Crop rotation and inoculation increase soil bradyrhizobia population, soybean grain yields, and profitability.

Crop rotation and rhizobial inoculation are strategies to increase yield by means of organic matter addition and modulation of microbial diversity. However, the extent to which these agricultural practices change soil Bradyrhizobium populations, soybean grain yield, and economic benefits to farmers is unclear. Thus, this study aimed to evaluate the interaction between crop rotation and inoculation of soybean (Glycine max) cultivated in two contrasting soils (clayey and sandy soil) on biological nitrogen fixation components, grain yields, and profits.

Microalgae cultivation in wastewater from agricultural industries to benefit next generation of bioremediation: a bibliometric analysis.

The aim of this study was to provide a bibliometric analysis and mapping of existing scientific papers, focusing on microalgae cultivation coupled with biomass production and bioremediation of wastewater from agricultural industries, including cassava, dairy, and coffee. Using the Web of Science (WoS) database for the period 1996-2021, a search was performed using a keyword strategy, aiming at segregating the papers in groups. For the first search step, the keywords "wastewater treatment", AND "microalgae", AND "cassava" OR "dairy" OR "coffee" were used, resulting in 59 papers.

Active projects for teaching and learning soil microbiology and applications of inoculants to increase perceived subject matter understanding and acquisition of knowledge.

Active methodologies for teaching propose tools and strategies for improving student learning by using participative and integrative approaches. These lead students to autonomous research for industry problems and solutions. This study aimed to apply active-project active methodologies to undergraduate soil microbiology and inoculant courses to verify students' perception of their knowledge levels on these topics.

Combining glucose and sodium acetate improves the growth of Neochloris oleoabundans under mixotrophic conditions.

Mixotrophic cultivation is a potential approach to produce microalgal biomass that can be used as raw materials for renewable biofuels and animal feed, although using a suitable, cost-effective organic carbon source is crucial. Here, we used a Box-Behnken design with three factors, the glucose and sodium acetate concentrations, and the percentage of Bold's basal medium (BBM), to evaluate the effects of different carbon sources on biomass productivity and the protein and lipid contents of Neochloris oleoabundans (UTEX#1185). When grow at optimal levels of these factors, 100 % BBM plus 7.

Rhizobium paranaense sp. nov., an effective N2-fixing symbiont of common bean (Phaseolus vulgaris L.) with broad geographical distribution in Brazil.

Nitrogen (N), the nutrient most required for plant growth, is key for good yield of agriculturally important crops. Common bean (Phaseolus vulgaris L.) can benefit from bacteria collectively called rhizobia, which are capable of fixing atmospheric nitrogen (N2) in root nodules and supplying it to the plant.

Rhizobium freirei sp. nov., a symbiont of Phaseolus vulgaris that is very effective at fixing nitrogen.

Common bean (Phaseolus vulgaris L.) can establish symbiotic associations with several Rhizobium species; however, the effectiveness of most strains at fixing nitrogen under field conditions is very low. PRF 81(T) is a very effective strain, usually referred to as Rhizobium tropici and used successfully in thousands of doses of commercial inoculants for the common bean crop in Brazil; it has shown high rates of nitrogen fixation in all areas representative of the crop in the country.

Proteomic profiling of Rhizobium tropici PRF 81: identification of conserved and specific responses to heat stress.

Background: Rhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of N-fertilizers. The objective of our study was to obtain an overview of adaptive responses to heat stress of strain PRF 81, by analyzing differentially expressed proteins when the bacterium is grown at 28°C and 35°C.

Results: Two-dimensional gel electrophoresis (2DE) revealed up-regulation of fifty-nine spots that were identified by MALDI-TOF/TOF-TOF.

Two-dimensional proteome reference map of Rhizobium tropici PRF 81 reveals several symbiotic determinants and strong resemblance with agrobacteria.

Rhizobium tropici strain PRF 81 is used in commercial inoculants for common-bean crops in Brazil because of its high efficiency in nitrogen fixation and, as in other strains belonging to this species, its tolerance of environmental stresses, representing a useful biological alternative to chemical nitrogen fertilizers. In this study, a proteomic reference map of PRF 81 was obtained by two-dimensional gel electrophoresis and MALDI-TOF/TOF-TOF mass spectrometry. In total, 115 spots representing 109 different proteins were successfully identified, contributing to a better understanding of the rhizobia-legume symbiosis and supporting, at proteomics level, a strong resemblance with agrobacteria.