Image-seq: spatially resolved single-cell sequencing guided by in situ and in vivo imaging.

Tissue function depends on cellular organization. While the properties of individual cells are increasingly being deciphered using powerful single-cell sequencing technologies, understanding their spatial organization and temporal evolution remains a major challenge. Here, we present Image-seq, a technology that provides single-cell transcriptional data on cells that are isolated from specific spatial locations under image guidance, thus preserving the spatial information of the target cells.

Laser Micromachining of Bone as a Tool for Studying Bone Marrow Biology.

The bone marrow (BM) has traditionally been a difficult tissue to access because it is embedded deep within the bone matrix. It is home to the hematopoietic stem cells (HSCs) that give rise to all blood cells in the body. It is also the site of origin for malignant blood cells such as leukemia and multiple myeloma, as well as a frequent site of metastasis for many solid tumors including prostate and breast cancer.

Cerebrospinal fluid can exit into the skull bone marrow and instruct cranial hematopoiesis in mice with bacterial meningitis.

Interactions between the immune and central nervous systems strongly influence brain health. Although the blood-brain barrier restricts this crosstalk, we now know that meningeal gateways through brain border tissues facilitate intersystem communication. Cerebrospinal fluid (CSF), which interfaces with the glymphatic system and thereby drains the brain's interstitial and perivascular spaces, facilitates outward signaling beyond the blood-brain barrier.

From coffee stains to uniform deposits: Significance of the contact-line mobility.

Hypothesis: Contact-line motion upon drying of a sessile droplet strongly affects the solute transport and solvent evaporation profile. Hence, it should have a strong impact on the deposit formation and might be responsible for volcano-like, dome-like and flat deposit morphologies.

Experiments: A method based on a thin-film interference was used to track the drop height profile and contact line motion during the drying.

Relative retinal flow velocity detection using optical coherence tomography angiography imaging.

Optical coherence tomography angiography (OCTA) imaging is a valuable tool for the visualization of retinal vasculature at an unprecedented level of details. However, due to relatively long time-interval between repeated scans in the conventional OCTA scanning protocol, the OCTA flow signal suffers from low dynamic range and loss of velocity-intensity correlation. The ability to distinguish fast and slow flow in the retina may provide a powerful tool for the assessment of early-stage retinal diseases such as vein occlusion.

Optical-resonance-assisted generation of super monodisperse microdroplets and microbeads with nanometer precision.

Droplets with predefined sizes have been controllably produced at the tip of a micro-capillary immersed in an external fluid while tracking the high Q-factor whispering gallery modes (WGM). The modes were fitted to a model to give precise real-time size measurement, which was used as a feedback to control the pressure in the capillary and the release of the droplet from the capillary when it reached the target size. In this way a dispersion of highly monodisperse droplets anywhere in the size range from 5 μm to 50 μm were produced.

Imaging mechanical properties of sub-micron ECM in live zebrafish using Brillouin microscopy.

In this work, we quantify the mechanical properties of the extra-cellular matrix (ECM) in live zebrafish using Brillouin microscopy. Optimization of the imaging conditions and parameters, combined with careful spectral analysis, allows us to resolve the thin ECM and distinguish its Brillouin frequency shift, a proxy for mechanical properties, from the surrounding tissue. High-resolution mechanical mapping further enables the direct measurement of the thickness of the ECM label-free and .