-lethal infection of larvae may give an insight into products released during prolonged human colonisation. larvae were infected with and the metabolism of host carbohydrate and proteins and production of fungal virulence factors were analysed.

The Influence of Selected Insecticides on the Oxidative Response of Atta sexdens (Myrmicinae, Attini) Workers.

Reactive oxygen species (ROS) are generated as products of normal cellular metabolic activities; however, the use of pesticides to control leafcutter ants leads to unbalanced ROS production. We evaluated the effects of two insecticides (fipronil, sulfluramid) and metallic insecticide complex (magnesium complex [Mg(hesp)2(phen)] (1)) on the superoxide dismutase (SOD), glutathione (GSH) and the overall antioxidant capacity using two different methodologies: total radical-trapping potential (TRAP) and oxygen radical absorbance capacity (ORAC). Media workers of Atta sexdens (C.

Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. parasitisation and associated factors reveals changes impairing winter bee generation.

The extensive annual loss of honey bees (Apis mellifera L.) represents a global problem affecting agriculture and biodiversity. The parasitic mite Varroa destructor, associated with viral co-infections, plays a key role in this loss.

Numerical and Experimental Analysis of the Load-Carrying Capacity of a Timber Semi-Rigid Dowel-Type Connection.

The paper deals with the analysis of the load-carrying capacity of a timber semi-rigid connection created from a system of two stands and a rung. The connection was made from glued laminated timber with metal mechanical dowel-type fasteners. Not only a common combination of bolts and dowels, but also fully threaded screws were used for the connection.

Numerical and Experimental Analysis of the Rotational Stiffness of a Timber Semi-Rigid Dowel-Type Connection.

The paper deals with the analysis of the rotational stiffness of a semirigid connection created from a system of two stands and a rung. The connection was made from glued laminated timber with metal mechanical dowel-type fasteners. Not only a common combination of bolts and dowels but also fully threaded screws were used for the connection.

Load-Carrying Capacity of Double-Shear Bolted Connections with Slotted-In Steel Plates in Squared and Round Timber Based on the Experimental Testing, European Yield Model, and Linear Elastic Fracture Mechanics.

Nowadays, the use of timber as a building material is gaining more prominence. When designing timber structures, it is necessary to pay increased attention to the design of their connections. The commonly used connections are dowel-type connections, which are often used in combination with steel plates slotted into cut-outs in timber members.

H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees.

In temperate climates, honey bee workers of the species have different lifespans depending on the seasonal phenotype: summer bees (short lifespan) and winter bees (long lifespan). Many studies have revealed the biochemical parameters involved in the lifespan differentiation of summer and winter bees. However, comprehensive information regarding the metabolic changes occurring in their bodies between the two is limited.

Experimental and Numerical Verification of the Railway Track Substructure with Innovative Thermal Insulation Materials.

The article aims to present the modified structural composition of the sub-ballast layers of the railway substructure, in which a part of the natural materials for the establishment of sub-ballast or protective layers of crushed aggregate is replaced by thermal insulation and reinforcing material (layer of composite foamed concrete and extruded polystyrene board). In this purpose, the experimental field test was constructed and the bearing capacity of the modified sub-ballast layers' structure and temperature parameters were analyzed. A significant increase in the original static modulus of deformation on the surface of composite foamed concrete was obtained (3.

Changes in haemolymph parameters and insect ability to respond to immune challenge during overwintering.

Overwintering is a challenging period in the life of temperate insects. A limited energy budget characteristic of this period can result in reduced investment in immune system. Here, we investigated selected physiological and immunological parameters in laboratory-reared and field-collected harlequin ladybirds ().

Winter honeybee () populations show greater potential to induce immune responses than summer populations after immune stimuli.

In the temperate climates of central Europe and North America, two distinct honeybee () populations are found in colonies: short-living summer bees emerge in spring and survive until summer, whereas long-living winter bees emerge in late August and overwinter. Besides the difference in their life spans, each of these populations fulfils a different role in the colonies and individual bees have distinct physiological and immunological adaptations depending on their roles. For instance, winter worker bees have higher vitellogenin levels and larger reserves of nutrients in the fat body than summer bees.

Effects of Loaded End Distance and Moisture Content on the Behavior of Bolted Connections in Squared and Round Timber Subjected to Tension Parallel to the Grain.

This article presents the results of static tests on bolted connections in squared and round timber with inserted steel plates. The experiment evaluates structural timber connections with different distances between the fastener and the loaded end at different moisture contents. Specimens were loaded by tension parallel to the grain and load-deformation diagrams were recorded.

Heptabladed β-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system.

O-methylation is an unusual sugar modification with a function that is not fully understood. Given its occurrence and recognition by lectins involved in the immune response, methylated sugars were proposed to represent a conserved pathogen-associated molecular pattern. We describe the interaction of O-methylated saccharides with two β-propeller lectins, the newly described PLL2 from the entomopathogenic bacterium Photorhabdus laumondii, and its homologue PHL from the related human pathogen Photorhabdus asymbiotica.

