Mini-Multilocus Sequence Typing Scheme for the Global Population of .

The increasing problem of antimicrobial resistance in necessitates the development of molecular typing schemes that are suitable for rapid and mass screening. The objective of this study was to design and validate a mini-MLST scheme for based on global pathogen population data. Using sequences of seven housekeeping genes of 21,402 isolates with known MLSTs from the PubMLST database, we identified eighteen informative polymorphisms and obtained mini-MLST nucleotide profiles to predict MLSTs of isolates.

Gonococcal Genetic Island in the Global Population: A Model of Genetic Diversity and Association with Resistance to Antimicrobials.

The aim of this work was to study the genetic diversity of the gonococcal genetic island (GGI) responsible for the type IV secretion system (T4SS) and the association of a functionally active GGI with antimicrobial resistance. An analysis of the GGI in a sample of 14,763 genomes of isolates from the Pathogenwatch database collected in 1996-2019 from 68 countries was performed. A model of GGI's genetic diversity that divides the global gonococcal population into fifty-one GGI clusters and three GGI superclusters based on the allele type of the gene and substitutions of the and genes for and has been proposed, reflecting differences among isolates in the T4SS functionality.

Azithromycin Susceptibility Testing and Molecular Investigation of Isolates Collected in Russia, 2020-2021.

The aim of this work was to study the resistance to macrolides (azithromycin) in the modern Russian population of with the analysis of genetic resistance determinants. Azithromycin is not used to treat gonococcal infection in Russia. However, among 162 isolates collected in 2020-2021, 22 isolates (13.

Genomic Diversity and Chromosomal Rearrangements in and .

Chromosomal rearrangements in and were studied with the determination of mobile elements and their role in rearrangements. The results of whole-genome sequencing and de novo genome assembly for 50 collected in Russia were compared with 96 genomes of and 138 genomes of from the databases. Rearrangement events with the determination of the coordinates of syntenic blocks were analyzed using the SibeliaZ software v.

Comparative Whole-Genome Analysis of Isolates Revealed Changes in the Gonococcal Genetic Island and Specific Genes as a Link to Antimicrobial Resistance.

Comparative whole-genome analysis was performed for isolates belonging to the multiantigen sequence typing (NG-MAST) types predominant worldwide - 225, 1407, 2400, 2992, and 4186 - and to genogroup 807, the most common genogroup in the Russian Federation. Here, for the first time, the complete genomes of 25 isolates from genogroup 807 were obtained. For NG-MAST types 225, 1407, 2400, 2992, and 4186, genomes from the Pathogenwatch database were used.

Hydrogel Droplet Microarray for Genotyping Antimicrobial Resistance Determinants in Isolates.

A multiplex assay based on a low-density hydrogel microarray was developed to identify genomic substitutions in that determine resistance to the currently recommended treatment agents ceftriaxone and azithromycin and the previously used drugs penicillin, tetracycline, and ciprofloxacin. The microarray identifies 74 drug resistance determinants in the  , , , , , , , , , and 23S rRNA genes. The hydrogel elements were formed by automated dispensing of nanoliter-volume droplets followed by UV-induced copolymerization of NH-containing oligonucleotides with gel-forming monomers.

Prediction of ceftriaxone MIC in Neisseria gonorrhoeae using DNA microarray technology and regression analysis.

Background: Decreased susceptibility of Neisseria gonorrhoeae to extended-spectrum cephalosporins is a major concern. Elucidation of the phenotypic and genetic characteristics of such isolates is a priority task.

Methods: We developed a method for predicting the N.

Molecular Typing of Clinical Isolates in Russia, 2018-2019: A Link Between Alleles and NG-MAST Types.

This work aimed to study gene polymorphisms in clinical isolates of collected in Russia in 2018-2019 and the contribution of the allele type to susceptibility to β-lactam antibiotics. A total of 182 isolates were analyzed. allele types were determined by sequencing, and the minimum inhibitory concentrations (MICs) of benzylpenicillin and ceftriaxone were measured.