sp. nov. and sp. nov., two bacteria isolated from marine sediment in the East China Sea.

Two Gram-stain-negative, curved-rod-shaped, non-motile and aerobic bacteria W6 and I13 were isolated from marine sediment samples collected from Meishan Island located in the East China Sea. Catalase and oxidase activities and hydrolysis of Tween 40, 60 and 80 were positive for both strains, while nitrate reduction, indole production, methyl red reaction and HS production were negative. Phylogenetic analyses based on 16S rRNA and genome sequences revealed that strains W6 and I13 formed distinct phylogenetic lineages within the genera and , respectively.

Marine Staurosporine Analogues: Activity and Target Identification in Triple-Negative Breast Cancer.

Triple-negative breast cancer (TNBC) is a subtype of breast cancer with high mortality and drug resistance and no targeted drug available at present. Compound , a staurosporine alkaloid derived from sp. NBU3142 in a marine sponge, exhibits potent anti-TNBC activity.

The potential of marine natural products and their synthetic derivatives as drugs targeting ion channels.

Ion channels are a type of protein channel that play a vital role in numerous physiological functions by facilitating the passage of ions through cell membranes, thereby enabling ion and electrical signal transmission. As a crucial target for drug action, ion channels have been implicated in various diseases. Many natural products from marine organisms, such as fungi, algae, sponges, and sea cucumber, etc.

Antibiofilm Efficacy and Mechanism of the Marine Chlorinated Indole Sesquiterpene Against Methicillin-Resistant .

Methicillin-resistant (MRSA) can easily form biofilms on food surfaces, thus leading to cross-contamination, which is difficult to remove. Therefore, there is an urgent need to find alternatives with good antibacterial and antibiofilm effects. In this study, two indole sesquiterpene compounds, xiamycin () and chlorinated metabolite chloroxiamycin (), were isolated from the fermentation liquid of marine sp.

sp. nov., a novel member isolated from coastal mudflat sediment of Meishan Islandin the East China Sea.

A Gram-negative, rod-shaped, non-motile and strictly aerobic strain, designated NBU2979, was isolated from a coastal mudflat located on Meishan Island in the East China Sea. Strain NBU2979 grew optimally at 32 °C, with 2.0 % NaCl (w/v) and at pH 7.

Genomic Analysis of to Explore Its Biosynthetic Potential Regarding Secondary Metabolites.

Actinomycetes have long been recognized as important sources of clinical antibiotics. However, the exploration of rare actinomycetes, despite their potential for producing bioactive molecules, has remained relatively limited compared to the extensively studied genus. The extensive investigation of species and their natural products has led to a diminished probability of discovering novel bioactive compounds from this group.

Discovery of Antibacterial Compounds against subsp. from a Marine Fungus SCSIO 41202 and the Mode of Action.

Citrus canker, caused by subsp. (), is a severe citrus disease. Currently, copper-containing pesticides are widely used to manage this disease, posing high risks to the environment and human health.

Design of a Novel Lysine Isopeptide 1018KI11 with Potent Antimicrobial Activity as a Safe and Stable Food Preservative Candidate.

Antimicrobial peptides are potent food additive candidates, but most of them are sensitive to proteases, which limits their application. Therefore, we substituted arginine for lysine and introduced a lysine isopeptide bond to peptide IDR-1018 in order to improve its enzymatic stability. Subsequently, the protease stability and antimicrobial/antibiofilm activity of the novel peptides (1018K2-1018KI11) were investigated.

Irpetones A and B, Anti-Osteoclastic Heptaketides from a Marine Mesophotic Zone Sponge-Associated Fungus sp. NBUF088.

Chemical investigation of sp. NBUF088, associated with an sp. sponge located at an 84 m deep mesophotic zone, led to the discovery of two new heptaketides, named irpetones A () and B ().

Research on adaptive state prediction method for the metering error of capacitor voltage transformer.

A capacitor voltage transformer (CVT) is widely used in high voltage power systems because of its good insulation performance. However, the structure of CVT is more complex and the stability of its metering error is poor, which easily causes the loss of power metering. The conventional evaluation method for the metering error of CVT is to compare and calibrate with a standard transformer under off-line condition in a fixed period.

sp. nov., a facultative anaerobic bacterium belonging to the family isolated from marine sediment in the East China Sea.

A facultative anaerobic, Gram-strain-negative, rod-shaped bacterium (strain NBU2970) was isolated by using modified ichip cultivation from a marine sediment sample collected from Meishan Island in the East China Sea. Strain NBU2970 grew optimally at 37 °C, with a NaCl concentration of 2.0 % (w/v) and at pH 7.

Antibacterial oxygenated ergostane-type steroids produced by the marine sponge-derived fungus sp.

Two oxygenated ergostane-type steroids including one new compound, 3-hydroxy-5,6-methoxyergosta-7,22-dien-15-one () along with a known analogue ergosta-6,22-dien-3,5,8-triol () were isolated from the crude extracts of the marine sponge-derived fungus sp. Their structures were elucidated on the basis of combined NMR and MS spectroscopic methods. Compound was a marine ergostane-type steroid with two methoxy groups at C-5 and C-6, respectively.

sp. nov. and sp. nov., two novel bacteria isolated from the pit mud of Chinese liquor.

Two pink-pigmented bacterial strains, designated NBU2971 and NBU2972, were isolated from the pit mud of a Chinese liquor. Phylogenetic analyses based on 16S rRNA gene sequences suggested that strains NBU2971 and NBU2972 formed a distinct lineage within the family and were closely related to members of the genus . 16S rRNA gene sequences revealed that strain NBU2971 showed highest similarity of 97.

