Understanding resource use and dietary niche partitioning in a high-altitude predator guild using seasonal sampling and DNA metabarcoding.

Understanding of predator feeding ecology, interactions among co-occurring predator species, and seasonal changes is critical for conservation management given the important role that predators play in shaping their ecosystems, but is lacking for most regions of the world. Dietary studies have demonstrated varying conclusions in the role that resource partitioning plays in the maintenance of predator communities due to complex inter-related factors that may shape prey use. We used DNA metabarcoding on 581 scat samples to determine the dietary composition, similarity, diversity, and niche overlap of eight predator species (Tibetan wolf (Canis lupus), snow leopard (Panthera uncia), Tibetan brown bear (Ursus arctos pruinosus), Eurasian lynx (Lynx lynx), Tibetan fox (Vulpes ferrilata), red fox (V.

Temporal and Spatial Activity Patterns of Sympatric Wild Ungulates in Qinling Mountains, China.

Dramatic increases in populations of wild ungulates have brought a new ecological issue in the Qinling mountains. Information on species' niche differentiation will contribute to a greater understanding of the mechanisms of coexistence, so as to ultimately benefit the conservation and management of ecological communities. In this study, camera trapping was used to investigate spatial and temporal activity patterns of sympatric wild ungulates in the Qinling Mountains of China, where top predators were virtually absent.

Mitigation Strategies for Human-Tibetan Brown Bear () Conflicts in the Hinterland of the Qinghai-Tibetan Plateau.

Personal injury and property damage caused by wildlife can worsen the relationship between humans and wildlife. In recent years, conflicts between herders and Tibetan brown bears () (human-bear conflicts; HBCs) on the Qinghai-Tibetan Plateau have increased dramatically, severely affecting community motivation for the conservation of brown bears and other species. Understanding the types, effectiveness, and flaws of current HBC mitigation measures is critical to develop effective strategies to alleviate HBC.

Spatial-Temporal Patterns of Sympatric Asiatic Black Bears () and Brown Bears () in Northeastern China.

Studying the spatial and temporal interactions between sympatric animal species is essential for understanding the mechanisms of interspecific coexistence. Both Asiatic black bears () and brown bears () inhabit northeastern China, but their spatial-temporal patterns and the mechanism of coexistence were unclear until now. Camera traps were set in Heilongjiang Taipinggou National Nature Reserve (TPGNR) from January 2017 to December 2017 to collect photos of the two sympatric bear species.

Projected impacts of climate change on snow leopard habitat in Qinghai Province, China.

Assessing species' vulnerability to climate change is a prerequisite for developing effective strategies to reduce emerging climate-related threats. We used the maximum entropy algorithm (MaxEnt model) to assess potential changes in suitable snow leopard () habitat in Qinghai Province, China, under a mild climate change scenario. Our results showed that the area of suitable snow leopard habitat in Qinghai Province was 302,821 km under current conditions and 228,997 km under the 2050s climatic scenario, with a mean upward shift in elevation of 90 m.

The complete chloroplast genome sequences of (Cornaceae).

is a species of the , an evergreen tree endemic to China. Here, we report a complete chloroplast genome sequence of . The chloroplast genome was found to be 157,400bp in length, and G-C contents were 38.

Implementing a comprehensive approach to study the causes of human-bear (Ursus arctos pruinosus) conflicts in the Sanjiangyuan region, China.

Personal injury and property loss caused by wildlife often deteriorates the relationship between humans and animals, prompting retaliatory killings that threaten species survival. Conflicts between humans and Tibetan brown bears (Ursus arctos pruinosus) (Human-Bear Conflicts, HBC) in the Sanjiangyuan region have recently dramatically increased, seriously affecting community enthusiasm for brown bears and the conservation of other species. In order to understand the driving mechanisms of HBC, we proposed six potential drivers leading to increased occurrences of HBC.

Spatial and temporal activity patterns of Golden takin () recorded by camera trapping.

