Integration of Flow Cytometry and Single Cell Sequencing.

Integrating cytometric analysis of cells, mitochondria, and other polynucleotide-containing biological particles with high-throughput single particle sequencing would provide an ultimate bioanalytical tool, simultaneously assessing phenotype, functionality, genome, and transcriptome of each particle in a large population. Here, we describe how such integration could be performed by adapting existing, well-established technologies.

Fabrication of perforated sub-micron silica shells.

A new synthetic approach for fabrication of perforated hollow silica morphologies using colloidal template assemblies is demonstrated. As proof-of-principle, the polystyrene/silver colloidal assemblies had chemically modified surfaces. The template dissolution resulted in the fabrication of the submicron perforated hollow silica shells.