Molecular networking-guided isolation of undescribed antifungal odoriferous sesquiterpenoids from a marine mesophotic zone sponge-associated Streptomyces sp. NBU3428.

Under the guidance of MS/MS-based molecular networking, eight odoriferous sesquiterpenes including two undescribed geosmin-type sesquiterpenoid degradations, odoripenoid A (1) and odoripenoid B (2), and two undescribed germacrane-type sesquiterpenoids, odoripenoid C (3) and odoripenoid (4), together with four known related compounds (5-8) were isolated from the EtOAc extract of the marine mesophotic zone sponge-associated Streptomyces sp. NBU3428. All chemical structures including absolute configurations of these compounds were elucidated by means of HRESIMS, NMR, ECD calculations and single-crystal X-ray diffraction experiments.

Discovery of novel polysubstituted -alkyl acridone analogues as histone deacetylase isoform-selective inhibitors for cancer therapy.

Pan-histone deacetylase (HDAC) inhibitors often have some toxic side effects. In this study, three series of novel polysubstituted -alkyl acridone analogous were designed and synthesised as HDAC isoform-selective inhibitors. Among them, and exhibited selective inhibition of HDAC1, HDAC3, and HDAC10, with IC values ranging from 87 nM to 418 nM.

sp. nov. and sp. nov., two novel species in the family isolated by a modified cultivation technique from marine sediment.

A modified cultivation technique was developed and applied to resource mining of uncultured microbes from marine sediments of Meishan Island in the East China Sea. Two novel strains NBU2968 and NBU2984 were isolated by this method but not standard Petri dish, which indicated the modified technique was more effective compared to conventional approaches for isolating uncultured microbes and could be popularized and applied to other aquatic environments. The two novel strains were identified by the polyphasic taxonomic approach.

Novel Antimycin Analogues with Agricultural Antifungal Activities from the Sponge-Associated Actinomycete sp. NBU3104.

Phytopathogenic fungi could affect the growth of agricultural products and result in serious economic losses. To develop novel and potent fungicides, secondary metabolites of an oceanic mesophotic zone sp. NBU3104 was isolated by metabolomics and genomics, which led to the discovery of eight novel antimycins I-P (-), including antimycin I (), six rare acetylated actimycins J-N (-), P (), and an unusual deformylated antimycin O ().

Guignardones Y-Z, Antiviral Meroterpenes from Penicillium sp. NBUF154 Associated with a Crella Sponge from the Marine Mesophotic Zone.

Guignardones Y-Z (1-2), two new meroterpenoids, and six known metabolites involving guignardones A-H (3-4), gyorgy-isoflavone (5), daidzein (6), blumenol A (7) and guignardianone A (8) were isolated from the fungus Penicillium sp. NBUF154, which was obtained from a 60 m deep Crella sponge. Their structures including absolute configurations were unambiguously elucidated by exhaustive spectroscopic analysis and ECD calculations.

Marine natural products and their synthetic analogs as promising antibiofilm agents for antibiotics discovery and development.

Biofilm is a complex microbial consortium that are embedded in a membrane structure of the extracellular polymeric substances (EPS). As a major form of microorganisms in nature, biofilm has evolved complex and diverse resistance mechanisms to numerous known antibiotics, posing a major threat to human health. The biofilm formation of pathogens including Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans, etc.

sp. nov., a novel bacterium isolated from marine sediment, and emended description of the genus .

A Gram-stain-negative, rod-shaped, motile and aerobic marine bacterium, designated strain NBU2595, was isolated from marine sediment sampled on Meishan Island, located in the East China Sea. Strain NBU2595 grew at 10-40 °C (optimum, 37 °C), at NaCl concentration of 0-10.0 % (w/v; optimum, 0.

Molecular Networking-Driven Discovery of Antibacterial Perinadines, New Tetracyclic Alkaloids from the Marine Sponge-Derived Fungus sp.

Two rare tetracyclic skeleton alkaloids named perinadines B and C ( and ) were isolated as mixtures of epimers from the marine-derived sp. LS116 driven by molecular networking. The planar structures of and were characterized by comprehensive spectroscopic data.

Novel anti-hyperuricemic hexapeptides derived from hydrolysate and their modulation effects on the gut microbiota and host microRNA profile.

Hyperuricemia (HUA) is the second most common metabolic disease nowadays, and is characterized by permanently increased concentrations of serum uric acid. In this study, two novel hexapeptides (GPAGPR and GPSGRP) were identified from hydrolysate and predicted to have xanthine oxidase (XOD) inhibitory activity by molecular docking. Their XOD inhibition rates reached 37.

Feature-based molecular networking-guided discovery of siderophores from a marine mesophotic zone Axinellida sponge-associated actinomycete Streptomyces diastaticus NBU2966.

Ten siderophore-related compounds were isolated by MS/MS-based molecular networking from a mesophotic sponge-associated Streptomyces diastaticus NBU2966. Among them, there were three kinds of siderophores including three undescribed phenol/thiazoline-type compounds, thiazostatin C, methyl thiazostatin B, and pulicatin J, one undescribed phenol/oxazoline-type compound, spoxazomicin E, and one undescribed phenol/oxazoline/thiazoline-type compound, streptochelin A, as well as five known compounds. Pulicatin J and spoxazomicin E were both isolated as a pair of inseparable epimers.