Understanding animals' migration, distribution and activity patterns is vital for the development of effective conservation action plans; however, such data for many species are lacking. In this study, we used camera trapping to document the spatial and temporal activity patterns of golden takins () in Changqing National Nature Reserve in the Qinling mountains, China, from April 2014 to October 2017. Our study obtained 3,323 independent detections (from a total of 12,351 detections) during a total camera trapping effort of 93,606 effective camera trap days at 573 sites.

Identifying the risk regions of house break-ins caused by Tibetan brown bears () in the Sanjiangyuan region, China.

Damage to homesteads by brown bears () has become commonplace in Asia, Europe, and the Americas. Science-based solutions for preventing damages can contribute to the establishment of mechanisms that promote human-bear coexistence. We examined the spatial distribution patterns of house break-ins by Tibetan brown bears () in Zhiduo County of the Sanjiangyuan region in China.

Identifying climate refugia and its potential impact on Tibetan brown bear () in Sanjiangyuan National Park, China.

Climate change has direct impacts on wildlife and future biodiversity protection efforts. Vulnerability assessment and habitat connectivity analyses are necessary for drafting effective conservation strategies for threatened species such as the Tibetan brown bear (). We used the maximum entropy (MaxEnt) model to assess the current (1950-2000) and future (2041-2060) habitat suitability by combining bioclimatic and environmental variables, and identified potential climate refugia for Tibetan brown bears in Sanjiangyuan National Park, China.

Genetic diversity of the Sichuan snub-nosed monkey (Rhinopithecus roxellana) in Shennongjia National Park, China using RAD-seq analyses.

The Sichuan snub-nosed monkey (Rhinopithecus roxellana) is an endangered species endemic to China, where the smallest population resides in Shennongjia National Park, Hubei Province. In this study, the genetic diversity of the Sichuan snub-nosed monkeys from two areas, Qianjiaping (QJP) and Dalongtan (DLT) in Shennongjia National Park was evaluated using single nucleotide polymorphisms (SNPs) data derived from restriction site-associated DNA sequencing (RAD-seq). A total of 41,260 SNP loci were found in 29 Sichuan snub-nosed monkey individuals.

Broad-leaved forest types affect soil fungal community structure and soil organic carbon contents.

Evergreen broad-leaved (EBF) and deciduous broad-leaved (DBF) forests are two important vegetation types in terrestrial ecosystems that play key roles in sustainable biodiversity and global carbon (C) cycling. However, little is known about their associated soil fungal community and the potential metabolic activities involved in biogeochemical processes. In this study, soil samples were collected from EBF and DBF in Shennongjia Mountain, China, and soil fungal community structure and functional gene diversity analyzed based on combined Illumina MiSeq sequencing with GeoChip technologies.

Identifying refugia and corridors under climate change conditions for the Sichuan snub-nosed monkey () in Hubei Province, China.

Using a case study of an isolated management unit of Sichuan snub-nosed monkey (), we assess the extent that climate change will impact the species' habitat distribution in the current period and projected into the 2050s. We identify refugia that could maintain the population under climate change and determine dispersal paths for movement of the population to future suitable habitats. Hubei Province, China.

Identifying potential refugia and corridors under climate change: A case study of endangered Sichuan golden monkey (Rhinopithecus roxellana) in Qinling Mountains, China.

Climate change threatens endangered species and challenges current conservation strategies. Effective conservation requires vulnerability assessments for species susceptible to climate change and adaptive strategies to mitigate threats associated with climate. In this paper, we used the Maxent to model the impacts of climate change on habitat suitability of Sichuan golden monkey Rhinopithecus roxellana.

Correction: PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines.

[This corrects the article DOI: 10.1371/journal.pone.

Assessing vulnerability of giant pandas to climate change in the Qinling Mountains of China.

Climate change might pose an additional threat to the already vulnerable giant panda (). Effective conservation efforts require projections of vulnerability of the giant panda in facing climate change and proactive strategies to reduce emerging climate-related threats. We used the maximum entropy model to assess the vulnerability of giant panda to climate change in the Qinling Mountains of China.

