Genomic deletions in based on transformation of linear heterologous DNA.

Several genome engineering methods have been developed for . However, they suffer from limitations such as extensive cloning, multiple steps, successful expression of heterologous genes via plasmid etc. Here, we report a rapid method for performing genomic deletions/disruptions in spp.

Role of Bacterial Cytoskeleton and Other Apparatuses in Cell Communication.

The bacterial cytoskeleton is crucial for sensing the external environment and plays a major role in cell to cell communication. There are several other apparatuses such as conjugation tubes, membrane vesicles, and nanotubes used by bacterial cells for communication. The present review article describes the various bacterial cytoskeletal proteins and other apparatuses, the physical structures they form and their role in sensing environmental stress.

How similar or dissimilar cells are produced by bacterial cell division?

DNA replication, segregation and cell division are vital processes and require an interplay of multiple proteins. These processes are highly conserved across bacteria yet similar or dissimilar progeny are produced after cell division. This review describes the bacterial cell division in considerable detail.

Is Different from Other Members of : Monoploidy, Overlapping Replication Cycle, and Unique Segregation Pattern.

Among actinomycetes, chromosome organization and segregation studies have been limited to , , and spp. There are differences with respect to ploidy and chromosome organization pattern in these bacteria. Here, we report on chromosome replication, organization, and segregation in PR4, which has a circular genome of 6.

Deletion of pyruvate decarboxylase gene in Zymomonas mobilis by recombineering through bacteriophage lambda red genes.

Zymomonas mobilis ZM4 is a gram negative ethanologenic bacterium used in several biotechnological applications. Metabolic engineering in this bacterium is limited because of the available genome engineering tools. In the present study, we report genome engineering in this bacterium using bacteriophage lambda Red genes.

Localization of Low Copy Number Plasmid pRC4 in Replicating Rod and Non-Replicating Cocci Cells of Rhodococcus erythropolis PR4.

Rhodococcus are gram-positive bacteria, which can exist in two different shapes rod and cocci. A number of studies have been done in the past on replication and stability of small plasmids in this bacterium; however, there are no reports on spatial localization and segregation of these plasmids. In the present study, a low copy number plasmid pDS3 containing pRC4 replicon was visualized in growing cells of Rhodococcus erythropolis PR4 (NBRC100887) using P1 parS-ParB-GFP system.

Isolation and molecular characterization of a stationary phase promoter useful for gene expression in Gordonia.

Gordonia are gram-positive bacteria belonging to Actinomycetes family with a wide variety of industrial and environmental applications. The genetic toolbox, however, is limited for manipulation of these organisms. In the present study, a new promoter has been isolated from Gordonia sp.