Synthesis of transfer ribonucleic acids with uridine or 2'-O-methylribothymidine at position 54 in developing Dictyostelium discoideum.

In amoebae of Dictyostelium discoideum the ribothymidine (rT) content of tRNA is 0.9 mol%, but decreases progressively during development into spores. To elucidate which nucleosides replace rT at position 54 in developmental tRNA we have characterized 'vegetative' and 'developmental' tRNAs from the slime mould.