Embryonic Toxicology Evaluation of Ginger- and Clove-mediated Titanium Oxide Nanoparticles-based Dental Varnish with Zebrafish.

Aim: The aim of the study is to evaluate the embryonic toxicology of ginger- and clove-mediated titanium oxide (TiO) nanoparticles (NPs)-based dental varnish with zebrafish ().

Materials And Methods: Dental varnish was formulated using ginger, clove extract, and titanium dioxide NPs followed by the introduction of this test solution at concentrations of 1, 2, 4, 8, and 16 µL along with a control group with medium zebrafish embryos into a 6-well culture plate. After 2 hours of incubation, the embryos of zebrafish were tested and analyzed for hatchability and mortality rate using one-way ANOVA and Tukey's tests using statistical package for the social sciences (SPSS) software.