Publications by authors named "Dmitry V Lukyanov"
Identification of the proteins specifically binding to the rat LINE1 promoter.
Biochem Biophys Res Commun
February 2006
Article Synopsis
- The dissemination of LINE1 retrotransposons starts with transcription from a specific promoter located in the 5'-untranslated region of LINE1.
- Researchers found that while the rat LINE1 element does have promoter activity, no promoter-binding proteins were previously known.
- Using EMSA and Southwestern blotting methods, they identified two proteins, Sp1 and Sp3, that specifically bind to the rat LINE1 promoter, with the binding motif located downstream of the main transcription initiation site.
Analysis of polymorphic sites in the promoter of the nitric oxide synthase 2 gene.
Valentina Coia Simone Jüliger Benjamin Mordmüller Andrea Kreidenweis Anna-Lena Stroh Dmitry V Lukyanov
Biochem Biophys Res Commun
October 2005
A point mutation (G --> C) in the gene promoter for the human nitric oxide synthase (NOS) 2 at position -954 is associated with protection against severe Plasmodium falciparum malaria in Gabon. Carriers of this mutation show higher basal levels of nitric oxide production than wild type individuals. To obtain information about the possible binding transcription factors, nucleic proteins from the lung carcinoma cell line were enriched by affinity chromatography using DEAE-Sepharose and immobilized oligonucleotides derived from the promoter sequence.
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