Publications by authors named "Divya Chalapathi"
Conventional methods of detecting economically essential mutations have several disadvantages. Even though fluorescence-based methods are still the best option, Surface-Enhanced Raman Spectroscopy (SERS) may soon emerge as an alternative to the current techniques for detecting these mutations, because of its ability to detect molecular vibrational signatures. We were able to identify and develop a PCR-based SERS assay that can relentlessly differentiate between different types of indels, and SNPs as demonstrated in the case of tomato genome related to tomato yellow leaf curl virus and root-knot nematodes, diseases that are economically significant to the global agriculture industry and where the selection of resistant crops is the best solution.
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- This study investigates how hen egg-white lysozyme (HEWL) aggregates at a high pH of 12.2, which is important for understanding neurodegenerative diseases where protein misfolding occurs.
- Researchers found that as HEWL aggregates, β sheets form, impacting the protein's structure, and discovered that disulphide bonds between protein monomers play a key role in this process.
- The use of techniques like Molecular Dynamics simulations and protein docking helped visualize these changes, showing that dimer formation is favored at high pH compared to neutral conditions.
Extracellular vesicles (EVs) laden with lipids, proteins, DNA, and micro-RNAs play important biological functions in intercellular communication and have pivotal roles in pathophysiological conditions. Characterization of the EVs has always been a multistep process involving large volumes, and they are heterogeneous in size and properties. A multitude of approaches is used to distinguish the EVs.
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