Bioactive Excreted/Secreted Products of Entomopathogenic Nematode Inhibit the Phenoloxidase Activity during the Infection.

Entomopathogenic nematodes (EPNs) are efficient insect parasites, that are known for their mutualistic relationship with entomopathogenic bacteria and their use in biocontrol. EPNs produce bioactive molecules referred to as excreted/secreted products (ESPs), which have come to the forefront in recent years because of their role in the process of host invasion and the modulation of its immune response. In the present study, we confirmed the production of ESPs in the EPN , and investigated their role in the modulation of the phenoloxidase cascade, one of the key components of the insect immune system.

The Effect of Foraging on Bumble Bees, , Reared under Laboratory Conditions.

Bumble bees are important pollinators broadly used by farmers in greenhouses and under conditions in which honeybee pollination is limited. As such, bumble bees are increasingly being reared for commercial purposes, which brings into question whether individuals reared under laboratory conditions are fully capable of physiological adaptation to field conditions. To understand the changes in bumble bee organism caused by foraging, we compared the fundamental physiological and immunological parameters of workers reared under constant optimal laboratory conditions with workers from sister colonies that were allowed to forage for two weeks in the field.

Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster.

This study examined how adipokinetic hormone (AKH) and adenosine affect defense responses in Drosophila melanogaster larvae infected with entomopathogenic nematodes (EPN, Steinernema carpocapsae and Heterorhabditis bacteriophora). Three loss-of-function mutant larvae were tested: Akh, AdoR (adenosine receptor), and Akh AdoR. Mortality decreased in all mutants post-EPN infection compared with the control (w).

A Novel Fucose-binding Lectin from Photorhabdus luminescens (PLL) with an Unusual Heptabladed β-Propeller Tetrameric Structure.

Photorhabdus luminescens is known for its symbiosis with the entomopathogenic nematode Heterorhabditis bacteriophora and its pathogenicity toward insect larvae. A hypothetical protein from P. luminescens was identified, purified from the native source, and characterized as an l-fucose-binding lectin, named P.

Rank-based biomarker index to assess cadmium ecotoxicity on the earthworm Eisenia andrei.

A proper soil risk assessment needs to estimate the processes that affect the fate and the behaviour of a contaminant, which are influenced by soil biotic and abiotic components. For this reason, the measurement of biomarkers in soil bioindicator organisms, such as earthworms, has recently received increasing attention. In this study, the earthworm Eisenia andrei was used to assess the pollutant-induced stress syndrome after exposure to sublethal concentrations of Cd (10 or 100 μg g(-1)) in OECD soil, after 14 d of exposure.

Anterior gradient protein 3 is associated with less aggressive tumors and better outcome of breast cancer patients.

Anterior gradient protein (AGR) 3 is a highly related homologue of pro-oncogenic AGR2 and belongs to the family of protein disulfide isomerases. Although AGR3 was found in breast, ovary, prostate, and liver cancer, it remains of yet poorly defined function in tumorigenesis. This study aimed to determine AGR3 expression in a cohort of 129 primary breast carcinomas and evaluate the clinical and prognostic significance of AGR3 in these tumors.

The role of AGR2 and AGR3 in cancer: similar but not identical.

In the past decades, highly related members of the protein disulphide isomerase family, anterior gradient protein AGR2 and AGR3, attracted researchers' attention due to their putative involvement in developmental processes and carcinogenesis. While AGR2 has been widely demonstrated as a metastasis-related protein whose elevated expression predicts worse patient outcome, little is known about AGR3's role in tumour biology. Thus, we aim to confront the issue of AGR3 function in physiology and pathology in the following review by comparing this protein with the better-described homologue AGR2.

Electrocatalytic assay for monitoring methylglyoxal-mediated protein glycation.

Protein glycation is a complex process that plays an important role in diabetes mellitus, aging, and the regulation of protein function in general. As a result, current methodological research on proteins is focused on the development of novel approaches for investigating glycation and the possibility of monitoring its modulation and selective inhibition. In this paper, a first sensing strategy for protein glycation is proposed, based on protein electroactivity measurement.

Is there an intermediate-risk non-seminoma? long-term treatment results from a single center.

Purpose: We retrospectively assessed the treatment results of patients with testicular non-seminoma to evaluate possible predictive and prognostic factors.

Methods: 189 patients with testicular non-seminoma treated between 2000 and 2012 were retrospectively evaluated. Treatment was based on orchiectomy plus chemotherapy (bleomycin/etoposide/cisplatin and vinblastine/ifosfamide/ cisplatin); retroperitoneal lymphadenectomy was only performed for residual disease after chemotherapy.