Detection of Tomato Leaf Curl New Delhi Virus-ES by Real-Time Quantitative PCR.

The tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite, single-stranded DNA begomovirus (genus Begomovirus, family Geminiviridae) that was first identified in India in 1995 affecting solanaceous cucurbitaceous crops in India. In 2012 a new virus strain, denominated ToLCNDV-ES, was first detected in Europe affecting zucchini squash, melon, cucumber, and to a lesser degree, tomato, in Spain and other Mediterranean countries. The virus is currently listed as a quarantine pest by EPPO.

-Specific DNA in Domestic and Synanthropic Vertebrate Insectivore Feces.

The ecology of greenhouse pests generally involves parasitoid or predatory insects. However, we investigated whether the leaf miner (Meyrick, 1917) (Lepidoptera: Gelechiidae) is part of the diet of domestic and synanthropic vertebrate animals, such as birds, reptiles, and mammals, and that take part in an ecosystem that contains a high density of tomato greenhouses. Feces from domesticated partridges, common quails, and chickens, as well as from wild lizards were collected within tomato greenhouses, and fecal pellets from bats, swallows, common swifts, and house martins living in the vicinity of tomato greenhouses were collected outside.

Exogenous Application of dsRNA for the Control of Viruses in Cucurbits.

The recurrent emergence of viral diseases in intensive horticultural crops requires alternative control strategies. The topical application of double-stranded RNA (dsRNA) molecules homologous to pathogens has been proposed as a tool for virus control in plants. These dsRNAs induce the silencing mechanism, the RNA interference (RNAi), that degrades homologous dsRNAs.

Host Species-Dependent Transmission of Tomato Leaf Curl New Delhi Virus-ES by .

The tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite, single-stranded begomovirus that was first identified in India in 1995 affecting solanaceous crops. A different strain, named ToLCNDV-ES, was introduced in Spain in 2012 and causes severe symptoms in zucchini crops. Virus transmission experiments with the whitefly , were used to compare the transmission parameters in zucchini and tomato plants.

Special Issue: "Plant Virus Epidemiology".

We are pleased to present this Special Issue on the topic of "Plant virus Epidemiology" [...

Resistance to Cucumber Green Mottle Mosaic Virus in .

Cucumber green mottle mosaic virus (CGMMV) is a severe threat to melon production worldwide. At present, there are no cultivars available on the market which show an effective resistance or tolerance to CGMMV infection; only wild species were reported as resistant. Germplasm accessions of , as well as , , and were mechanically infected with isolates belonging to the European and Asian strain of CGMMV and screened for resistance by scoring symptom severity and comparing the accumulation of virus by qRT-PCR.

Epidemiology and control of emerging criniviruses in bean.

During the last two decades, new criniviruses emerged in green bean crops in the south-east of Spain. Bean yellow disorder virus (BnYDV) was first detected in 2003 and caused major economic damage in crops grown in greenhouses. It was characterized as the first crinivirus to infect a member species of the Leguminosae family.

Whitefly Control Strategies against Tomato Leaf Curl New Delhi Virus in Greenhouse Zucchini.

(1) Tomato leaf curl New Delhi virus (ToLCNDV), transmitted by tobacco whitefly ( Gennadius) (Hemiptera: Aleyrodidae), is of major concern in the cultivation of zucchini. The threat of this virus motivates reliance on chemical vector control but European consumers' demands for vegetables grown free of pesticides provides an important incentive for alternative pest management; (2) Different whitefly management strategies and ToLCNDV incidences were surveyed in commercial zucchini greenhouses in south-east Spain. In an experimental greenhouse, three different whitefly control strategies, biological, chemical, and integrated (IPM), were evaluated in a replicated trial to determine the most effective strategy for virus suppression (3) Whitefly was present in all commercial zucchini crops surveyed, whereas fewer crops had or other natural enemies.

Absolute Quantification of Tomato leaf curl New Delhi virus Spain strain, ToLCNDV-ES: Virus Accumulation in a Host-Specific Manner.

