Transcriptional bursts and heterogeneity among cardiomyocytes in hypertrophic cardiomyopathy.

Transcriptional bursting is a common expression mode for most genes where independent transcription of alleles leads to different ratios of allelic mRNA from cell to cell. Here we investigated burst-like transcription and its consequences in cardiac tissue from Hypertrophic Cardiomyopathy (HCM) patients with heterozygous mutations in the sarcomeric proteins cardiac myosin binding protein C (cMyBP-C, ) and cardiac troponin I (cTnI, ). Using fluorescence hybridization (RNA-FISH) we found that both, and are transcribed burst-like.