Poor Agreement Between Next-Generation DNA Sequencing and Bacterial Cultures in Orthopaedic Trauma Procedures.

Background: Next-generation DNA sequencing (NGS) detects bacteria-specific DNA corresponding to the 16S ribosomal RNA gene and can identify bacterial presence with greater accuracy than traditional culture methods. The clinical relevance of these findings is unknown. The purpose of the present study was to compare the results from bacterial culture and NGS in order to characterize the potential use of NGS in orthopaedic trauma patients.

Typhoon-boosted biogenic emission aggravates cross-regional ozone pollution in China.

Ozone pollution that threatens human health and the ecosystem is a global environmental challenge. In megacities, ozone pollution has long been mainly attributed to anthropogenic sources. However, the processes and mechanisms of cross-regional transport of ozone and its precursors under interactions between mixed sources remain unclear.

Ferroptosis-related gene NOX4, CHAC1 and HIF1A are valid biomarkers for stomach adenocarcinoma.

Ferroptosis is a regulated cell death nexus linking metabolism, redox biology and diseases including cancer. The aim of the present study was to identify a ferroptosis-related gene prognostic signature for stomach adenocarcinoma (STAD) by systematic analysis of transcriptional profiles from The Cancer Genome Atlas (TCGA), GEO and a clinical cohort from our centre. We developed a predictive model based on three ferroptosis-related genes (CHAC1, NOX4 and HIF1A), gene expression data and corresponding clinical outcomes were obtained from the TCGA database, and the reliability of this model was verified with GSE15459 and 51 queues in our centre.

Cement kiln co-processing promotes the redevelopment of industrially contaminated land in China: Spatio-temporal features and efficiency analysis.

The cleanup and redevelopment of industrially contaminated land are essential for sustainable urban development, both from economic and environmental perspectives. Remediation efforts in developed countries have incurred high decontamination costs and administrative, monitoring, and enforcement requirements that are beyond the capabilities of many developing nations. Here, we reviewed the development of cement kiln co-processing of contaminated soils, a commonly used remediation strategy in China, and discussed the spatio-temporal features, benefits and efficiency of co-processing.

In-situ utilization of membrane foulants (FeO+MnO) for the efficient membrane cleaning.

Preoxidation-ultrafiltration process is an effective method for Fe and Mnremoval, in which Fe (Mn) are firstly oxidized to FeO (MnO), then collected by the ultrafiltration membrane. However, the simultaneous presence of Fe, Mn, and organics in feed can cause severe membrane fouling, which inhibits the overall performance of this method prominently. In this study, a novel FeO+MnO+HO membrane cleaning method is proposed based on the idea of turning in-situ generated membrane foulants, i.

The effect of urban morphological characteristics on the spatial variation of PM air quality in downtown Nanjing.

The effects of the urban morphological characteristics on the spatial variation of near-surface PM air quality were examined. Unlike previous studies, we performed the analyses in real urban environments using continuous observations covering the whole scale of urban densities typically found in cities. We included data from 31 measurement stations divided into 8 different wind sectors with individually defined morphological characteristics leading to highly varying urban characteristics.

Ecological Insights Into Community Interactions, Assembly Processes and Function in the Denitrifying Phosphorus Removal Activated Sludge Driven by Phosphorus Sources.

The microbial characteristics in the wastewater treatment plants (WWTPs) strongly affect their optimal performance and functional stability. However, a cognitive gap remains regarding the characteristics of the microbial community driven by phosphorus sources, especially co-occurrence patterns and community assembly based on phylogenetic group. In this study, 59 denitrifying phosphorus removal (DPR) activated sludge samples were cultivated with phosphorus sources.

Development and Assessment of a High-Resolution Biogenic Emission Inventory from Urban Green Spaces in China.

Biogenic volatile organic compound (BVOC) emissions have long been known to play vital roles in modulating the formation of ozone and secondary organic aerosols (SOAs). While early studies have evaluated their impact globally or regionally, the BVOC emissions emitted from urban green spaces (denoted as U-BVOC emissions) have been largely ignored primarily due to the failure of low-resolution land cover in resolving such processes, but also because their important contribution to urban BVOCs was previously unrecognized. In this study, by utilizing a recently released high-resolution land cover dataset, we develop the first set of emission inventories of U-BVOCs in China at spatial resolutions as high as 1 km.