Tomato leaf curl New Delhi virus (ToLCNDV) (family Geminiviridae, genus Begomovirus) has recently been introduced in western Mediterranean countries. Isolates in Spain constitute a new strain, denominated ToLCNDV-ES, that is causing losses in commercial zucchini and melon crops; however, it is also, although less often, detected in commercial tomato crops. We developed a tissue-print hybridization test to detect the two genomic components of the virus and a TaqMan quantitative polymerase chain reaction (qPCR) test to estimate the number of genome copies in plants.

First natural crossover recombination between two distinct species of the family Closteroviridae leads to the emergence of a new disease.

Lettuce chlorosis virus-SP (LCV-SP) (family Closteroviridae, genus Crinivirus), is a new strain of LCV which is able to infect green bean plants but not lettuce. In the present study, high-throughput and Sanger sequencing of RNA was used to obtain the LCV-SP full-length sequence. The LCV-SP genome comprises 8825 nt and 8672 nt long RNA1 and RNA2 respectively.

Biological and Molecular Diversity of Cucumber green mottle mosaic virus in Spain.

The complete RNA genome from Cucumber green mottle mosaic virus (CGMMV) (Alm08), collected during 2009 in cucumber crops located in Spain, was found to be 6,422 nucleotides long. The nucleotide sequence shared the highest identity with isolates from Russia (GQ495274, GQ495275, FJ848666) as do nucleotide sequences of partial CP and MP genes described in Spain since 2005. All the partial genome sequences including RdRp, CP, and MP from 26 isolates collected from 2013 to 2015 in the southeast of Spain, and from seven isolates of other parts of the world, suggest that they grouped in two major clusters: one cluster (I) included 14 isolates collected between 2013 and 2014, and also reference isolates from France, the Netherlands, and Uzbekistan.

Optimal Strategies for Interception, Detection, and Eradication in Plant Biosecurity.

The introduction of invasive species causes damages from the economic and ecological point of view. Interception of plant pests and eradication of the established populations are two management options to prevent or limit the risk posed by an invasive species. Management options generate costs related to the interception at the point of entry, and the detection and eradication of established field populations.

Countering countermeasures: detecting identity lies by detecting conscious breakthrough.

One major drawback of deception detection is its vulnerability to countermeasures, whereby participants wilfully modulate their physiological or neurophysiological response to critical guilt-determining stimuli. One reason for this vulnerability is that stimuli are usually presented slowly. This allows enough time to consciously apply countermeasures, once the role of stimuli is determined.

Subliminal salience search illustrated: EEG identity and deception detection on the fringe of awareness.

We propose a novel deception detection system based on Rapid Serial Visual Presentation (RSVP). One motivation for the new method is to present stimuli on the fringe of awareness, such that it is more difficult for deceivers to confound the deception test using countermeasures. The proposed system is able to detect identity deception (by using the first names of participants) with a 100% hit rate (at an alpha level of 0.

Yellowing disease in zucchini squash produced by mixed infections of Cucurbit yellow stunting disorder virus and Cucumber vein yellowing virus.

Zucchini squash is host to Cucurbit yellow stunting disorder virus (CYSDV), a member of the genus Crinivirus, and Cucumber vein yellowing virus (CVYV), a member of the genus Ipomovirus, both transmitted by the whitefly Bemisia tabaci. Field observations suggest the appearance of new symptoms observed on leaves of zucchini squash crops when both viruses were present. When infected during controlled experiments with CYSDV only, zucchini plants showed no obvious symptoms and the virus titer decreased between 15 and 45 days postinoculation (dpi), after which it was no longer detected.

Twice random, once mixed: applying mixed models to simultaneously analyze random effects of language and participants.

Psychologists, psycholinguists, and other researchers using language stimuli have been struggling for more than 30 years with the problem of how to analyze experimental data that contain two crossed random effects (items and participants). The classical analysis of variance does not apply; alternatives have been proposed but have failed to catch on, and a statistically unsatisfactory procedure of using two approximations (known as F(1) and F(2)) has become the standard. A simple and elegant solution using mixed model analysis has been available for 15 years, and recent improvements in statistical software have made mixed models analysis widely available.

Switching Modalities in A Sentence Verification Task: ERP Evidence for Embodied Language Processing.

