Objective: Based on the important medicinal applications of artemisinic acid and the superiority of Thin Layer Chromagraphy (TLC), the spot area method of TLC was presented to determine the content changes of artemisinic acid of Artemisia annua at different growing stages.
Methods: The separation conditions including chromatographic solutions and chromogenic agent were optimized. The detection limit and the linear concentration range were analyzed.
Objective: To study the variation of content of Artemisic acid of Artemisia annua from eight areas of four provinces around Wuling Mountain.
Methods: Artemisic acid of plants were extracted by organic solvent method. Qualitative and quantitative analysis of Artemisic acid were measured by thin layer chromatography (TLC) and high performance thin layer chromatography (HPLC), respectively.