Immobilization of horseradish peroxidase on UiO-66-NH for colorimetric and fluorometric sensing of nitrite.

Immobilized enzymes play a crucial role in analytical sensing due to their exceptional stability and considerable commercial importance. In this study, a stable Zr-based metal-organic framework (UiO-66-NH) was prepared as an immobilization platform for horseradish peroxidase (HRP) through covalent binding. HRP@UiO-66-NH retained 75% of its activity after 10 cycles.

Assessing validity and reliability of the Transition Shock Scale for Undergraduate Nursing Students (TSS, Chinese version) in associate degree nursing students.

Background: The Transition Shock Scale for Undergraduate Nursing Students assesses transition to undergraduate nursing students. The TSS is a tool used in various countries and has been translated into Chinese; however, associate degree nurses dominate China's nursing workforce, it needs to be validated in associate degree nursing interns. This study aimed to analyze the TSS (Chinese version) validity and reliability in Chinese associate degree nursing interns.

Interleukin-1 Receptor-Associated Kinase-3 Aggravates Neuroinflammatory Injury After Intracerebral Hemorrhage via Activation NF-κB/IL-17A Pathway in Mice.

Background: Neuroinflammatory reactions are crucial factors in secondary brain damage following intracerebral hemorrhage (ICH). Although previous studies have shown that IRAK3 is involved in immune responses, the potential effects of IRAK3 on ICH remain unclear.

Methods: Collagenase IV-induced ICH mouse model.

Formulation of Ethyl Cellulose Nanoparticles Encapsulated with Osthole Provides Long-Lasting Plant Protection against a Major Pest Mite.

The broader use of botanical pesticides has been limited by shorter residual activity on plants, slower onset of action, and higher costs compared with conventional pesticides. These challenges could be overcome by the development of simple, cost-effective, and long-lasting preventive nanocomposites for botanical pesticides. In this study, we successfully developed a low-cost ethyl cellulose (EC)-based delivery system for the botanical pesticide osthole (OST), designed to provide extended preventive protection against infestations.

Cell wall dynamic changes and signaling during plant lateral root development.

Lateral roots (LRs), are an important component of plant roots, playing a crucial role in anchoring the plant in the soil and facilitating the uptake of water and nutrients. As post-embryonic organs, LRs originate from the pericycle cells of the primary root, and their formation is characterized by precise regulation of cell division and complex intercellular interactions, both of which are closely tied to cell wall regulation. Considering the rapid advances in molecular techniques over the past three decades, we reframe the understanding of the dynamic change in cell wall during LR development by summarizing the factors that precipitate these changes and their effects, as well as the regulated signals involved.

Screening of key genes related to autophagy in psoriasis based on gene expression profiling.

Introduction: Autophagy is necessary for the progression of psoriasis.

Aim: This study aimed to recognize possible autophagy-related genes in psoriasis via bioinformatics study to present a better standard for the clinical treatment and management of psoriasis.

Material And Methods: The GEO dataset was utilized to derive the mRNA expression profile of the database GSE78097.

The role of circular RNAs in autoimmune diseases: Potential diagnostic biomarkers and therapeutic targets.

With the emergence of high-quality sequencing technologies, further research on transcriptomes has become possible. Circular RNA (circRNA), a novel type of endogenous RNA molecule with a covalently closed circular structure through "back-splicing," is reported to be widely present in eukaryotic cells and participates mainly in regulating gene and protein expression in various ways. It is becoming a research hotspot in the non-coding RNA field.

Application of DynaCT biliary soft tissue reconstruction technology in diagnosis and treatment of hepatolithiasis.

Background: Hepatobiliary stone disease involves an intrahepatic bile duct stone that occurs above the confluence of the right and left hepatic ducts. One-step percutaneous transhepatic cholangioscopic lithotripsy (PTCSL) using the percutaneous transhepatic one-step biliary fistulation (PTOBF) technique enables the clearing of intrahepatic stones and the resolution of strictures. However, hepatolithiasis with associated strictures still has high residual and recurrence rates after one-step PTCSL.