In an event related potential (ERP) experiment using written language materials only, we investigated a potential modulation of the N400 by the modality switch effect. The modality switch effect occurs when a first sentence, describing a fact grounded in one modality, is followed by a second sentence describing a second fact grounded in a different modality. For example, "A cellar is dark" (visual), was preceded by either another visual property "Ham is pink" or by a tactile property "A mitten is soft.

Perfectionism and coping with daily failures: positive reframing helps achieve satisfaction at the end of the day.

Differentiating perfectionistic strivings and perfectionistic concerns, the present study examined how perfectionism predicts what coping strategies people use, when dealing with failures, and how perfectionism and coping influence people's satisfaction. A sample of 149 students completed daily reports for 3-14 days, reporting the most bothersome failure they experienced during the day, what strategies they used to cope with the failure, and how satisfied they felt at the end of the day. Multilevel regression analyses showed that perfectionistic concerns predicted more frequent use of self-blame, less frequent use of active coping and acceptance, and higher satisfaction at the end of the day, whereas perfectionistic strivings predicted less frequent use of self-blame and higher satisfaction.

Triple pelvic osteotomy as treatment for osteoarthritis secondary to developmental dysplasia of the hip.

Joint-preserving osteotomies are an established treatment for adult hip pain secondary to developmental dysplasia of the hip. However, their value for advanced osteoarthritis is unclear. Therefore this study addresses the question of long-term results of triple pelvic osteotomy in patients with second grade osteoarthritis.

Tumor sclerosis but not cell proliferation or malignancy grade is a prognostic marker in advanced-stage follicular lymphoma: the German Low Grade Lymphoma Study Group.

Purpose: Follicular lymphoma is an indolent lymphoma with a long median overall survival. However, a considerable number of patients die within the first 2 years after the onset of the disease. Because the treatment options vary with respect to antitumor effect and potential toxic adverse effects, the identification of high-risk patients would be helpful in directing therapeutic decisions in individual patients.

Gender differences in cooperation and competition: the male-warrior hypothesis.

Evolutionary scientists argue that human cooperation is the product of a long history of competition among rival groups. There are various reasons to believe that this logic applies particularly to men. In three experiments, using a step-level public-goods task, we found that men contributed more to their group if their group was competing with other groups than if there was no intergroup competition.

Low genetic diversity among Cucumber vein yellowing virus isolates from Spain.

The population structure and genetic diversity of Cucumber vein yellowing virus (CVYV) from Spain were estimated by analyses of partial nucleotide sequences of the P1-proteinase (P1-Pro), P3 protein (P3), and the coat protein (CP) coding regions. Analysis of 56 CVYV Spanish field isolates collected from 2001 to 2005 showed low genetic diversity (0.0026, 0.

Clonal nature of sclerosing polycystic adenosis of salivary glands demonstrated by using the polymorphism of the human androgen receptor (HUMARA) locus as a marker.

Sclerosing polycystic adenosis (SPA) is a recently described, rare lesion of the salivary glands that bears a resemblance to epithelial proliferative lesions of the breast. The true nature of the lesion is unknown, but up to now it has been generally believed to represent a pseudoneoplastic sclerosing and inflammatory process. However, local recurrence developed in about one-third of the cases.

Impact of cranial radiotherapy on central nervous system prophylaxis in children and adolescents with central nervous system-negative stage III or IV lymphoblastic lymphoma.

Purpose: In the Non-Hodgkin's Lymphoma-Berlin-Frankfurt-Munster (NHL-BFM) 95 trial, we tested, against the historical control of the combined trials NHL-BFM90 and NHL-BFM86, whether prophylactic cranial radiotherapy (PCRT) can be omitted for CNS-negative patients with stage III or IV lymphoblastic lymphoma (LBL) with sufficient early response.

Patients And Methods: Apart from the removal of PCRT in NHL-BFM95, the chemotherapy of the three trials was identical except for the amount of l-asparaginase and daunorubicin during induction. The therapy in NHL-BFM95 was accepted to be noninferior when compared with trials NHL-BFM90/86 if the lower limit of the one-sided 95% CI for the difference in the 2-year probability of event-free-survival (pEFS) between target patients of NHL-BFM95 and the historical controls of NHL-BFM90/86 did not exceed -14%.