Transcriptomic Response of Chinese Yew () to Cold Stress.

is a rare and endangered shrub, highly sensitive to temperature changes and widely known for its potential in cancer treatment. How gene expression of responds to low temperature is still unknown. To investigate cold response of the genus , we obtained the transcriptome profiles of grown under normal and low temperature (cold stress, 0°C) conditions using Illumina Miseq sequencing.

Range-Wide Snow Leopard Phylogeography Supports Three Subspecies.

The snow leopard, Panthera uncia, is an elusive high-altitude specialist that inhabits vast, inaccessible habitat across Asia. We conducted the first range-wide genetic assessment of snow leopards based on noninvasive scat surveys. Thirty-three microsatellites were genotyped and a total of 683 bp of mitochondrial DNA sequenced in 70 individuals.

PAMs ameliorates the imiquimod-induced psoriasis-like skin disease in mice by inhibition of translocation of NF-κB and production of inflammatory cytokines.

Psoriasis is a chronic and persistent inflammatory skin disease seriously affecting the quality of human life. In this study, we reported an ancient formula of Chinese folk medicine, the natural plant antimicrobial solution (PAMs) for its anti-inflammatory effects and proposed the primary mechanisms on inhibiting the inflammatory response in TNF-α/IFN-γ-induced HaCaT cells and imiquimod-induced psoriasis-like skin disease mouse model. Two main functional components of hydroxysafflor Yellow A and allantoin in PAMs were quantified by HPLC to be 94.

The microbially mediated soil organic carbon loss under degenerative succession in an alpine meadow.

Land-cover change has long been recognized as having marked effect on the amount of soil organic carbon (SOC). However, the microbially mediated processes and mechanisms on SOC are still unclear. In this study, the soil samples in a degenerative succession from alpine meadow to alpine steppe meadow in the Qinghai-Tibetan Plateau were analysed using high-throughput technologies, including Illumina sequencing and geochip functional gene arrays.

Variations of Soil Microbial Community Structures Beneath Broadleaved Forest Trees in Temperate and Subtropical Climate Zones.

Global warming has shifted climate zones poleward or upward. However, understanding the responses and mechanism of microbial community structure and functions relevant to natural climate zone succession is challenged by the high complexity of microbial communities. Here, we examined soil microbial community in three broadleaved forests located in the Wulu Mountain (WLM, temperate climate), Funiu Mountain (FNM, at the border of temperate and subtropical climate zones), or Shennongjia Mountain (SNJ, subtropical climate).

Soil bacterial endemism and potential functional redundancy in natural broadleaf forest along a latitudinal gradient.

Microorganisms play key roles in ecosystem processes and biogeochemical cycling, however, the relationship between soil microbial taxa diversity and their function in natural ecosystems is largely unknown. To determine how soil bacteria community and function are linked from the local to regional scale, we studied soil bacteria community composition, potential function and environmental conditions in natural and mature broadleaf forests along a latitudinal gradient in China, using the Illumina 16S rRNA sequencing and GeoChip technologies. The results showed strong biogeographic endemism pattern in soil bacteria were existed, and the spatial distance and climatic variables were the key controlling factors for this pattern.

Analyses of the influencing factors of soil microbial functional gene diversity in tropical rainforest based on GeoChip 5.0.

To examine soil microbial functional gene diversity and causative factors in tropical rainforests, we used a microarray-based metagenomic tool named GeoChip 5.0 to profile it. We found that high microbial functional gene diversity and different soil microbial metabolic potential for biogeochemical processes were considered to exist in tropical rainforest.

Soil organic matter quantity and quality shape microbial community compositions of subtropical broadleaved forests.

As two major forest types in the subtropics, broadleaved evergreen and broadleaved deciduous forests have long interested ecologists. However, little is known about their belowground ecosystems despite their ecological importance in driving biogeochemical cycling. Here, we used Illumina MiSeq sequencing targeting 16S rRNA gene and a microarray named GeoChip targeting functional genes to analyse microbial communities in broadleaved evergreen and deciduous forest soils of Shennongjia Mountain of Central China, a region known as 'The Oriental Botanic Garden' for its extraordinarily rich biodiversity.