Publications by authors named "Ding-Ran Wang"
Background: Endometrial cancer is one of the most commonly diagnosed gynecological cancers worldwide, and early-stage high-risk endometrial cancer has a poor prognosis. Adjuvant treatments after surgery, such as chemotherapy and radiotherapy, have been widely used in clinical practice to improve patient survival. Medroxyprogesterone acetate is a synthetic progestogen that has been reported to have potential anticancer effects in endometrial cancer.
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- * A new variant called BxHep-I was cloned and overexpressed, showing a high activity of 7144 U/L under optimized conditions, with a specific activity of 57.6 U/mg at 30°C and pH 7.5.
- * BxHep-I demonstrated excellent thermostability and retained 73% activity after one week at 4°C, making it a strong candidate for industrial applications in LMWH production.
Combination of engineering the substrate and Ca binding domains of heparinase I to improve the catalytic activity.
Prep Biochem Biotechnol
November 2023
Article Synopsis
- Heparinase I is an enzyme with promise for producing low molecular weight heparin, but its industrial use is limited due to poor catalytic activity and thermal stability.! -
- Researchers engineered specific domains of heparinase I and selected nine mutations to enhance its activity, with the T250D mutation showing the best results.! -
- The most successful mutant demonstrated a 5.26 times increase in catalytic efficiency, and the study suggests that this method could be useful for improving other enzymes as well.!