Laparoscopic hepatectomy using indocyanine green attenuates postoperative inflammatory response for hepatocellular carcinoma: A propensity score matching analysis.

Background: Improving the intraoperative and postoperative performance of laparoscopic hepatectomy was quite a challenge for liver surgeons.

Aim: To determine the benefits of indocyanine green (ICG) fluorescence imaging in patients with hepatocellular carcinoma (HCC) who underwent laparoscopic hepatectomy during and after surgery.

Methods: We retrospectively collected the clinicopathological data of 107 patients who successfully underwent laparoscopic hepatectomy at Zhongshan Hospital (Xiamen), Fudan University from June 2022 to June 2023.

Plasma membrane-associated ARAF condensates fuel RAS-related cancer drug resistance.

RAF protein kinases are major RAS effectors that function by phosphorylating MEK. Although all three RAF isoforms share a conserved RAS binding domain and bind to GTP-loaded RAS, only ARAF uniquely enhances RAS activity. Here we uncovered the molecular basis of ARAF in regulating RAS activation.

Exploring Surface State and Exciplex Dominated Aggregation Induced Electrochemiluminescence of Graphene Quantum Dots Prepared Via Electrochemical Exfoliation.

Graphene quantum dots (GQDs) have emerged as promising materials for electrochemiluminescence (ECL) applications due to their unique optical and electronic properties. In this study, GQDs were synthesized via electrochemical exfoliation of graphite in a constant current density mode, enabling scalable production with controlled size and surface functionalization. GQDs-4 and GQDs-20, synthesized at applied current densities of 4 mA/cm2 and 20 mA/cm2 to the graphite electrode, respectively, were investigated on roles of surface states and exciplex dominated aggregation-induced emission (AIE) in their ECL performance.

Characterization of a novel D-sorbitol dehydrogenase from Faunimonas pinastri A52C2.

The enzyme D-sorbitol dehydrogenase (SLDH) facilitates the conversion of D-sorbitol to L-sorbose. While current knowledge of this enzyme class predominantly centers on Gluconobacter oxydans, the catalytic properties of enzymes from alternative sources, particularly their substrate specificity and coenzyme dependency, remain ambiguous. In this investigation, we conducted BLASTp analysis and screened out a novel SLDH (Fpsldh) from Faunimonas pinastri A52C2.

Continuous atrial fibrillation monitoring using a wearable smartwatch: Using long-term Holter as reference.

Background: Wearables satisfactorily detect atrial fibrillation (AF) longer than 1 hour. Our study aims to evaluate smartwatch performances for long-term AF monitoring, including AF with short durations.

Methods: This prospective study enrolled AF patients from 2020 to 2023.

Finite Element Analysis of Proximal Femoral Bionic Nail (PFBN), Proximal Femoral Nail Antirotation and InterTan for Treatment of Reverse Obliquity Intertrochanteric Fractures.

Objective: Reverse obliquity intertrochanteric fracture is an unstable type of fracture. Current guidelines recommend intramedullary fixation, but there are still complications such as screw removal, hip varus, nail withdrawal, and nail fracture. The objective of this study was to use finite element analysis to compare the biomechanical properties of the novel proximal femoral bionic nail (PFBN), proximal femoral nail antirotation (PFNA), and combined compression interlocking intramedullary nail (InterTan) in the treatment of reverse obliquity intertrochanteric fractures (AO/OTA 31-A3.

Mid- to long-term clinical outcomes of modified technique skip-level titanium plate fixation in cervical laminoplasty compared to continuous fixation.

Purpose: To compare the efficacy and safety of skip titanium plates combined with adjacent spinous process suture suspension versus continuous titanium plate fixation in cervical laminoplasty.

Methods: A retrospective analysis of 125 patients (62 men, 63 women, average age 60.9 ± 10.

HP1 Promotes the Centromeric Localization of ATRX and Protects Cohesion by Interfering Wapl Activity in Mitosis.

