Dual cytoplasmic-peroxisomal compartmentalization engineering and multiple metabolic engineering strategies for high yield non-psychoactive cannabinoid in Saccharomyces cerevisiae.

CBG (Cannabigerol), a nonpsychoactive cannabinoid, has garnered attention due to its extensive antimicrobial and anti-inflammatory properties. However, the natural content of CBG in Cannabis sativa L. is minimal.

Semisynthesis of Plant-Derived Englerin A Enabled by Microbe Engineering of Guaia-6,10(14)-diene as Building Block.

Herein, we report a short semisynthesis of the potent transient receptor potential canonical (TRPC) channel agonist englerin A (EA) and the related guaianes oxyphyllol and orientalol E. The guaia-6,10(14)-diene starting material was systematically engineered in and using the CRISPR/Cas9 system and was produced with high titers. The potentially scalable approach combines the advantages of synthetic biology and chemical synthesis providing an efficient and economical method for producing EA and analogues.

Overproduction of gentamicin B in industrial strain CCTCC M 2018898 by cloning of the missing genes and .

In pharmaceutical industry, isepamicin is mainly manufactured from gentamicin B, which is produced by as a minor component of the gentamicin complex. Improvement of gentamicin B production through metabolic engineering is therefore important to satisfy the increasing demand for isepamicin. We hypothesized that gentamicin B was generated from gentamicin JI-20A via deamination of the C2' amino group.

Systematic Metabolic Engineering of for Lycopene Overproduction.

Lycopene is widely used in foods, cosmetics, nutritional supplements, and pharmaceuticals. Microbial production of lycopene has been intensively studied. However, there are few systematic engineering studies on aimed at achieving high-yield lycopene production.

A wide dynamic range x-ray streak camera system.

An x-ray streak camera with wide dynamic range and a large slit photocathode of 30 mm length has been developed and calibrated. In order to achieve wide dynamic range, a conventional streak tube has been improved and the camera system has been designed without microchannel plate electron amplifier. As a result, a dynamic range of 922 is achieved in a single shot mode with laser pulse of 30 ps (full width at half maximum) at time resolution of better than 31 ps.