Phantom assessment of three-dimensional geometric distortion of a dedicated wide-bore MR-simulator for radiotherapy.

This study evaluated the machine-dependent three-dimensional geometric distortion images acquired from a 1.5T 700 mm-wide bore MR-simulator based on a large geometric accuracy phantom. With the consideration of radiation therapy (RT) application requirements, every sequence was examined in various combinations of acquisition-orientations and receiver-bandwidths with console-integrated distortion correction enabled.

Key Performance Indicators for Quality Imaging Practice: Why, What, and How.

A common trend across health care organizations is the development of key performance indicators (KPIs) for characterizing quality, identifying areas in need of change, and quantifying the impact of change. This article outlines a list of KPIs that can be used to quantify, target, and optimize value and value delivery in medical imaging practice. Of particular focus here is the aspect of practice that should be overseen and informed by the work of medical physicists, along the trajectory and expectations of a Medical Physics 3.

Quantitative proteomics insights into gel properties changes of myofibrillar protein from Procambarus clarkii under cold stress.

The impacts of cold stress (4 ℃ for 0 h, 12 h, 24 h, 36 h and 48 h, respectively) on the components, structural and physical properties of myofibrillar protein (MP) gel from Procambarus clarkii were investigated. The physicochemical analysis indicated the secondary and tertiary structure of MP were unfolding to different degrees after cold stress when compared to the control. The MP gel hardness reached a maximum when the cold stress reached 24 h.

Unveiling the dipole synergic effect of biogenic and anthropogenic emissions on ozone concentrations.

Biogenic emissions are widely known as important precursors of ozone, yet there is potentially a strong interaction and synergy between biogenic and anthropogenic emissions, including volatile organic compounds (VOCs) and nitrogen oxides (NOx), in modulating ozone formation. To a large extent, the synergy affects the effectiveness of anthropogenic emission control, thereby reshaping the O-NOx-VOC empirical kinetic modeling approach (EKMA) diagram. Focusing on the ozone pollution period of June 2017 in the North China Plain, we design almost 500 numerical experiments using regional air quality model Community Multiscale Air Quality (CMAQ) that revealed an interesting synergic effect, defined as the contribution of biogenic emissions to ozone concentrations concomitant with a reduction in anthropogenic emissions.

Sensorineural Hearing Loss After Teprotumumab Therapy for Thyroid Eye Disease: A Case Report.

Background And Objective: Teprotumumab is a fully human IgG1 monoclonal antibody against insulin-like growth factor-1 receptor (IGF-1R). It has been approved by the FDA to treat thyroid eye disease (TED) by reducing extraocular muscle and orbital fat volume. In clinical trials, hearing loss was noted as a side effect in 7 to 12% of patients, but no formal audiometric investigations of these patients were reported.

Improvement of sludge dewaterability by energy uncoupling combined with chemical re-flocculation: Reconstruction of floc, distribution of extracellular polymeric substances, and structure change of proteins.

This study innovatively combines energy uncoupling and chemical re-flocculation helped to accelerate residual sludge dewatering. Ferric chloride (FeCl) and 3, 3', 4', 5-tetrachlor-osalicylanilide (TCS) were employed as the flocculant and uncoupler, respectively. The results showed that the specific resistance to filtration (SRF) and the water content of sludge filtered cake fell dramatically from 11 × 10 m/kg and 80.

Imaging identification of primary mammary analogue secretory carcinoma and acinic cell carcinoma in major salivary glands.

Objective: The present study aimed to characterize and differentiate the ultrasonography (US) and computed tomography (CT) features of mammary analogue secretory carcinoma (MASC) and acinic cell carcinoma (AciCC).

Methods And Patients: A total of 83 patients with clinically proven MASC and AciCC were analyzed. The following characteristics were assessed on US, CT, and magnetic resonance imaging: lesion size, shape, margin, echogenicity, echotexture, cystic components, posterior echo, vascularity, density, degree of enhancement, enhancement pattern, signal intensity (SI) on T1- and T2-weighted images (WI), hemorrhages, and lymph node enlargement.

Generation of functional ciliated cholangiocytes from human pluripotent stem cells.

