Publications by authors named "Dingyuan Bai"
Hydroxytyrosol, a fine chemical, is widely utilized in food and pharmaceutical industries. In this study, we constructed a pathway to produce hydroxytyrosol by co-expressing tyrosin-phenol lyase (TPL), L-amino acid dehydrogenase (aadL), α-keto acid decarboxylase (KAD), aldehyde reductase (yahK) and glucose dehydrogenase (gdh). We changed combinations between plasmids with different copy numbers and target genes, resulting in 84% increase in hydroxytyrosol production.
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- Plasmid-based microbial systems are important for producing pharmaceuticals, but they usually need antibiotics for stability.
- Researchers created a new strain called A06Δ by relocating an essential gene and linking it with a specific enzyme on the same plasmid, aiming to remove the antibiotic requirement.
- The modified strain A17Δ outperformed A06Δ in producing phenylpyruvic acid (PPA), yielding 4.1 g L compared to 2.1 g L, and maintained plasmid stability for 10 transfers without antibiotics, eventually producing 18.7 g L of PPA in just 14 hours.
Exploring Multiple Goals Balancing in Complex Problem Solving Based on Log Data.
Front Psychol
September 2019
Multiple goals balancing is an important but not yet fully validated dimension of complex problem solving (CPS). The present study used process data to explore how solvers clarify goals, set priorities, and balance conflicting goals. We extracted behavioral indicators of goal pursuit from the log data of 3,201 students on the third subtask of the "Ticket" task in the PISA 2012 CPS test.
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