Development of ultrafast PCR assays for the event-specific detection of eleven approved genetically modified canola events in South Korea.

In Korea, genetically modified (GM) canola events derived from eleven single events have been authorized for food and feed, but not for cultivation. Therefore, the development of a rapid and accurate on-site detection method is crucial for the management of these approved GM canola events. In this study, ultrafast polymerase chain reaction (PCR) assays for the event-specific detection of eleven GM canola events were developed.

Detection of GM Canola MS11, DP-073496-4, and MON88302 events using multiplex PCR coupled with capillary electrophoresis.

As of 2020, 11 GM canola events have been authorized as food for humans in Korea. However, there are no simultaneous multiplex detection methods for 3 GM canola events (DP-073496-4, MON88302, and MS11). Thus, we established the multiplex polymerase chain reaction (PCR) method coupled with capillary electrophoresis to detect 3 GM canola events.

Development of a Systematic qPCR Array for Screening GM Soybeans.

A screening method using the 35S promoter and nos terminator for genetically modified organisms (GMOs) is not sufficient to cover all GM soybean events. In this study, a real-time polymerase chain reaction (also known as quantitative polymerase chain reaction, qPCR) array targeting eight screening assays combined with a prediction system was developed for the rapid tracking of GM soybeans. Each assay's specificity was tested and confirmed using 17 GM soybean events that have been approved in Korea.

Preparation of Porous Carbon Nanofiber Electrodes Derived from 6FDA-Durene/PVDF Blends and Their Electrochemical Properties.

Highly porous carbon electrodes for supercapacitors with high energy storage performance were prepared by using a new precursor blend of aromatic polyimide (PI) and polyvinylidene fluoride (PVDF). Supercapacitor electrodes were prepared through the electrospinning and thermal treatment of the precursor blends of aromatic PI and PVDF. Microstructures of the carbonized PI/PVDF nanofibers were studied using Raman spectroscopy.