The derivation of mature functional cholangiocytes from human pluripotent stem cells (hPSCs) provides a model for studying the pathogenesis of cholangiopathies and for developing therapies to treat them. Current differentiation protocols are not efficient and give rise to cholangiocytes that are not fully mature, limiting their therapeutic applications. Here, we generate functional hPSC-derived cholangiocytes that display many characteristics of mature bile duct cells including high levels of cystic fibrosis transmembrane conductance regulator (CFTR) and the presence of primary cilia capable of sensing flow.

Pregnancy complications effect on the nickel content in maternal blood, placenta blood and umbilical cord blood during pregnancy.

Background: Nickel (Ni) may accumulate in the human body and has biological toxicity and carcinogenicity. Ni has an extensive impact on the health of pregnant women and fetuses during gestation.

Aim: To evaluate Ni exposure in pregnant women in Kunming, Yunnan Province, China; to describe the distribution of Ni in the maternal-fetal system and placental barrier function; and to investigate the effect of Ni exposure on fetal health in mothers with pregnancy complications.

Aerosol-boundary-layer-monsoon interactions amplify semi-direct effect of biomass smoke on low cloud formation in Southeast Asia.

Low clouds play a key role in the Earth-atmosphere energy balance and influence agricultural production and solar-power generation. Smoke aloft has been found to enhance marine stratocumulus through aerosol-cloud interactions, but its role in regions with strong human activities and complex monsoon circulation remains unclear. Here we show that biomass burning aerosols aloft strongly increase the low cloud coverage over both land and ocean in subtropical southeastern Asia.

Patient Satisfaction With the Head and Neck Cancer Telephone Triage Service During the COVID-19 Pandemic.

Background A telephone triage consultation, as part of the two-week wait head and neck cancer referral pathway, was implemented nationally in March 2020. This was in response to the COVID-19 pandemic to stream cancer referrals to minimize unnecessary interactions and appointments with health services. The aim of this study is to assess patient satisfaction with this novel telephone triage system in the setting of a district general hospital.

Transcriptional and epigenetic regulation of temporal patterning in neural progenitors.

During development, neural progenitors undergo temporal patterning as they age to sequentially generate differently fated progeny. Temporal patterning of neural progenitors is relatively well-studied in Drosophila. Temporal cascades of transcription factors or opposing temporal gradients of RNA-binding proteins are expressed in neural progenitors as they age to control the fates of the progeny.

Genome-Wide Identification and Low-Temperature Expression Analysis of bHLH Genes in .

is an illustrious ornamental woody plant with colorful flowers, delicate fragrances, and graceful tree forms. Low temperature limits its geographical distribution. The basic helix-loop-helix (bHLH) proteins exist in most eukaryotes as a transcription factor superfamily, which play a crucial role in metabolism, physiology, development, and response to various stresses of higher organisms.

The Implicit Aesthetic Preference for Mobile Marketing Interface Layout-An ERP Study.

Businesses and scholars have been trying to improve marketing effect by optimizing mobile marketing interfaces aesthetically as users browse freely and aimlessly through mobile marketing interfaces. Although the layout is an important design factor that affects interface aesthetics, whether it can trigger customer's aesthetic preferences in mobile marketing remains unexplored. To address this issue, we employ an empirical methodology of event-related potentials (EPR) in this study from the perspective of cognitive neuroscience and psychology.

MUD1, a RING-v E3 ubiquitin ligase, has an important role in the regulation of pectin methylesterification in Arabidopsis seed coat mucilage.

Pectin is one of the major components of plant primary cell wall polysaccharides. The degree of pectin methylesterification (DM) plays an important role in the process of plant growth. However, little is known about the underlying regulatory mechanisms during the process of pectin demethylesterification.

Effects of the metabolic uncoupler TCS on residual sludge treatment: Analyses of the microbial community and sludge dewaterability potential.

Residual sludge is a by-product with a large volume and complex composition from wastewater treatment plants. It is significant to reduce sludge volume to decrease the negative effects of sludge on environmental pollution and needless land use. We investigated the effects of uncoupler 3, 3', 4', 5-tetrachlorosalicylanilide (TCS) on the properties of sludge.

Ranging system based on optical carrier-based microwave interferometry.

To compensate for the refractive index errors in optical carrier-based microwave interferometry (OCMI), a ranging system is designed to measure the single-arm optical path of OCMI. A high-speed photodetector and a downconversion method are used to acquire the microwave envelope of the interference signal. A Hilbert transformation is used to realize phase detection.