Background: α thalassemia/mental retardation syndrome X-linked (ATRX) serves as a part of the sucrose nonfermenting 2 (SNF2) chromatin-remodeling complex. In interphase, ATRX localizes to pericentromeric heterochromatin, contributing to DNA double-strand break repair, DNA replication, and telomere maintenance. During mitosis, most ATRX proteins are removed from chromosomal arms, leaving a pool near the centromere region in mammalian cells, which is critical for accurate chromosome congression and sister chromatid cohesion protection.

Silybin Cocrystals with Improved Solubility and Bioavailability.

Silymarin, an extract from milk thistle, is widely recognized for its therapeutic potential in treating liver disorders. However, its clinical utility is limited by the poor solubility and low bioavailability of its key active ingredient, Silybin. In this study, we sought to address this issue through the development of a novel cocrystal of Silyin.

Lipid Profiles, Telomere Length, and the Risk of Malignant Tumors: A Mendelian Randomization and Mediation Analysis.

The relationship between lipid profiles, telomere length (TL), and cancer risk remains unclear. This study employed two-sample Mendelian randomization (MR) with mediation analysis to investigate their causal relationships, examining lipid profiles as exposure, TL as mediator, and nine cancer types as outcomes. We conducted our analysis using two-stage least squares (2SLS) regression integrated with inverse variance weighted (IVW) methods to address potential endogeneity and strengthen our causal inference.

The effect of healthy eating index-2015 in the associations of biological aging and non-alcoholic fatty liver disease: an interaction and mediation analysis.

Background: The present study explored the association between biological aging (BA), healthy eating index-2015 (HEI-2015) and non-alcoholic fatty liver disease (NAFLD) in the general population of the United States.

Methods: We used data from the NHANES database between 2017-2018 years to conduct the study. Weighted multivariable logistic regression analysis, restricted cubic spline (RCS), and subgroup analysis were performed to analyze the association of BA and HEI-2015 with prevalence of NAFLD and the mediation effect of HEI-2015 was also discussed.

Disrupting of IGF2BP3-stabilized CLDN11 mRNA by TNF-α increases intestinal permeability in obesity-related severe acute pancreatitis.

Background: Obesity is a significant risk factor for severe acute pancreatitis (SAP) and is typically associated with increased intestinal permeability. Understanding the role of specific molecules can help reduce the risk of developing SAP. Claudin 11 (CLDN11), a member of the Claudin family, regulates the permeability of various internal barriers.

Maize kernel nutritional quality-an old challenge for modern breeders.

This article offers a comprehensive overview of the starch, protein, oil, and carotenoids content in maize kernels, while also outlining future directions for research in this area. Maize is one of the most important cereal crops globally. Maize kernels serve as a vital source of feed and food, and their nutritional quality directly impacts the dietary intake of both animals and humans.

Alterations in Muscle Coordination to Reduce Knee Joint Loading for People with Limb Loss.

Purpose: People with unilateral transtibial amputation experienced a higher incidence of knee osteoarthritis due to abnormal movement patterns. This study aimed to explore alternations in muscle coordination to reduce mechanical burdens in their daily activities.

Methods: Five males with unilateral transtibial amputation were recruited.

Deep-Learning Generated Synthetic Material Decomposition Images Based on Single-Energy CT to Differentiate Intracranial Hemorrhage and Contrast Staining Within 24 Hours After Endovascular Thrombectomy.

Aims: To develop a transformer-based generative adversarial network (trans-GAN) that can generate synthetic material decomposition images from single-energy CT (SECT) for real-time detection of intracranial hemorrhage (ICH) after endovascular thrombectomy.

Materials: We retrospectively collected data from two hospitals, consisting of 237 dual-energy CT (DECT) scans, including matched iodine overlay maps, virtual noncontrast, and simulated SECT images. These scans were randomly divided into a training set (n = 190) and an internal validation set (n = 47) in a 4:1 ratio based on the proportion of ICH.