The Value of BRAF VE1 Immunoexpression in Pediatric Langerhans Cell Histiocytosis.

VE1 is a monoclonal antibody detecting mutant BRAF V600E protein by immunohistochemistry (IHC) with a high concordance rate with molecular analysis in many cancers. BRAF V600E mutation was assessed on 94 pediatric LCH patients using sequencing analysis and VE1 immunohistochemistry with stringent and lenient-scoring criteria. BRAF V600E mutation exon 15 was detected by sequencing in 47.