Publications by authors named "Diogo de Oliveira Pessoa"
Article Synopsis
- HCMV genes play opposing roles in managing latency and reactivation in CD34 human progenitor cells (HPCs), as shown in an RNA sequencing study using the THP-1 cell line.
- Loss of certain genes increases viral gene expression and cell differentiation supporting HCMV, while their presence reduces viral gene expression during latency establishment.
- Transcriptional analysis indicates that host transcription factors may work with specific HCMV genes to regulate viral expression and potentially influence hematopoietic differentiation.
Salivary gland hypofunction is an adverse side effect associated with radiotherapy for head and neck cancer patients. This study delineated metabolic changes at acute, intermediate, and chronic radiation damage response stages in mouse salivary glands following a single 5 Gy dose. Ultra-high performance liquid chromatography-mass spectrometry was performed on parotid salivary gland tissue collected at 3, 14, and 30 days following radiation (IR).
View Article and Find Full Text PDFPurpose: Transcriptome analysis of pancreatic ductal adenocarcinoma (PDAC) has been useful to identify gene expression changes that sustain malignant phenotypes. Yet, most studies examined only tumor tissues and focused on protein-coding genes, leaving long non-coding RNAs (lncRNAs) largely underexplored.
Methods: We generated total RNA-Seq data from patient-matched tumor and nonmalignant pancreatic tissues and implemented a computational pipeline to survey known and novel lncRNAs.
The microenvironment of solid tumors is dynamic and frequently contains pockets of low oxygen levels (hypoxia) surrounded by oxygenated tissue. Indeed, a compromised vasculature is a hallmark of the tumor microenvironment, creating both spatial gradients and temporal variability in oxygen availability. Notably, hypoxia associates with increased metastasis and poor survival in patients.
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- Epigenetic changes, such as DNA methylation, play a crucial role in the development and progression of melanoma, alongside genetic mutations.* -
- The study aimed to discover how methylation of promoter and gene body regions affects gene expression, linking these changes to various stages of melanoma progression using a linear mouse model.* -
- Key genes related to tumor growth (Adcy3) and metastasis (Inpp4b) were identified, and the findings showed a correlation between the mouse model's results and clinical data from melanoma patient cohorts, suggesting possible prognostic markers.*
Article Synopsis
- Despite improvements in treatment, melanoma still often leads to poor patient outcomes, and there is a lack of effective biological models to study its progression.
- Researchers analyzed the gene expression profiles of various melanoma cell lines representing different stages of the disease, finding distinct gene expression patterns linked to cell differentiation and mesenchymal transitions.
- The study identified several genes that could serve as prognostic markers for melanoma progression, enhancing our understanding of the disease and suggesting potential new drug targets.
Radiation therapy for head and neck cancer causes damage to the surrounding salivary glands, resulting in salivary gland hypofunction and xerostomia. Current treatments do not provide lasting restoration of salivary gland function following radiation; therefore, a new mechanistic understanding of the radiation-induced damage response is necessary for identifying therapeutic targets. The purpose of the present study was to investigate the metabolic phenotype of radiation-induced damage in parotid salivary glands by integrating transcriptomic and metabolomic data.
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